Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O07165 (ENDA_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:MTH_250
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01833

Subunit structure

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 170170tRNA-splicing endonuclease HAMAP-Rule MF_01833
PRO_0000109473

Sites

Active site1061 By similarity
Active site1161 By similarity
Active site1471 By similarity

Sequences

Sequence LengthMass (Da)Tools
O07165 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 41FA9B6959447E0A

FASTA17019,693
        10         20         30         40         50         60 
MRVEGQLGDE VVTIKATSIA RRLHGKSHYG KMYEDRLQLS LIEAAYLMER GKLKLMKDDD 

        70         80         90        100        110        120 
EVSPEEFISL LGERGLYSKY LVYRDLRNRG YIVKTGFKYG AEFRLYERGG APGRTHSAYL 

       130        140        150        160        170 
VRVISENDTI HALDFSSYVR VAHGVNKKLL MAFLDDEEDI TYYLVDWIRP 

« Hide

References

« Hide 'large scale' references
[1]"Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems."
Kleman-Leyer K., Armbruster D.W., Daniels C.J.
Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001577 Genomic DNA. Translation: AAC45445.1.
AE000666 Genomic DNA. Translation: AAB84756.1.
PIRD69131.
RefSeqNP_275393.1. NC_000916.1.

3D structure databases

ProteinModelPortalO07165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO07165. 1 interaction.
STRING187420.MTH250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB84756; AAB84756; MTH_250.
GeneID1470211.
KEGGmth:MTH250.

Phylogenomic databases

eggNOGCOG1676.
KOK01170.
OMAALKFGSH.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-250-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01833. EndA_short.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENDA_METTH
AccessionPrimary (citable) accession number: O07165
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families