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O07165

- ENDA_METTH

UniProt

O07165 - ENDA_METTH

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei106 – 1061UniRule annotation
    Active sitei116 – 1161UniRule annotation
    Active sitei147 – 1471UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:MTH_250
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 170170tRNA-splicing endonucleasePRO_0000109473Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    IntActiO07165. 1 interaction.
    STRINGi187420.MTH250.

    Structurei

    3D structure databases

    ProteinModelPortaliO07165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    KOiK01170.
    OMAiALKFGSH.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01833. EndA_short.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O07165-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVEGQLGDE VVTIKATSIA RRLHGKSHYG KMYEDRLQLS LIEAAYLMER    50
    GKLKLMKDDD EVSPEEFISL LGERGLYSKY LVYRDLRNRG YIVKTGFKYG 100
    AEFRLYERGG APGRTHSAYL VRVISENDTI HALDFSSYVR VAHGVNKKLL 150
    MAFLDDEEDI TYYLVDWIRP 170
    Length:170
    Mass (Da):19,693
    Last modified:July 1, 1997 - v1
    Checksum:i41FA9B6959447E0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001577 Genomic DNA. Translation: AAC45445.1.
    AE000666 Genomic DNA. Translation: AAB84756.1.
    PIRiD69131.
    RefSeqiNP_275393.1. NC_000916.1.
    WP_010875889.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB84756; AAB84756; MTH_250.
    GeneIDi1470211.
    KEGGimth:MTH250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001577 Genomic DNA. Translation: AAC45445.1 .
    AE000666 Genomic DNA. Translation: AAB84756.1 .
    PIRi D69131.
    RefSeqi NP_275393.1. NC_000916.1.
    WP_010875889.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O07165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O07165. 1 interaction.
    STRINGi 187420.MTH250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB84756 ; AAB84756 ; MTH_250 .
    GeneIDi 1470211.
    KEGGi mth:MTH250.

    Phylogenomic databases

    eggNOGi COG1676.
    KOi K01170.
    OMAi ALKFGSH.

    Enzyme and pathway databases

    BioCyci MTHE187420:GJNM-250-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01833. EndA_short.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems."
      Kleman-Leyer K., Armbruster D.W., Daniels C.J.
      Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiENDA_METTH
    AccessioniPrimary (citable) accession number: O07165
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3