O06741 (YITF_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative isomerase yitF
EC=5.-.-.-

Gene names

Name:	yitF
Ordered Locus Names:	BSU10970

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Divalent metal cations. Magnesium Probable.
Subunit structure	Homooctamer. Ref.3
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 371

371

Putative isomerase yitF

PRO_0000360691

Sites

Active site

158

1

By similarity

Active site

290

1

By similarity

Metal binding

214

1

Magnesium

Metal binding

240

1

Magnesium

Metal binding

290

1

Magnesium

Secondary structure

1

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371

Helix Strand Turn

Details...

Beta strand

2 – 19

18

Beta strand

26 – 38

13

Helix

52 – 56

5

Turn

60 – 62

3

Helix

63 – 67

5

Helix

75 – 85

11

Helix

87 – 104

18

Helix

109 – 112

4

Beta strand

119 – 121

3

Beta strand

123 – 126

4

Helix

136 – 148

13

Beta strand

154 – 158

5

Helix

164 – 177

14

Beta strand

182 – 187

6

Helix

194 – 198

5

Helix

201 – 204

4

Helix

222 – 229

8

Helix

248 – 253

6

Beta strand

258 – 260

3

Turn

264 – 266

3

Helix

271 – 282

12

Helix

296 – 306

11

Beta strand

311 – 313

3

Beta strand

322 – 325

4

Helix

330 – 334

5

Helix

358 – 363

6

Sequences

	Sequence		Length	Mass (Da)
	O06741 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: C41FD69E471FE87E	FASTA	371	42,052
	10 20 30 40 50 60 MKIVRIETFP LFHRLEKPYG DANGFKRYRT CYLIRIITES GIDGWGECVD WLPALHVGFT 70 80 90 100 110 120 KRIIPFLLGK QAGSRLSLVR TIQKWHQRAA SAVSMALTEI AAKAADCSVC ELWGGRYREE 130 140 150 160 170 180 IPVYASFQSY SDSPQWISRS VSNVEAQLKK GFEQIKVKIG GTSFKEDVRH INALQHTAGS 190 200 210 220 230 240 SITMILDANQ SYDAAAAFKW ERYFSEWTNI GWLEEPLPFD QPQDYAMLRS RLSVPVAGGE 250 260 270 280 290 300 NMKGPAQYVP LLSQRCLDII QPDVMHVNGI DEFRDCLQLA RYFGVRASAH AYDGSLSRLY 310 320 330 340 350 360 ALFAQACLPP WSKMKNDHIE PIEWDVMENP FTDLVSLQPS KGMVHIPKGK GIGTEINMEI 370 VNRYKWDGSA Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Bacillus subtilis chromosome segment at the 100 degrees to 102 degrees position encoding 11 membrane proteins." Roche B., Autret S., Levine A., Vannier F., Medina N., Seror S.J. Microbiology 143:3309-3312(1997) [PubMed: 9353932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Crystal structure of mandelate racemase/muconate lactonizing enzyme from Bacillus subtilis at 1.8 A resolution." New York structural genomix research consortium (NYSGXRC) Submitted (APR-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09476 Genomic DNA. Translation: CAA70661.1.
AL009126 Genomic DNA. Translation: CAB12937.1.

PIR

G69839.

RefSeq

NP_388978.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GDQ	X-ray	1.80	A/B	2-371	[»]
2GGE	X-ray	1.89	A/B/C/D/E/F/G/H	2-371	[»]

ProteinModelPortal

O06741.

SMR

O06741. Positions 1-371.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000001515; EBBACP00000001515; EBBACG00000001513.

GeneID

939345.

GenomeReviews

Gene locus BSU10970 in contig AL009126_GR.

KEGG

bsu:BSU10970.

NMPDR

fig|224308.1.peg.1097.

PATRIC

18973900. VBIBacSub10457_1144.

Organism-specific databases

GenoList

BSU10970.

Phylogenomic databases

GeneTree

EBGT00070000032382.

HOGENOM

HBG348922.

OMA

EWDVMEN.

PhylomeDB

O06741.

ProtClustDB

CLSK237674.

Enzyme and pathway databases

BioCyc

BSUB:BSU10970-MONOMER.

Family and domain databases

InterPro

IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]

PANTHER

PTHR13794. MR_MLE. 1 hit.

Pfam

PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]

SMART

SM00922. MR_MLE. 1 hit.
[Graphical view]

PROSITE

PS00908. MR_MLE_1. False negative.
PS00909. MR_MLE_2. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YITF_BACSU

Accession

Primary (citable) accession number: O06741
Secondary accession number(s): Q796Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program