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Protein

Oligo-1,6-glucosidase 1

Gene

malL

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes various disaccharides such as sucrose, maltose, and isomaltose with different efficiencies. Also hydrolyzes longer maltodextrins from maltotriose up to maltohexaose, but not maltoheptaose, palatinose, isomaltotriose, or isomaltotetraose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.3 Publications

Temperature dependencei

Optimum temperature is 42 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20CalciumCombined sources1 Publication1
Metal bindingi22CalciumCombined sources1 Publication1
Metal bindingi24CalciumCombined sources1 Publication1
Metal bindingi26Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi28CalciumCombined sources1 Publication1
Active sitei199NucleophileBy similarity1
Active sitei255Proton donorBy similarity1
Sitei332Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU34560-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase 1 (EC:3.2.1.103 Publications)
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase 1
Sucrase-isomaltase 1
Short name:
Isomaltase 1
Gene namesi
Name:malL
Synonyms:yvdL
Ordered Locus Names:BSU34560
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543171 – 561Oligo-1,6-glucosidase 1Add BLAST561

Proteomic databases

PaxDbiO06994.
PRIDEiO06994.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018721.

Structurei

Secondary structure

1561
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Beta strandi10 – 13Combined sources4
Helixi15 – 17Combined sources3
Beta strandi21 – 26Combined sources6
Helixi29 – 42Combined sources14
Beta strandi46 – 49Combined sources4
Turni58 – 61Combined sources4
Beta strandi65 – 70Combined sources6
Turni72 – 74Combined sources3
Helixi77 – 89Combined sources13
Beta strandi93 – 98Combined sources6
Helixi108 – 113Combined sources6
Helixi122 – 124Combined sources3
Beta strandi145 – 152Combined sources8
Turni153 – 156Combined sources4
Beta strandi157 – 160Combined sources4
Helixi175 – 190Combined sources16
Beta strandi195 – 198Combined sources4
Helixi201 – 203Combined sources3
Beta strandi220 – 222Combined sources3
Helixi225 – 227Combined sources3
Helixi233 – 243Combined sources11
Helixi245 – 247Combined sources3
Beta strandi251 – 255Combined sources5
Helixi261 – 268Combined sources8
Helixi270 – 272Combined sources3
Beta strandi276 – 279Combined sources4
Turni282 – 284Combined sources3
Helixi295 – 297Combined sources3
Helixi304 – 317Combined sources14
Beta strandi319 – 322Combined sources4
Beta strandi324 – 326Combined sources3
Helixi336 – 340Combined sources5
Helixi347 – 359Combined sources13
Beta strandi361 – 368Combined sources8
Helixi371 – 373Combined sources3
Helixi382 – 384Combined sources3
Helixi388 – 397Combined sources10
Turni398 – 400Combined sources3
Helixi406 – 416Combined sources11
Helixi418 – 421Combined sources4
Beta strandi428 – 430Combined sources3
Helixi431 – 434Combined sources4
Turni446 – 450Combined sources5
Helixi453 – 458Combined sources6
Helixi463 – 476Combined sources14
Helixi479 – 482Combined sources4
Beta strandi486 – 489Combined sources4
Beta strandi493 – 502Combined sources10
Beta strandi505 – 512Combined sources8
Beta strandi514 – 516Combined sources3
Beta strandi518 – 521Combined sources4
Helixi524 – 526Combined sources3
Beta strandi531 – 538Combined sources8
Beta strandi547 – 550Combined sources4
Beta strandi555 – 560Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M56X-ray2.30A/B1-561[»]
4M8UX-ray1.45A1-561[»]
4MAZX-ray1.60A1-561[»]
4MB1X-ray1.40A1-561[»]
ProteinModelPortaliO06994.
SMRiO06994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000220641.
InParanoidiO06994.
KOiK01182.
OMAiQFEHITL.
PhylomeDBiO06994.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032091. Malt_amylase_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF16657. Malt_amylase_C. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O06994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEWWKEAVV YQIYPRSFYD ANGDGFGDLQ GVIQKLDYIK NLGADVIWLS
60 70 80 90 100
PVFDSPQDDN GYDISDYKNM YEKFGTNEDM FQLIDEVHKR GMKIVMDLVV
110 120 130 140 150
NHTSDEHAWF AESRKSKDNP YRDYYLWKDP KPDGSEPNNW GSIFSGSAWT
160 170 180 190 200
YDEGTGQYYL HYFSKKQPDL NWENEAVRRE VYDVMRFWMD RGVDGWRMDV
210 220 230 240 250
IGSISKYTDF PDYETDHSRS YIVGRYHSNG PRLHEFIQEM NREVLSHYDC
260 270 280 290 300
MTVGEANGSD IEEAKKYTDA SRQELNMIFT FEHMDIDKEQ NSPNGKWQIK
310 320 330 340 350
PFDLIALKKT MTRWQTGLMN VGWNTLYFEN HDQPRVISRW GNDRKLRKEC
360 370 380 390 400
AKAFATVLHG MKGTPFIYQG EEIGMVNSDM PLEMYDDLEI KNAYRELVVE
410 420 430 440 450
NKTMSEKEFV KAVMIKGRDH ARTPMQWDAG KHAGFTAGDP WIPVNSRYQD
460 470 480 490 500
INVKESLEDQ DSIFFYYQKL IQLRKQYKIM IYGDYQLLQE NDPQVFSYLR
510 520 530 540 550
EYRGEKLLVV VNLSEEKALF EAPPELIHER WKVLISNYPQ ERADLKSISL
560
KPYEAVMGIS I
Length:561
Mass (Da):66,081
Last modified:July 1, 1997 - v1
Checksum:i2B43E9A4AD65A222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z94043 Genomic DNA. Translation: CAB08041.1.
AL009126 Genomic DNA. Translation: CAB15461.1.
PIRiD70034.
RefSeqiNP_391336.1. NC_000964.3.
WP_003243333.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15461; CAB15461; BSU34560.
GeneIDi938623.
KEGGibsu:BSU34560.
PATRICi18978916. VBIBacSub10457_3620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z94043 Genomic DNA. Translation: CAB08041.1.
AL009126 Genomic DNA. Translation: CAB15461.1.
PIRiD70034.
RefSeqiNP_391336.1. NC_000964.3.
WP_003243333.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M56X-ray2.30A/B1-561[»]
4M8UX-ray1.45A1-561[»]
4MAZX-ray1.60A1-561[»]
4MB1X-ray1.40A1-561[»]
ProteinModelPortaliO06994.
SMRiO06994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018721.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiO06994.
PRIDEiO06994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15461; CAB15461; BSU34560.
GeneIDi938623.
KEGGibsu:BSU34560.
PATRICi18978916. VBIBacSub10457_3620.

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000220641.
InParanoidiO06994.
KOiK01182.
OMAiQFEHITL.
PhylomeDBiO06994.

Enzyme and pathway databases

BioCyciBSUB:BSU34560-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032091. Malt_amylase_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF16657. Malt_amylase_C. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO16G1_BACSU
AccessioniPrimary (citable) accession number: O06994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.