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Protein

Oligo-1,6-glucosidase 1

Gene

malL

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes various disaccharides such as sucrose, maltose, and isomaltose with different efficiencies. Also hydrolyzes longer maltodextrins from maltotriose up to maltohexaose, but not maltoheptaose, palatinose, isomaltotriose, or isomaltotetraose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.3 Publications

Temperature dependencei

Optimum temperature is 42 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20CalciumCombined sources1 Publication1
Metal bindingi22CalciumCombined sources1 Publication1
Metal bindingi24CalciumCombined sources1 Publication1
Metal bindingi26Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi28CalciumCombined sources1 Publication1
Active sitei199NucleophileBy similarity1
Active sitei255Proton donorBy similarity1
Sitei332Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU34560-MONOMER

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase 1 (EC:3.2.1.103 Publications)
Alternative name(s):
Dextrin 6-alpha-D-glucanohydrolase
Oligosaccharide alpha-1,6-glucosidase 1
Sucrase-isomaltase 1
Short name:
Isomaltase 1
Gene namesi
Name:malL
Synonyms:yvdL
Ordered Locus Names:BSU34560
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543171 – 561Oligo-1,6-glucosidase 1Add BLAST561

Proteomic databases

PaxDbiO06994
PRIDEiO06994

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018721

Structurei

Secondary structure

1561
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Beta strandi10 – 13Combined sources4
Helixi15 – 17Combined sources3
Beta strandi21 – 26Combined sources6
Helixi29 – 42Combined sources14
Beta strandi46 – 49Combined sources4
Turni58 – 61Combined sources4
Beta strandi65 – 70Combined sources6
Turni72 – 74Combined sources3
Helixi77 – 89Combined sources13
Beta strandi93 – 98Combined sources6
Helixi108 – 113Combined sources6
Helixi122 – 124Combined sources3
Beta strandi145 – 152Combined sources8
Turni153 – 156Combined sources4
Beta strandi157 – 160Combined sources4
Helixi175 – 190Combined sources16
Beta strandi195 – 198Combined sources4
Helixi201 – 203Combined sources3
Beta strandi220 – 222Combined sources3
Helixi225 – 227Combined sources3
Helixi233 – 243Combined sources11
Helixi245 – 247Combined sources3
Beta strandi251 – 255Combined sources5
Helixi261 – 268Combined sources8
Helixi270 – 272Combined sources3
Beta strandi276 – 279Combined sources4
Turni282 – 284Combined sources3
Helixi295 – 297Combined sources3
Helixi304 – 317Combined sources14
Beta strandi319 – 322Combined sources4
Beta strandi324 – 326Combined sources3
Helixi336 – 340Combined sources5
Helixi347 – 359Combined sources13
Beta strandi361 – 368Combined sources8
Helixi371 – 373Combined sources3
Helixi382 – 384Combined sources3
Helixi388 – 397Combined sources10
Turni398 – 400Combined sources3
Helixi406 – 416Combined sources11
Helixi418 – 421Combined sources4
Beta strandi428 – 430Combined sources3
Helixi431 – 434Combined sources4
Turni446 – 450Combined sources5
Helixi453 – 458Combined sources6
Helixi463 – 476Combined sources14
Helixi479 – 482Combined sources4
Beta strandi486 – 489Combined sources4
Beta strandi493 – 502Combined sources10
Beta strandi505 – 512Combined sources8
Beta strandi514 – 516Combined sources3
Beta strandi518 – 521Combined sources4
Helixi524 – 526Combined sources3
Beta strandi531 – 538Combined sources8
Beta strandi547 – 550Combined sources4
Beta strandi555 – 560Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M56X-ray2.30A/B1-561[»]
4M8UX-ray1.45A1-561[»]
4MAZX-ray1.60A1-561[»]
4MB1X-ray1.40A1-561[»]
5WCZX-ray1.58A/B1-561[»]
ProteinModelPortaliO06994
SMRiO06994
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105CG3 Bacteria
COG0366 LUCA
HOGENOMiHOG000220641
InParanoidiO06994
KOiK01182
OMAiHEFIQEM
PhylomeDBiO06994

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR032091 Malt_amylase_C
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF16657 Malt_amylase_C, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

O06994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEWWKEAVV YQIYPRSFYD ANGDGFGDLQ GVIQKLDYIK NLGADVIWLS
60 70 80 90 100
PVFDSPQDDN GYDISDYKNM YEKFGTNEDM FQLIDEVHKR GMKIVMDLVV
110 120 130 140 150
NHTSDEHAWF AESRKSKDNP YRDYYLWKDP KPDGSEPNNW GSIFSGSAWT
160 170 180 190 200
YDEGTGQYYL HYFSKKQPDL NWENEAVRRE VYDVMRFWMD RGVDGWRMDV
210 220 230 240 250
IGSISKYTDF PDYETDHSRS YIVGRYHSNG PRLHEFIQEM NREVLSHYDC
260 270 280 290 300
MTVGEANGSD IEEAKKYTDA SRQELNMIFT FEHMDIDKEQ NSPNGKWQIK
310 320 330 340 350
PFDLIALKKT MTRWQTGLMN VGWNTLYFEN HDQPRVISRW GNDRKLRKEC
360 370 380 390 400
AKAFATVLHG MKGTPFIYQG EEIGMVNSDM PLEMYDDLEI KNAYRELVVE
410 420 430 440 450
NKTMSEKEFV KAVMIKGRDH ARTPMQWDAG KHAGFTAGDP WIPVNSRYQD
460 470 480 490 500
INVKESLEDQ DSIFFYYQKL IQLRKQYKIM IYGDYQLLQE NDPQVFSYLR
510 520 530 540 550
EYRGEKLLVV VNLSEEKALF EAPPELIHER WKVLISNYPQ ERADLKSISL
560
KPYEAVMGIS I
Length:561
Mass (Da):66,081
Last modified:July 1, 1997 - v1
Checksum:i2B43E9A4AD65A222
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z94043 Genomic DNA Translation: CAB08041.1
AL009126 Genomic DNA Translation: CAB15461.1
PIRiD70034
RefSeqiNP_391336.1, NC_000964.3
WP_003243333.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15461; CAB15461; BSU34560
GeneIDi938623
KEGGibsu:BSU34560
PATRICifig|224308.179.peg.3743

Entry informationi

Entry nameiO16G1_BACSU
AccessioniPrimary (citable) accession number: O06994
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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