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O06900 (PTUCB_FUSMR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system alpha-glucoside-specific EIICB component

Including the following 2 domains:

  1. Alpha-glucoside permease IIC component
    Alternative name(s):
    PTS system alpha-glucoside-specific EIIC component
  2. Alpha-glucoside-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system alpha-glucoside-specific EIIB component
Gene names
Name:malB
OrganismFusobacterium mortiferum
Taxonomic identifier850 [NCBI]
Taxonomic lineageBacteria › Fusobacteria › Fusobacteriales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in alpha-glucoside transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526PTS system alpha-glucoside-specific EIICB component
PRO_0000186477

Regions

Transmembrane12 – 3221Helical; Potential
Transmembrane59 – 7921Helical; Potential
Transmembrane88 – 10821Helical; Potential
Transmembrane132 – 15221Helical; Potential
Transmembrane173 – 19321Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane224 – 24421Helical; Potential
Transmembrane274 – 29421Helical; Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane330 – 35021Helical; Potential
Transmembrane355 – 37521Helical; Potential
Transmembrane381 – 40121Helical; Potential
Domain1 – 417417PTS EIIC type-1
Domain447 – 52680PTS EIIB type-1

Sites

Active site4691Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O06900 [UniParc].

Last modified October 1, 2001. Version 2.
Checksum: E5FDE7287202D84E

FASTA52657,313
        10         20         30         40         50         60 
MLKHFQRLGG ALFAPVLLFP FAGLVVALTI ILKNPDFVGE LANTNGTFYK MITVIEEGGW 

        70         80         90        100        110        120 
TVFRQLPLIF AIGLPIGLAK KAHPRACLAV LATYLTYNYF ISAILTFWGP SFGVDFTQNV 

       130        140        150        160        170        180 
GGVSGLTTIA GIKTLDTSIV GAIVISGITI YIHNKFFDTK LPDFLGTFQG TTLVSAIAFV 

       190        200        210        220        230        240 
VMIPCAYITC LVWPKIQMGI SSLQALMVTS GTFGVWLYTF LERILIPTGL HHFIYGPFIF 

       250        260        270        280        290        300 
GPAVVDTGIQ VAWAENLLNF ANSTQPLKEL FPQGGFALHG NSKIFGCIGI ALAMYKTARP 

       310        320        330        340        350        360 
EKKKIVSGLL IPAALTAALV GITEPLEFTF LFIAPFLFVV HAVLAATMAA VMYAFGVVKY 

       370        380        390        400        410        420 
GSGIIEIAAL NWLPLMKNHS GVMFTQLAIG VVFIGIHYLV FKFLIEKYNV KTSGREDEEE 

       430        440        450        460        470        480 
ETKLYTKADW KAKNGEGKET NSSDLYSGKA KAFLEAFGGK DNIEQVNNCA TRLRISVKDE 

       490        500        510        520 
KKVGPDIQFK AAGAHGVVRN GKAFQVIVGL SVPQVRESFE NLMEQN

« Hide

References

[1]"Metabolism of sucrose and its five isomers by Fusobacterium mortiferum."
Pikis A., Immel S., Robrish S.A., Thompson J.
Microbiology 148:843-852(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25557 / CCUG 14475.
[2]"6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases."
Bouma C.L., Reizer J., Reizer A., Robrish S.A., Thompson J.
J. Bacteriol. 179:4129-4137(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 443-526.
Strain: ATCC 25557 / CCUG 14475.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U81185 Genomic DNA. Translation: AAB63014.2.

3D structure databases

ProteinModelPortalO06900.
ModBaseSearch...

Protein family/group databases

TCDB4.A.1.1.4. PTS glucose-glucoside (Glc) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.1360.60. 1 hit.
InterProIPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR001996. PTS_EIIB_1.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR010975. PTS_IIBC_a_glc.
[Graphical view]
PfamPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMSSF55604. PTS_EIIB. 1 hit.
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR02005. PTS-IIBC-alpha. 1 hit.
PROSITEPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTUCB_FUSMR
AccessionPrimary (citable) accession number: O06900
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2001
Last modified: April 3, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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