O06559 (NARG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase alpha subunit
EC=1.7.99.4

Gene names

Name:	narG
Ordered Locus Names:	Rv1161, MT1198

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1232 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The alpha chain is the actual site of nitrate reduction Probable. Supports anaerobic growth of E.coli on glycerol in an E.coli mutant lacking endogenous nitrate reductase. Ref.3
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Enzyme regulation	Increased nitrate reductase activity is seen under hypoxic conditions, however this seems to be due to induction of the probable nitrate/nitrite transporter narK2 rather than increased enzyme activity.
Subcellular location	Cell membrane; Peripheral membrane protein.
Induction	Constitutively expressed; independent of nitrate and nitrate levels. Ref.3
Disruption phenotype	Loss of nitrate reductase activity in aerobic and hypoxic conditions. Ref.3
Miscellaneous	One of the activities induced in M.tuberculosis by hypoxia is the dissimilatory reduction of nitrate to nitrite, which serves to provide energy as the bacteria adapt to anaerobiosis.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Cell membrane Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from mutant phenotype PubMed 10712684PubMed 19332834. Source: MTBBASE nitrate catabolic process Inferred from mutant phenotype Ref.3. Source: MTBBASE response to acid Inferred from direct assay PubMed 21048946. Source: MTBBASE
Cellular_component	cell wall Inferred from direct assay PubMed 20825248. Source: MTBBASE extracellular region Inferred from direct assay PubMed 17443846. Source: MTBBASE nitrate reductase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 15525680. Source: MTBBASE
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1232	1232	Nitrate reductase alpha subunit		PRO_0000393357
Sites
	Metal binding	60	1	Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
	Metal binding	64	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	68	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	103	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	233	1	Molybdenum By similarity

Sequences

	Sequence		Length	Mass (Da)
	O06559 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F12194DA383644F9	FASTA	1,232	136,956
	10 20 30 40 50 60 MTVTPHVGGP LEELLERSGR FFTPGEFSAD LRTVTRRGGR EGDVFYRDRW SHDKVVRSTH 70 80 90 100 110 120 GVNCTGSCSW KIYVKDGIIT WETQQTDYPS VGPDRPEYEP RGCPRGASFS WYSYSPTRVR 130 140 150 160 170 180 YPYARGVLVE MYREAKTRLG DPVLAWADIQ ADPERRRRYQ QARGKGGLVR VSWAEASEMV 190 200 210 220 230 240 AAAHVHTIKT YGPDRVAGFS PIPAMSMVSH AAGSRFVELI GGVMTSFYDW YADLPVASPQ 250 260 270 280 290 300 VFGDQTDVPE SGDWWDASYL VMWGSNVPIT RTPDAHWMAE ARYRGAKVVV VSPDYADNTK 310 320 330 340 350 360 FADEWVRCAA GTDTALAMAM GHVILSECYV RNQVPFFVDY VRRYTDLPFL IKLEKRGDLL 370 380 390 400 410 420 VPGKFLTAAD IGEESENAAF KPALLDELTN TVVVPQGSLG FRFGEDGVGK WNLDLGSVVP 430 440 450 460 470 480 ALSVEMDKAV NGDRSAELVT LPSFDTIDGH GETVSRGVPV RRAGKHLVCT VFDLMLAHYG 490 500 510 520 530 540 VARAGLPGEW PTGYHDRTQQ NTPAWQESIT GVPAAQAIRF AKEFARNATE SGGRSMIIMG 550 560 570 580 590 600 GGICHWFHSD VMYRSVLALL MLTGSMGRNG GGWAHYVGQE KVRPLTGWQT MAMATDWSRP 610 620 630 640 650 660 PRQVPGASYW YAHTDQWRYD GYGADKLASP VGRGRFAGKH TMDLLTSATA MGWSPFYPQF 670 680 690 700 710 720 DRSSLDVADE ARAAGRDVGD YVAEQLAQHK LKLSITDPDN PVNWPRVLTV WRANLIGSSG 730 740 750 760 770 780 KGGEYFLRHL LGTDSNVQSD PPTDGVHPRD VVWDSDIPEG KLDLIMSIDF RMTSTTLVSD 790 800 810 820 830 840 VVLPAATWYE KSDLSSTDMH PYVHSFSPAI DPPWETRSDF DAFAAIARAF SALAKRHLGT 850 860 870 880 890 900 RTDVVLTALQ HDTPDEMAYP DGTERDWLAT GEVPVPGRTM SKLTVVERDY TAIYDKWLTL 910 920 930 940 950 960 GPLIDQFGMT TKGYTVHPFR EVSELAANFG VMNSGVAVGR PAITTAKRMA DVILALSGTC 970 980 990 1000 1010 1020 NGRLAVEGFL ELEKRTGQRL AHLAEGSEER RITYADTQAR PVPVITSPEW SGSESGGRRY 1030 1040 1050 1060 1070 1080 APFTINIEHL KPFHTLTGRM HFYLAHDWVE ELGEQLPVYR PPLDMARLFN QPELGPTDDG 1090 1100 1110 1120 1130 1140 LGLTVRYLTP HSKWSFHSTY QDNLYMLSLS RGGPTMWMSP GDAAKINVRD NDWVEAVNAN 1150 1160 1170 1180 1190 1200 GIYVCRAIVS HRMPEGVVFV YHVQERTVDT PRTETNGKRG GNHNALTRVR IKPSHLAGGY 1210 1220 1230 GQHAFAFNYL GPTGNQRDEV TVVRRRSQEV RY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Role of narK2X and narGHJI in hypoxic upregulation of nitrate reduction by Mycobacterium tuberculosis." Sohaskey C.D., Wayne L.G. J. Bacteriol. 185:7247-7256(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, FUNCTION, DISRUPTION PHENOTYPE. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000516 Genomic DNA. Translation: AAK45455.1. BX842575 Genomic DNA. Translation: CAB09020.1. AL123456 Genomic DNA. Translation: CCP43917.1.
PIR	B70556.
RefSeq	NP_215677.1. NC_000962.3. NP_335641.1. NC_002755.2. YP_006514536.1. NC_018143.1.
3D structure databases
HSSP	HSSP built from PDB template 1Q16 based on UniProtKB P09152.
ProteinModelPortal	O06559.
SMR	O06559. Positions 56-327, 761-827, 1022-1207.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv1161.
Proteomic databases
PRIDE	O06559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK45455; AAK45455; MT1198.
GeneID	13319740. 885573. 924879.
KEGG	mtc:MT1198. mtu:Rv1161. mtv:RVBD_1161.
PATRIC	18124406. VBIMycTub22151_1322.
Organism-specific databases
TubercuList	Rv1161.
Phylogenomic databases
eggNOG	COG5013.
HOGENOM	HOG000237341.
KO	K00370.
OMA	RSQEVVY.
ProtClustDB	CLSK868288.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006468. NarG. [Graphical view]
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01580. narG. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARG_MYCTU

Accession

Primary (citable) accession number: O06559
Secondary accession number(s): L0T628, Q7D8Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program