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O06901 (MALH_FUSMR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Maltose-6'-phosphate glucosidase

EC=3.2.1.122
Alternative name(s):
6-phospho-alpha-D-glucosidase
Gene names
Name:malH
OrganismFusobacterium mortiferum
Taxonomic identifier850 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'P, trehalose-6P and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'P, turanose-6'P, maltulose-6'P, leucrose-6'P, and palatinose-6'P. However, sucrose-6P is not a substrate for MalH, and this enzyme also fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'P and gentobiose-6'P.

Catalytic activity

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

Cofactor

NAD.

Divalent metal ion. Manganese, iron, cobalt and nickel ions enhance activity whereas magnesium, zinc, calcium and strontium do not.

Pathway

Glycan degradation; maltose degradation.

Subunit structure

Homotetramer By similarity.

Induction

By the five sucrose isomers and other alpha-glucosides (but not by sucrose or glucose).

Sequence similarities

Belongs to the glycosyl hydrolase 4 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0-7.5.

Temperature dependence:

Optimum temperature is 40 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Maltose-6'-phosphate glucosidase
PRO_0000169864

Regions

Nucleotide binding4 – 7067NAD By similarity

Sites

Active site1701Proton donor By similarity
Active site2641Proton acceptor By similarity
Metal binding1691Manganese By similarity
Metal binding2001Manganese By similarity
Binding site931Substrate By similarity
Binding site1471Substrate By similarity
Binding site2841Substrate By similarity
Site1091Increases basicity of active site Tyr By similarity

Sequences

Sequence LengthMass (Da)Tools
O06901 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: EE9D85B35FA8AF46

FASTA44149,712
        10         20         30         40         50         60 
MKQFSILIAG GGSTFTPGII LMLLDNLDKF PIRQIKMFDN DAERQAKIGE ACAILLKEKA 

        70         80         90        100        110        120 
PQIKFSYSTN PEEAFTDIDF VMAHIRVGKY PMRELDEKIP LRHGVVGQET CGPGGIAYGM 

       130        140        150        160        170        180 
RSIGGVIGLI DYMEKYSPNA WMLNYSNPAA IVAEATRRLR PNSKVLNICD MPIGIEVRMA 

       190        200        210        220        230        240 
EILGLESRKD MDIMYYGLNH FGWWKSVRDK QGNDLMPKLR EHVSQYGYVV PKGDNQHTEA 

       250        260        270        280        290        300 
SWNDTFAKAK DVLALDPTTL PNTYLKYYLF PDYVVEHSNK EYTRANEVMD GREKFVFGEC 

       310        320        330        340        350        360 
EKVVKNQSSE GCALHIDEHA SYIVDLARAI AFNTKEKMLL IVENNGAIVN FDSTAMVEIP 

       370        380        390        400        410        420 
CIVGSNGPEP LVVGRIPQFQ KGMMEQQVTV EKLTVEAWIE GSYQKLWQAI TMSKTVPSAK 

       430        440 
VAKDILDDLI EANKEYWPVL K 

« Hide

References

[1]"6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases."
Bouma C.L., Reizer J., Reizer A., Robrish S.A., Thompson J.
J. Bacteriol. 179:4129-4137(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25557 / CCUG 14475.
[2]"Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides."
Thompson J., Gentry-Weeks C.R., Nguyen N.Y., Folk J.E., Robrish S.A.
J. Bacteriol. 177:2505-2512(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-32, CHARACTERIZATION.
Strain: ATCC 25557 / CCUG 14475.
[3]"Metabolism of sucrose and its five isomers by Fusobacterium mortiferum."
Pikis A., Immel S., Robrish S.A., Thompson J.
Microbiology 148:843-852(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY.
Strain: ATCC 25557 / CCUG 14475.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U81185 Genomic DNA. Translation: AAB63015.1.

3D structure databases

ProteinModelPortalO06901.
SMRO06901. Positions 2-440.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.2.1.122. 2369.
SABIO-RKO06901.
UniPathwayUPA00150.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSPR00732. GLHYDRLASE4.
SUPFAMSSF56327. SSF56327. 1 hit.
PROSITEPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMALH_FUSMR
AccessionPrimary (citable) accession number: O06901
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries