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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene

ganB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the beta-1,4-galactan linkages of arabinogalactan type I, a pectic substance found in plants such as soybeans.1 Publication

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei194 – 1941Proton donorBy similarity
Binding sitei267 – 2671SubstrateBy similarity
Active sitei292 – 2921NucleophileBy similarity
Binding sitei296 – 2961SubstrateBy similarity
Metal bindingi303 – 3031CalciumBy similarity
Metal bindingi305 – 3051CalciumBy similarity
Metal bindingi307 – 3071CalciumBy similarity
Binding sitei311 – 3111SubstrateBy similarity
Binding sitei388 – 3881SubstrateBy similarity
Metal bindingi396 – 3961CalciumBy similarity
Metal bindingi399 – 3991CalciumBy similarity

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU34120-MONOMER.
MetaCyc:BSU34120-MONOMER.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase
Short name:
Galactanase
Gene namesi
Name:ganB
Synonyms:galA, yvfO
Ordered Locus Names:BSU34120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU34120. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121PROSITE-ProRule annotationAdd
BLAST
Chaini22 – 429408Arabinogalactan endo-beta-1,4-galactanasePRO_0000012222Add
BLAST

Proteomic databases

PaxDbiO07013.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU34120.

Structurei

3D structure databases

ProteinModelPortaliO07013.
SMRiO07013. Positions 41-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni146 – 1494Substrate bindingBy similarity
Regioni233 – 2342Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 53 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3867.
HOGENOMiHOG000118034.
InParanoidiO07013.
KOiK01224.
OMAiRWWFDEI.
OrthoDBiEOG6H4K5M.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O07013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKVKMFFA AAIVWSACSS TGYAAAIEKE KHVSELRAED LFVKKVEGMN
60 70 80 90 100
KDFIKGADVS SVIALENSGV TFYNTNGKRQ DIFTTLKQAG VNYVRVRIWN
110 120 130 140 150
HPYDSNGNGY GGGNNDVQKA IEIGKRATAN GMKVLADFHY SDFWADPAKQ
160 170 180 190 200
KVPKAWANLS FEAKKAKLYE YTKQSLQKMI KEGVDIGMVQ VGNETTGGFA
210 220 230 240 250
GETDWTKMCQ LFNEGSRAVR ETNSNILVAL HFTNPETAGR YSFIAETLSK
260 270 280 290 300
NKVDYDVFAS SYYPFWHGTL QNLTSVLKAV ANTYGKKVMV AETSYTYTAE
310 320 330 340 350
DGDGHGNTAP KSGQTLPYPI SVQGQATAVR DVMEAVANTG KAGLGVFYWE
360 370 380 390 400
PAWIPVGPKT QIEKNKVLWE TYGSGWASSY AAEYDPEDAG KWYGGSAVDN
410 420
QALFDFNGHP LPSLQVFQYA ESGHIPKKR
Length:429
Mass (Da):47,279
Last modified:July 1, 1997 - v1
Checksum:i8A32869EA42127AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z94043 Genomic DNA. Translation: CAB08009.1.
AL009126 Genomic DNA. Translation: CAB15417.2.
PIRiF70038.
RefSeqiNP_391292.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15417; CAB15417; BSU34120.
GeneIDi937086.
KEGGibsu:BSU34120.
PATRICi18978826. VBIBacSub10457_3576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z94043 Genomic DNA. Translation: CAB08009.1.
AL009126 Genomic DNA. Translation: CAB15417.2.
PIRiF70038.
RefSeqiNP_391292.2. NC_000964.3.

3D structure databases

ProteinModelPortaliO07013.
SMRiO07013. Positions 41-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU34120.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Proteomic databases

PaxDbiO07013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15417; CAB15417; BSU34120.
GeneIDi937086.
KEGGibsu:BSU34120.
PATRICi18978826. VBIBacSub10457_3576.

Organism-specific databases

GenoListiBSU34120. [Micado]

Phylogenomic databases

eggNOGiCOG3867.
HOGENOMiHOG000118034.
InParanoidiO07013.
KOiK01224.
OMAiRWWFDEI.
OrthoDBiEOG6H4K5M.

Enzyme and pathway databases

BioCyciBSUB:BSU34120-MONOMER.
MetaCyc:BSU34120-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR."
    Daniel R.A., Haiech J., Denizot F., Errington J.
    J. Bacteriol. 179:5636-5638(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. Denizot F.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases."
    Shipkowski S., Brenchley J.E.
    Appl. Environ. Microbiol. 72:7730-7738(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GALACTAN DEGRADATION.

Entry informationi

Entry nameiGANA_BACSU
AccessioniPrimary (citable) accession number: O07013
Secondary accession number(s): O32260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.