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O06745

- YITJ_BACSU

UniProt

O06745 - YITJ_BACSU

Protein

Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase

Gene

yitJ

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    S-methyl-L-methionine + L-homocysteine = 2 L-methionine.
    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity
    Zinc.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi200 – 2001ZincPROSITE-ProRule annotation
    Metal bindingi265 – 2651ZincPROSITE-ProRule annotation
    Metal bindingi266 – 2661ZincPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC
    3. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, Metal-binding, NAD, NADP, S-adenosyl-L-methionine, Zinc

    Enzyme and pathway databases

    BioCyciBSUB:BSU11010-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
    Including the following 2 domains:
    Homocysteine S-methyltransferase (EC:2.1.1.10)
    Alternative name(s):
    S-methylmethionine:homocysteine methyltransferase
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:yitJ
    Ordered Locus Names:BSU11010
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU11010.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductasePRO_0000379125Add
    BLAST

    Proteomic databases

    PaxDbiO06745.

    Expressioni

    Inductioni

    Induced by methionine starvation.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU11010.

    Structurei

    3D structure databases

    ProteinModelPortaliO06745.
    SMRiO06745. Positions 2-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 280280Hcy-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 280280Homocysteine S-methyltransferaseAdd
    BLAST
    Regioni295 – 6123185,10-methylenetetrahydrofolate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the methylenetetrahydrofolate reductase family.Curated
    Contains 1 Hcy-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000028409.
    KOiK00547.
    OMAiQSHLMGL.
    OrthoDBiEOG6SNDP1.
    PhylomeDBiO06745.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    3.20.20.330. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR003726. S_MeTrfase.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    SSF82282. SSF82282. 1 hit.
    PROSITEiPS50970. HCY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O06745-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLLEDLQRQ VLIGDGAMGT LLYSYGIDRC FEELNISKPE EIQRIHKAYV    50
    EAGANIIQTN TYGANYIKLS RHGLEDDIKK MNQEAVKIAR ASAGDAYVLG 100
    TMGGIRTFNK NAYSLDEIKR SFREQLYLLL HEEPDGLLLE TYYDLEEARE 150
    VLKIARKETD LPIMLNVSMH EQGVLQDGTP LSDALRSIAD LGADIVGINC 200
    RLGPYHMIEA LSEVPIFDDV FLSVYPNSSL PSLEEGRLVY ETDDTYFQNS 250
    ASEFRKQGAR IIGGCCGTTP NHIRAMAEAV GGLAPITEKE VKTRAKEFIS 300
    VHHERTEPGL DEIAAKKRSI IVELDPPKKL SFDKFLSAAA ELKEAGIDAL 350
    TLADNSLATP RISNVACGAL VKQQLDMRSL VHITCRDRNI IGLQSHLMGL 400
    DTLGLNDVLA ITGDPSKIGD FPGATSVYDL TSFDLIRLIK QFNEGLSLSG 450
    KPLGKKTNFS VAAAFNPNVR HLDKAVKRLE KKIDCGADYF VSQPVYSEQQ 500
    LVDIHNETKH LKTPIYIGIM PLTSSRNAEF IHNEIPGIKL SDTIREKMAH 550
    AGEDKEKQKA EGLAIARSLL DTACELFNGI YLITPFLRSD LTAELTSYIQ 600
    QKDEQRQNIF LH 612
    Length:612
    Mass (Da):67,928
    Last modified:July 1, 1997 - v1
    Checksum:iA7B6FFC2E648F800
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09476 Genomic DNA. Translation: CAA70665.1.
    AL009126 Genomic DNA. Translation: CAB12941.1.
    PIRiC69840.
    RefSeqiNP_388982.1. NC_000964.3.
    WP_003245847.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12941; CAB12941; BSU11010.
    GeneIDi939796.
    KEGGibsu:BSU11010.
    PATRICi18973908. VBIBacSub10457_1148.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09476 Genomic DNA. Translation: CAA70665.1 .
    AL009126 Genomic DNA. Translation: CAB12941.1 .
    PIRi C69840.
    RefSeqi NP_388982.1. NC_000964.3.
    WP_003245847.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali O06745.
    SMRi O06745. Positions 2-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU11010.

    Proteomic databases

    PaxDbi O06745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12941 ; CAB12941 ; BSU11010 .
    GeneIDi 939796.
    KEGGi bsu:BSU11010.
    PATRICi 18973908. VBIBacSub10457_1148.

    Organism-specific databases

    GenoListi BSU11010.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000028409.
    KOi K00547.
    OMAi QSHLMGL.
    OrthoDBi EOG6SNDP1.
    PhylomeDBi O06745.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci BSUB:BSU11010-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    3.20.20.330. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR003726. S_MeTrfase.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    SSF82282. SSF82282. 1 hit.
    PROSITEi PS50970. HCY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Bacillus subtilis chromosome segment at the 100 degrees to 102 degrees position encoding 11 membrane proteins."
      Roche B., Autret S., Levine A., Vannier F., Medina N., Seror S.J.
      Microbiology 143:3309-3312(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in Gram-positive bacteria."
      Grundy F.J., Henkin T.M.
      Mol. Microbiol. 30:737-749(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: 168 / BR151.

    Entry informationi

    Entry nameiYITJ_BACSU
    AccessioniPrimary (citable) accession number: O06745
    Secondary accession number(s): Q796P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3