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O07013

- GANA_BACSU

UniProt

O07013 - GANA_BACSU

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Protein

Arabinogalactan endo-beta-1,4-galactanase

Gene
ganB, galA, yvfO, BSU34120
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the beta-1,4-galactan linkages of arabinogalactan type I, a pectic substance found in plants such as soybeans.1 Publication

Catalytic activityi

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei194 – 1941Proton donor By similarity
Binding sitei267 – 2671Substrate By similarity
Active sitei292 – 2921Nucleophile By similarity
Binding sitei296 – 2961Substrate By similarity
Metal bindingi303 – 3031Calcium By similarity
Metal bindingi305 – 3051Calcium By similarity
Metal bindingi307 – 3071Calcium By similarity
Binding sitei311 – 3111Substrate By similarity
Binding sitei388 – 3881Substrate By similarity
Metal bindingi396 – 3961Calcium By similarity
Metal bindingi399 – 3991Calcium By similarity

GO - Molecular functioni

  1. arabinogalactan endo-1,4-beta-galactosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU34120-MONOMER.
MetaCyc:BSU34120-MONOMER.

Protein family/group databases

CAZyiGH53. Glycoside Hydrolase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinogalactan endo-beta-1,4-galactanase (EC:3.2.1.89)
Alternative name(s):
Endo-1,4-beta-galactanase
Short name:
Galactanase
Gene namesi
Name:ganB
Synonyms:galA, yvfO
Ordered Locus Names:BSU34120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU34120. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 429408Arabinogalactan endo-beta-1,4-galactanasePRO_0000012222Add
BLAST

Proteomic databases

PaxDbiO07013.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU34120.

Structurei

3D structure databases

ProteinModelPortaliO07013.
SMRiO07013. Positions 41-423.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni146 – 1494Substrate binding By similarity
Regioni233 – 2342Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3867.
HOGENOMiHOG000118034.
KOiK01224.
OMAiIRLRLWV.
OrthoDBiEOG6H4K5M.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF07745. Glyco_hydro_53. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O07013-1 [UniParc]FASTAAdd to Basket

« Hide

MKSKVKMFFA AAIVWSACSS TGYAAAIEKE KHVSELRAED LFVKKVEGMN    50
KDFIKGADVS SVIALENSGV TFYNTNGKRQ DIFTTLKQAG VNYVRVRIWN 100
HPYDSNGNGY GGGNNDVQKA IEIGKRATAN GMKVLADFHY SDFWADPAKQ 150
KVPKAWANLS FEAKKAKLYE YTKQSLQKMI KEGVDIGMVQ VGNETTGGFA 200
GETDWTKMCQ LFNEGSRAVR ETNSNILVAL HFTNPETAGR YSFIAETLSK 250
NKVDYDVFAS SYYPFWHGTL QNLTSVLKAV ANTYGKKVMV AETSYTYTAE 300
DGDGHGNTAP KSGQTLPYPI SVQGQATAVR DVMEAVANTG KAGLGVFYWE 350
PAWIPVGPKT QIEKNKVLWE TYGSGWASSY AAEYDPEDAG KWYGGSAVDN 400
QALFDFNGHP LPSLQVFQYA ESGHIPKKR 429
Length:429
Mass (Da):47,279
Last modified:July 1, 1997 - v1
Checksum:i8A32869EA42127AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z94043 Genomic DNA. Translation: CAB08009.1.
AL009126 Genomic DNA. Translation: CAB15417.2.
PIRiF70038.
RefSeqiNP_391292.2. NC_000964.3.
WP_003243128.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB15417; CAB15417; BSU34120.
GeneIDi937086.
KEGGibsu:BSU34120.
PATRICi18978826. VBIBacSub10457_3576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z94043 Genomic DNA. Translation: CAB08009.1 .
AL009126 Genomic DNA. Translation: CAB15417.2 .
PIRi F70038.
RefSeqi NP_391292.2. NC_000964.3.
WP_003243128.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali O07013.
SMRi O07013. Positions 41-423.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU34120.

Protein family/group databases

CAZyi GH53. Glycoside Hydrolase Family 53.

Proteomic databases

PaxDbi O07013.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15417 ; CAB15417 ; BSU34120 .
GeneIDi 937086.
KEGGi bsu:BSU34120.
PATRICi 18978826. VBIBacSub10457_3576.

Organism-specific databases

GenoListi BSU34120. [Micado ]

Phylogenomic databases

eggNOGi COG3867.
HOGENOMi HOG000118034.
KOi K01224.
OMAi IRLRLWV.
OrthoDBi EOG6H4K5M.

Enzyme and pathway databases

BioCyci BSUB:BSU34120-MONOMER.
MetaCyc:BSU34120-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR011683. Glyco_hydro_53.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF07745. Glyco_hydro_53. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR."
    Daniel R.A., Haiech J., Denizot F., Errington J.
    J. Bacteriol. 179:5636-5638(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. Denizot F.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases."
    Shipkowski S., Brenchley J.E.
    Appl. Environ. Microbiol. 72:7730-7738(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GALACTAN DEGRADATION.

Entry informationi

Entry nameiGANA_BACSU
AccessioniPrimary (citable) accession number: O07013
Secondary accession number(s): O32260
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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