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O06745 (YITJ_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase

Including the following 2 domains:

  1. Homocysteine S-methyltransferase
    EC=2.1.1.10
    Alternative name(s):
    S-methylmethionine:homocysteine methyltransferase
  2. 5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20
Gene names
Name:yitJ
Ordered Locus Names:BSU11010
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

S-methyl-L-methionine + L-homocysteine = 2 L-methionine.

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Zinc Potential.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Induction

Induced by methionine starvation. Ref.3

Sequence similarities

In the C-terminal section; belongs to the methylenetetrahydrofolate reductase family.

Contains 1 Hcy-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 612612Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
PRO_0000379125

Regions

Domain1 – 280280Hcy-binding
Region1 – 280280Homocysteine S-methyltransferase
Region295 – 6123185,10-methylenetetrahydrofolate reductase

Sites

Metal binding2001Zinc Potential
Metal binding2651Zinc Potential
Metal binding2661Zinc Potential

Sequences

Sequence LengthMass (Da)Tools
O06745 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: A7B6FFC2E648F800

FASTA61267,928
        10         20         30         40         50         60 
MGLLEDLQRQ VLIGDGAMGT LLYSYGIDRC FEELNISKPE EIQRIHKAYV EAGANIIQTN 

        70         80         90        100        110        120 
TYGANYIKLS RHGLEDDIKK MNQEAVKIAR ASAGDAYVLG TMGGIRTFNK NAYSLDEIKR 

       130        140        150        160        170        180 
SFREQLYLLL HEEPDGLLLE TYYDLEEARE VLKIARKETD LPIMLNVSMH EQGVLQDGTP 

       190        200        210        220        230        240 
LSDALRSIAD LGADIVGINC RLGPYHMIEA LSEVPIFDDV FLSVYPNSSL PSLEEGRLVY 

       250        260        270        280        290        300 
ETDDTYFQNS ASEFRKQGAR IIGGCCGTTP NHIRAMAEAV GGLAPITEKE VKTRAKEFIS 

       310        320        330        340        350        360 
VHHERTEPGL DEIAAKKRSI IVELDPPKKL SFDKFLSAAA ELKEAGIDAL TLADNSLATP 

       370        380        390        400        410        420 
RISNVACGAL VKQQLDMRSL VHITCRDRNI IGLQSHLMGL DTLGLNDVLA ITGDPSKIGD 

       430        440        450        460        470        480 
FPGATSVYDL TSFDLIRLIK QFNEGLSLSG KPLGKKTNFS VAAAFNPNVR HLDKAVKRLE 

       490        500        510        520        530        540 
KKIDCGADYF VSQPVYSEQQ LVDIHNETKH LKTPIYIGIM PLTSSRNAEF IHNEIPGIKL 

       550        560        570        580        590        600 
SDTIREKMAH AGEDKEKQKA EGLAIARSLL DTACELFNGI YLITPFLRSD LTAELTSYIQ 

       610 
QKDEQRQNIF LH 

« Hide

References

« Hide 'large scale' references
[1]"A Bacillus subtilis chromosome segment at the 100 degrees to 102 degrees position encoding 11 membrane proteins."
Roche B., Autret S., Levine A., Vannier F., Medina N., Seror S.J.
Microbiology 143:3309-3312(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in Gram-positive bacteria."
Grundy F.J., Henkin T.M.
Mol. Microbiol. 30:737-749(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: 168 / BR151.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09476 Genomic DNA. Translation: CAA70665.1.
AL009126 Genomic DNA. Translation: CAB12941.1.
PIRC69840.
RefSeqNP_388982.1. NC_000964.3.

3D structure databases

ProteinModelPortalO06745.
SMRO06745. Positions 2-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU11010.

Proteomic databases

PaxDbO06745.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12941; CAB12941; BSU11010.
GeneID939796.
KEGGbsu:BSU11010.
PATRIC18973908. VBIBacSub10457_1148.

Organism-specific databases

GenoListBSU11010.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000028409.
KOK00547.
OMAQSHLMGL.
OrthoDBEOG6SNDP1.
PhylomeDBO06745.

Enzyme and pathway databases

BioCycBSUB:BSU11010-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.20.20.220. 1 hit.
3.20.20.330. 1 hit.
InterProIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR003726. S_MeTrfase.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view]
SUPFAMSSF51730. SSF51730. 1 hit.
SSF82282. SSF82282. 1 hit.
PROSITEPS50970. HCY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYITJ_BACSU
AccessionPrimary (citable) accession number: O06745
Secondary accession number(s): Q796P9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList