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Reviewed, UniProtKB/Swiss-Prot O07003 (LEVB_BACSU)

Last modified June 15, 2010. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Levanbiose-producing levanase
EC=3.2.1.64
Alternative name(s):
2,6-beta-fructan 6-levanbiohydrolase
Endo-levanase
Gene names
Name:levB
Synonyms:yveB
Ordered Locus Names:BSU34460
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus
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Protein attributesHide

Sequence length516 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Catalyzes the degradation of levan mainly into levanbiose (difructose). Is not active on sucrose. Ref.3 Ref.4

Catalytic activity

Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain.

Subcellular location

Cell membrane; Single-pass membrane protein Ref.3.

Induction

Induced by sucrose. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

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OntologiesHide

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function2,6-beta-fructan 6-levanbiohydrolase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 516516Levanbiose-producing levanase
PRO_0000344254

Regions

Topological domain1 – 55Cytoplasmic Potential
Transmembrane6 – 2621Helical; Potential
Topological domain27 – 516490Extracellular Potential
Region55 – 584Substrate binding By similarity
Region116 – 1172Substrate binding By similarity
Region181 – 1822Substrate binding By similarity

Sites

Active site581 By similarity
Binding site2301Substrate By similarity
Binding site3181Substrate By similarity
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SequencesHide

Sequence LengthMass (Da)Tools
O07003-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: EAEC526706C40762

FASTA51658,979
        10         20         30         40         50         60 
MNYIKAGKWL TVFLTFLGIL LFIDLFPKEE HDQKTKSKQK PDYRAAYHFT TPDKWKNDPQ 

        70         80         90        100        110        120 
KPIYFDGKYH YFYLYNRDYP KGNGTEWRHA VSEDLVHWTD EGVAIPKYTN PDGDIWTGSV 

       130        140        150        160        170        180 
VVDKENTAGF GKNALVAIVT QPSAKDKKQE QYLWYSTDKG KSFKFYSGNP VMPNPGTDDF 

       190        200        210        220        230        240 
RDPKVIWDDQ DNKWVMVMAE GSKIGFYESD NLKDWHYTSG FFPEQAGMVE CPDLYMMRAS 

       250        260        270        280        290        300 
DGTNKWVLGA SANGKPWGKP NTYAYWTGSF DGKEFKADQT EAQWLDYGFD WYGGVTFEDS 

       310        320        330        340        350        360 
KSTDPLEKRY ALAWMNNWDY ANNTPTMKNG FNGTDSVIRE LRLKEQDGTY SLVSQPIEAL 

       370        380        390        400        410        420 
EQLTVSTDEI EDQDVNGSKT LSITGDTYQL DTDLSWSELK NAGVRLRESE DQKRHIDVGI 

       430        440        450        460        470        480 
FAEGGYAYVN RAATNQPDKS NTYVESKAPY DVNKRKVHLK ILVDKTTIEV FVGDGKTVFS 

       490        500        510 
NEVFPKPEDK GITLYSDGGT ASFKNITVKH FDSIHE

« Hide

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ReferencesHide

« Hide 'large scale' references
[1]Denizot F.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"yveB, encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis."
Pereira Y., Petit-Glatron M.-F., Chambert R.
Microbiology 147:3413-3419(2001) [PubMed: 11739774] [Abstract]
Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION.
[4]"Autogenous modulation of the Bacillus subtilis sacB-levB-yveA levansucrase operon by the levB transcript."
Daguer J.-P., Geissmann T., Petit-Glatron M.-F., Chambert R.
Microbiology 150:3669-3679(2004) [PubMed: 15528654] [Abstract]
Cited for: FUNCTION AS A LEVANBIOSE-PRODUCING LEVANASE.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ		Z94043 Genomic DNA. Translation: CAB08014.1.
AL009126 Genomic DNA. Translation: CAB15451.1.
PIRE70035.
RefSeqNP_391326.1.

3D structure databases

HSSPHSSP built from PDB template 1UYP based on UniProtKB O33833.
SMRO07003. Positions 41-514.
ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Genome annotation databases

EnsemblBacteriaEBBACT00000000473; EBBACP00000000473; EBBACG00000000471.
GeneID938616.
GenomeReviewsGene locus BSU34460 in contig AL009126_GR.
KEGGbsu:BSU34460.
NMPDRfig|224308.1.peg.3452.

Organism-specific databases

GenoListBSU34460. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMHBG693902.
OMATYAYWTG.
PhylomeDBO07003.
ProtClustDBCLSK882933.

Enzyme and pathway databases

BioCycSUBTI:BSU34460-MONOMER.

Family and domain databases

InterProIPR008985. ConA-like_lec_gl.
IPR001362. Glyco_hydro_32.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. False negative.
[Graphical view]
ProtoNetSearch...
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Entry informationHide

Entry nameLEVB_BACSU
AccessionPrimary (citable) accession number: O07003
Secondary accession number(s): Q795H7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 1, 1997
Last modified: June 15, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents