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O07118

- ENDA_HALVD

UniProt

O07118 - ENDA_HALVD

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Protein

tRNA-splicing endonuclease

Gene
endA, HVO_2952
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.3 Publications

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.1 Publication

Cofactori

Divalent cations; Ca2+ better than Mg2+.1 Publication

pH dependencei

Optimum pH is 6.5-8.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741 By similarity
Active sitei285 – 2851 By similarity
Active sitei316 – 3161 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-2958-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:HVO_2952
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339tRNA-splicing endonucleaseUniRule annotationPRO_0000109486Add
BLAST

Post-translational modificationi

The N-terminus is blocked.UniRule annotation

Interactioni

Subunit structurei

Homodimer.1 Publication

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000226561.
KOiK01170.
OMAiRGFYLSP.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

O07118-1 [UniParc]FASTAAdd to Basket

« Hide

MQGRLEDGVV HLPGDARQRF HDSRGYGRPT GGDDLEVAPV EAAHLLSRDD    50
IDGVDGMGLR ELLARTGTTL DFVVYKDLRD RGFYLSPARE GWPGVADAAD 100
ADFLVYPRGK GPWDGEVEHR VRVVGERESI PVSSLGEVVL AIVDEDGDLT 150
YFDTEGDDPE GTAAEDLPAD LDAELLSDRA LVWDGVDRLY QRGFFGQRLY 200
GRNADSGPLQ LSLLEAAYLA RADALAIDEA DVVSRGRDVE GDRFDRRLAV 250
YAALREAKTV PKSGFKFGSD FRVYTEFESV DDLSHSEFLV RVVAPDHTFV 300
PRDLSLDVRL AGGVRKRMVF ALTDDNGEID WLSVSRLTP 339
Length:339
Mass (Da):37,418
Last modified:September 21, 2011 - v2
Checksum:i40825AEC6EB798E0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511Y → I in AAC45446. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001578 Genomic DNA. Translation: AAC45446.1.
CP001956 Genomic DNA. Translation: ADE04265.1.
PIRiT44993.
RefSeqiYP_003536957.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE04265; ADE04265; HVO_2952.
GeneIDi8926183.
KEGGihvo:HVO_2952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001578 Genomic DNA. Translation: AAC45446.1 .
CP001956 Genomic DNA. Translation: ADE04265.1 .
PIRi T44993.
RefSeqi YP_003536957.1. NC_013967.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE04265 ; ADE04265 ; HVO_2952 .
GeneIDi 8926183.
KEGGi hvo:HVO_2952.

Phylogenomic databases

HOGENOMi HOG000226561.
KOi K01170.
OMAi RGFYLSP.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-2958-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems."
    Kleman-Leyer K., Armbruster D.W., Daniels C.J.
    Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 68-76; 90-107; 203-220 AND 273-283, FUNCTION, SUBUNIT.
    Strain: DS2 / DSM 5716 / WFD11.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties."
    Thompson L.D., Daniels C.J.
    J. Biol. Chem. 263:17951-17959(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE RECOGNITION.
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  4. "Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease."
    Thompson L.D., Daniels C.J.
    J. Biol. Chem. 265:18104-18111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE RECOGNITION.
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

Entry informationi

Entry nameiENDA_HALVD
AccessioniPrimary (citable) accession number: O07118
Secondary accession number(s): D4GY02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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