Reviewed, UniProtKB/Swiss-Prot O06914 (FRDB_HELPY)

Names and origin

Protein names

Recommended name:
    Fumarate reductase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	frdB
Ordered Locus Names:	HP_0191

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur, and cytochrome b-556 By similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Fumarate reductase iron-sulfur subunit		PRO_0000158703
Regions
	Domain	17 – 98	82	2Fe-2S ferredoxin-type
	Domain	145 – 174	30	4Fe-4S ferredoxin-type
Sites
	Metal binding	60	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	68	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	80	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	154	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	160	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	164	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	211	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	217	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	221	1	Iron-sulfur 2 (4Fe-4S) By similarity
Experimental info
	Sequence conflict	140	1	V → I in AAC46065. Ref.1
	Sequence conflict	194	1	N → S in AAC46065. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O06914-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: D54447471FB4C499	FASTA	245	27,652
	10 20 30 40 50 60 MSDNERTIVV RVLKFDPQSA VSKPHFKEYQ LKETPSMTLF IALNLIREHQ DPDLSFDFVC 70 80 90 100 110 120 RAGICGSCAM MVNGRPRLAC KTLTSSFESG VITLMPMPSF TLIKDLSVNT GDWFLDMTKR 130 140 150 160 170 180 VESWAHSKEE VDITRPEKRV EPDEAQEVFE LDRCIECGCC IASCGTKLMR PNFIGAAGMN 190 200 210 220 230 240 RAMRFMIDSH DERNDDDFYE LVGDDDGVFG CMSLIACHDT CPKELPLQSS IATLRNRMLK VGKSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional characterization of Helicobacter pylori fumarate reductase operon comprising three structural genes coding for subunits C, A and B." Ge Z., Jiang Q., Kalisiak M.S., Taylor D.E. Gene 204:227-234(1997) [PubMed: 9434188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43629 / JCM 7656 / NCTC 11639 / UA802.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U78101 Genomic DNA. Translation: AAC46065.1. AE000511 Genomic DNA. Translation: AAD07258.1.
PIR	G64543.
RefSeq	NP_206990.1.
3D structure databases
HSSP	HSSP built from PDB template 1QLA based on UniProtKB P17596.
SMR	O06914. Positions 6-241.
ModBase	Search...
Genome annotation databases
GeneID	899155.
GenomeReviews	Gene locus HP_0191 in contig AE000511_GR.
KEGG	hpy:HP0191.
NMPDR	fig|85962.1.peg.188.
TIGR	HP_0191.
Phylogenomic databases
HOGENOM	O06914.
OMA	O06914. RVESWAH.
Enzyme and pathway databases
BRENDA	1.3.99.1. 1131.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRDB_HELPY

Accession

Primary (citable) accession number: O06914

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)