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O07118

- ENDA_HALVD

UniProt

O07118 - ENDA_HALVD

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.3 Publications

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.1 PublicationUniRule annotation

    Cofactori

    Divalent cations; Ca2+ better than Mg2+.1 Publication

    pH dependencei

    Optimum pH is 6.5-8.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741UniRule annotation
    Active sitei285 – 2851UniRule annotation
    Active sitei316 – 3161UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-2958-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:HVO_2952
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339tRNA-splicing endonucleasePRO_0000109486Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    Homodimer.1 PublicationUniRule annotation

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000226561.
    KOiK01170.
    OMAiRGFYLSP.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O07118-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGRLEDGVV HLPGDARQRF HDSRGYGRPT GGDDLEVAPV EAAHLLSRDD    50
    IDGVDGMGLR ELLARTGTTL DFVVYKDLRD RGFYLSPARE GWPGVADAAD 100
    ADFLVYPRGK GPWDGEVEHR VRVVGERESI PVSSLGEVVL AIVDEDGDLT 150
    YFDTEGDDPE GTAAEDLPAD LDAELLSDRA LVWDGVDRLY QRGFFGQRLY 200
    GRNADSGPLQ LSLLEAAYLA RADALAIDEA DVVSRGRDVE GDRFDRRLAV 250
    YAALREAKTV PKSGFKFGSD FRVYTEFESV DDLSHSEFLV RVVAPDHTFV 300
    PRDLSLDVRL AGGVRKRMVF ALTDDNGEID WLSVSRLTP 339
    Length:339
    Mass (Da):37,418
    Last modified:September 21, 2011 - v2
    Checksum:i40825AEC6EB798E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511Y → I in AAC45446. (PubMed:9200602)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001578 Genomic DNA. Translation: AAC45446.1.
    CP001956 Genomic DNA. Translation: ADE04265.1.
    PIRiT44993.
    RefSeqiYP_003536957.1. NC_013967.1.

    Genome annotation databases

    EnsemblBacteriaiADE04265; ADE04265; HVO_2952.
    GeneIDi8926183.
    KEGGihvo:HVO_2952.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001578 Genomic DNA. Translation: AAC45446.1 .
    CP001956 Genomic DNA. Translation: ADE04265.1 .
    PIRi T44993.
    RefSeqi YP_003536957.1. NC_013967.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE04265 ; ADE04265 ; HVO_2952 .
    GeneIDi 8926183.
    KEGGi hvo:HVO_2952.

    Phylogenomic databases

    HOGENOMi HOG000226561.
    KOi K01170.
    OMAi RGFYLSP.

    Enzyme and pathway databases

    BioCyci HVOL309800:GCOK-2958-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems."
      Kleman-Leyer K., Armbruster D.W., Daniels C.J.
      Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 68-76; 90-107; 203-220 AND 273-283, FUNCTION, SUBUNIT.
      Strain: DS2 / DSM 5716 / WFD11.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    3. "A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties."
      Thompson L.D., Daniels C.J.
      J. Biol. Chem. 263:17951-17959(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE RECOGNITION.
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    4. "Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease."
      Thompson L.D., Daniels C.J.
      J. Biol. Chem. 265:18104-18111(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE RECOGNITION.
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

    Entry informationi

    Entry nameiENDA_HALVD
    AccessioniPrimary (citable) accession number: O07118
    Secondary accession number(s): D4GY02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3