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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106UniRule annotation1
Active sitei116UniRule annotation1
Active sitei147UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:MTH_250
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094731 – 170tRNA-splicing endonucleaseAdd BLAST170

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

Protein-protein interaction databases

IntActiO07165, 1 interactor
STRINGi187420.MTH250

Structurei

3D structure databases

ProteinModelPortaliO07165
SMRiO07165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
KOiK01170
OMAiKYGSDFR
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01833 EndA_short, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR016442 tRNA_splic_arch_short
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF005285 tRNA_splic_archaea, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
SSF55267 SSF55267, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

O07165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVEGQLGDE VVTIKATSIA RRLHGKSHYG KMYEDRLQLS LIEAAYLMER
60 70 80 90 100
GKLKLMKDDD EVSPEEFISL LGERGLYSKY LVYRDLRNRG YIVKTGFKYG
110 120 130 140 150
AEFRLYERGG APGRTHSAYL VRVISENDTI HALDFSSYVR VAHGVNKKLL
160 170
MAFLDDEEDI TYYLVDWIRP
Length:170
Mass (Da):19,693
Last modified:July 1, 1997 - v1
Checksum:i41FA9B6959447E0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001577 Genomic DNA Translation: AAC45445.1
AE000666 Genomic DNA Translation: AAB84756.1
PIRiD69131

Genome annotation databases

EnsemblBacteriaiAAB84756; AAB84756; MTH_250
KEGGimth:MTH_250
PATRICifig|187420.15.peg.219

Similar proteinsi

Entry informationi

Entry nameiENDA_METTH
AccessioniPrimary (citable) accession number: O07165
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health