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O07118

- ENDA_HALVD

UniProt

O07118 - ENDA_HALVD

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.3 Publications

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.1 PublicationUniRule annotation

Cofactori

Divalent cations; Ca2+ better than Mg2+.1 Publication

pH dependencei

Optimum pH is 6.5-8.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741UniRule annotation
Active sitei285 – 2851UniRule annotation
Active sitei316 – 3161UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-2958-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:HVO_2952
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339tRNA-splicing endonucleasePRO_0000109486Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Homodimer.1 PublicationUniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226561.
KOiK01170.
OMAiRGFYLSP.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

O07118-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGRLEDGVV HLPGDARQRF HDSRGYGRPT GGDDLEVAPV EAAHLLSRDD
60 70 80 90 100
IDGVDGMGLR ELLARTGTTL DFVVYKDLRD RGFYLSPARE GWPGVADAAD
110 120 130 140 150
ADFLVYPRGK GPWDGEVEHR VRVVGERESI PVSSLGEVVL AIVDEDGDLT
160 170 180 190 200
YFDTEGDDPE GTAAEDLPAD LDAELLSDRA LVWDGVDRLY QRGFFGQRLY
210 220 230 240 250
GRNADSGPLQ LSLLEAAYLA RADALAIDEA DVVSRGRDVE GDRFDRRLAV
260 270 280 290 300
YAALREAKTV PKSGFKFGSD FRVYTEFESV DDLSHSEFLV RVVAPDHTFV
310 320 330
PRDLSLDVRL AGGVRKRMVF ALTDDNGEID WLSVSRLTP
Length:339
Mass (Da):37,418
Last modified:September 21, 2011 - v2
Checksum:i40825AEC6EB798E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511Y → I in AAC45446. (PubMed:9200602)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001578 Genomic DNA. Translation: AAC45446.1.
CP001956 Genomic DNA. Translation: ADE04265.1.
PIRiT44993.
RefSeqiWP_004044805.1. NZ_AOHU01000104.1.
YP_003536957.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE04265; ADE04265; HVO_2952.
GeneIDi8926183.
KEGGihvo:HVO_2952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001578 Genomic DNA. Translation: AAC45446.1 .
CP001956 Genomic DNA. Translation: ADE04265.1 .
PIRi T44993.
RefSeqi WP_004044805.1. NZ_AOHU01000104.1.
YP_003536957.1. NC_013967.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE04265 ; ADE04265 ; HVO_2952 .
GeneIDi 8926183.
KEGGi hvo:HVO_2952.

Phylogenomic databases

HOGENOMi HOG000226561.
KOi K01170.
OMAi RGFYLSP.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-2958-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Properties of H. volcanii tRNA intron endonuclease reveal a relationship between the archaeal and eucaryal tRNA intron processing systems."
    Kleman-Leyer K., Armbruster D.W., Daniels C.J.
    Cell 89:839-847(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 68-76; 90-107; 203-220 AND 273-283, FUNCTION, SUBUNIT.
    Strain: DS2 / DSM 5716 / WFD11.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties."
    Thompson L.D., Daniels C.J.
    J. Biol. Chem. 263:17951-17959(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE RECOGNITION.
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  4. "Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease."
    Thompson L.D., Daniels C.J.
    J. Biol. Chem. 265:18104-18111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE RECOGNITION.
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

Entry informationi

Entry nameiENDA_HALVD
AccessioniPrimary (citable) accession number: O07118
Secondary accession number(s): D4GY02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3