O07165 (ENDA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	MTH_250

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01833
Subunit structure	Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	tRNA-splicing endonuclease HAMAP-Rule MF_01833		PRO_0000109473
Sites
	Active site	106	1	By similarity
	Active site	116	1	By similarity
	Active site	147	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O07165 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 41FA9B6959447E0A	FASTA	170	19,693
	10 20 30 40 50 60 MRVEGQLGDE VVTIKATSIA RRLHGKSHYG KMYEDRLQLS LIEAAYLMER GKLKLMKDDD 70 80 90 100 110 120 EVSPEEFISL LGERGLYSKY LVYRDLRNRG YIVKTGFKYG AEFRLYERGG APGRTHSAYL 130 140 150 160 170 VRVISENDTI HALDFSSYVR VAHGVNKKLL MAFLDDEEDI TYYLVDWIRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems." Kleman-Leyer K., Armbruster D.W., Daniels C.J. Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF001577 Genomic DNA. Translation: AAC45445.1. AE000666 Genomic DNA. Translation: AAB84756.1.
PIR	D69131.
RefSeq	NP_275393.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O07165.
ModBase	Search...
Protein-protein interaction databases
IntAct	O07165. 1 interaction.
STRING	187420.MTH250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB84756; AAB84756; MTH_250.
GeneID	1470211.
KEGG	mth:MTH250.
Phylogenomic databases
eggNOG	COG1676.
KO	K01170.
OMA	FVRVAHS.
ProtClustDB	PRK09297.
Enzyme and pathway databases
BioCyc	MTHE187420:GJNM-250-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
HAMAP	MF_01833. EndA_short.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR016442. tRNA_splic_arch_short. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit. SSF55267. tRNA_int_endo_N. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_METTH

Accession

Primary (citable) accession number: O07165

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program