Reviewed, UniProtKB/Swiss-Prot O07137 (ECHA8_MYCLE)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA8
    EC=4.2.1.17

Gene names

Name:	echA8
Ordered Locus Names:	ML2402
ORF Names:	MLCB1306.05c

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable enoyl-CoA hydratase echA8		PRO_0000109337

Sequences

	Sequence		Length	Mass (Da)
	O07137-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 25B801EB690BCD00	FASTA	257	27,517
	10 20 30 40 50 60 MKYDTILVDG YQRVGIITLN RPQALNALNS QMMNEITNAA KELDIDPDVG AILITGSPKV 70 80 90 100 110 120 FAAGADIKEM ASLTFTDAFD ADFFSAWGKL AAVRTPMIAA VAGYALGGGC ELAMMCDLLI 130 140 150 160 170 180 AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTIDAAEA ERSGLVSRVV 190 200 210 220 230 240 LADDLLPEAK AVATTISQMS RSATRMAKEA VNRSFESTLA EGLLHERRLF HSTFVTDDQS 250 EGMAAFIEKR APQFTHR

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13803 Genomic DNA. Translation: CAA74130.1. AL583925 Genomic DNA. Translation: CAC31918.1.
PIR	F87209.
RefSeq	NP_302555.1.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ModBase	Search...
Genome annotation databases
GeneID	908604.
GenomeReviews	Gene locus ML2402 in contig AL450380_GR.
KEGG	mle:ML2402.
NMPDR	fig|272631.1.peg.1427.
Organism-specific databases
Leproma	ML2402.
CMR	Search...
Phylogenomic databases
HOGENOM	O07137.
OMA	LELAMIC.
Enzyme and pathway databases
BioCyc	MLEP272631:ML2402-MON.
BRENDA	4.2.1.17. 808.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHA8_MYCLE

Accession

Primary (citable) accession number: O07137

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)