Reviewed, UniProtKB/Swiss-Prot O06988 (BBMA1_BACSU)

Names and origin

Protein names

Recommended name:
    Intracellular maltogenic amylase
    EC=3.2.1.-

Gene names

Name:	bbmA
Synonyms:	yvdF
Ordered Locus Names:	BSU34620

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferring glucose or maltose to another moiety of sugars by forming alpha-(1,6)- and alpha-(1,3)-glycosidic linkages upon the hydrolysis of substrate at concentrations of 5% or higher By similarity.
Subunit structure	Monomer or homodimer; in equilibrium By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. BbmA subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Intracellular maltogenic amylase		PRO_0000366977
Sites
	Active site	327	1	Nucleophile By similarity
	Active site	356	1	Proton donor By similarity
	Active site	423	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O06988-1 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 6132681370919DFE	FASTA	588	68,722
	10 20 30 40 50 60 MEYAAIHHQP FSTDAYSYDG RTVHIKIRTK KGDADHIRFI WGDPYEYNDG KWSANEQPMR 70 80 90 100 110 120 KIAATEMHDY WFAEVVPPFR RLQYAFVVTD DHEDIFFGSS GVCPYNEKTL ETIHYYFKFP 130 140 150 160 170 180 FVHEADTFQA PEWVKSTVWY QIFPERFANG REDLSPKNAL PWGSKDPDVN DFFGGDLQGI 190 200 210 220 230 240 VDKLDYLEDL GVNGIYLTPI FSAPSNHKYD TLDYFSIDPH FGDPELFRTL VSQLHQRGMR 250 260 270 280 290 300 IMLDAVFNHI GSASPQWQDV VKNGDQSRYK DWFHIHSFPV TDDNYDRFAF TADMPKLNTA 310 320 330 340 350 360 NPEVQKYLLD IALYWIREFD IDGWRLDVAN EVDHVFWKTF RQAVSTEKPD VYILGEIWHS 370 380 390 400 410 420 AEPWLRGDEF HAAMNYPFTE PMIEYFADQT ISASRMAHRV NAHLMNGMKQ ANEVMFNLLD 430 440 450 460 470 480 SHDTKRLLTR CRNDEKKARA LLAFMFAQTG SPCIYYGTEI GLNGENDPLC RKCMVWEKEK 490 500 510 520 530 540 QNQDMLQFMK RLIALRKQEN TLLTEGHLEW NLLDDKNDFI SFSRTLDEKI LIYFFNQGNV 550 560 570 580 VQHVSLRELN IDRNKKICDA WTEQPLQHHD VIAVQPGEFL ILSAAAPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Denizot F. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z94043 Genomic DNA. Translation: CAB08035.1. Different initiation. AL009126 Genomic DNA. Translation: CAB15467.1. Different initiation.
PIR	F70033.
RefSeq	NP_391342.1.
3D structure databases
HSSP	HSSP built from PDB template 1J0H based on UniProtKB P38940.
ModBase	Search...
Protein family/group databases
CAZy	CBM34. Carbohydrate-Binding Module Family 34. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	936514.
GenomeReviews	Gene locus BSU34620 in contig AL009126_GR.
KEGG	bsu:BSU34620.
NMPDR	fig|224308.1.peg.3468.
Organism-specific databases
SubtiList	BG12415. bbmA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O06988.
OMA	O06988. VYILGEI.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3459-MON.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BBMA1_BACSU

Accession

Primary (citable) accession number: O06988
Secondary accession number(s): Q795G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)