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Protein

Cytolethal distending toxin subunit A

Gene

cdtA

Organism
Haemophilus ducreyi (strain 35000HP / ATCC 700724)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Cytolethal distending toxin subunit A
Short name:
CDT A
Gene namesi
Name:cdtA
Ordered Locus Names:HD_0902
OrganismiHaemophilus ducreyi (strain 35000HP / ATCC 700724)
Taxonomic identifieri233412 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000001022 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi91W → G: Abolishes toxicity towards intact cells; when associated with G-98; G-100 and G-102. 1 Publication1
Mutagenesisi98W → G: Abolishes toxicity towards intact cells; when associated with G-91; G-100 and G-102. 1 Publication1
Mutagenesisi100W → G: Abolishes toxicity towards intact cells; when associated with G-91; G-98 and G-102. 1 Publication1
Mutagenesisi102Y → G: Abolishes toxicity towards intact cells; when associated with G-91; G-98 and G-100. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15PROSITE-ProRule annotationAdd BLAST15
ChainiPRO_000001337116 – 223Cytolethal distending toxin subunit AAdd BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi16N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi16S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Subunit structurei

Heterotrimer of 3 subunits, CdtA, CdtB and CdtC.1 Publication

Protein-protein interaction databases

STRINGi233412.HD0902.

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 61Combined sources3
Helixi73 – 76Combined sources4
Beta strandi78 – 82Combined sources5
Beta strandi85 – 90Combined sources6
Beta strandi98 – 103Combined sources6
Helixi104 – 106Combined sources3
Helixi108 – 114Combined sources7
Beta strandi116 – 120Combined sources5
Beta strandi127 – 131Combined sources5
Turni132 – 134Combined sources3
Beta strandi137 – 141Combined sources5
Beta strandi144 – 148Combined sources5
Helixi155 – 157Combined sources3
Beta strandi159 – 164Combined sources6
Beta strandi169 – 173Combined sources5
Turni174 – 176Combined sources3
Beta strandi179 – 182Combined sources4
Beta strandi187 – 189Combined sources3
Beta strandi193 – 196Combined sources4
Beta strandi210 – 213Combined sources4
Beta strandi219 – 221Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SR4X-ray2.00A18-223[»]
ProteinModelPortaliO06522.
SMRiO06522.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO06522.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 212Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 102Mediates binding to target cellsCuratedAdd BLAST12

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106BMH. Bacteria.
ENOG410Y0HS. LUCA.
KOiK11013.
OMAiHREYFRF.
OrthoDBiPOG091H0LIO.

Family and domain databases

InterProiIPR015957. CDtoxinA.
IPR003558. CDtoxinA/C.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF03498. CDtoxinA. 1 hit.
[Graphical view]
PIRSFiPIRSF036516. CDT_A. 1 hit.
PRINTSiPR01387. CDTOXINA.
SUPFAMiSSF50370. SSF50370. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O06522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFLPSLLL MGSVACSSNQ RMNDYSQPES QSDLAPKSST IQPQPQPLLS
60 70 80 90 100
KTPSMSLNLL SSSGPNRQVL PSEPSNFMTL MGQNGALLTV WALAKRNWLW
110 120 130 140 150
AYPNIYSQDF GNIRNWKMEP GKHREYFRFV NQSLGTCVEA YGNGLIHDIC
160 170 180 190 200
SLDKLAQEFE LLPTDSGAVV IKSVSQGRCV TYNPVSTTFY STVTLSVCDG
210 220
ATEPSRDQTW YLAPPVLEAT AVN
Length:223
Mass (Da):24,664
Last modified:July 1, 1997 - v1
Checksum:i5CA68F05958CD56D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53215 Genomic DNA. Translation: AAB57725.1.
AE017143 Genomic DNA. Translation: AAP95786.1.
RefSeqiWP_010944836.1. NC_002940.2.

Genome annotation databases

EnsemblBacteriaiAAP95786; AAP95786; HD_0902.
GeneIDi24944846.
KEGGihdu:HD_0902.
PATRICi20178201. VBIHaeDuc133973_0743.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53215 Genomic DNA. Translation: AAB57725.1.
AE017143 Genomic DNA. Translation: AAP95786.1.
RefSeqiWP_010944836.1. NC_002940.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SR4X-ray2.00A18-223[»]
ProteinModelPortaliO06522.
SMRiO06522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233412.HD0902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP95786; AAP95786; HD_0902.
GeneIDi24944846.
KEGGihdu:HD_0902.
PATRICi20178201. VBIHaeDuc133973_0743.

Phylogenomic databases

eggNOGiENOG4106BMH. Bacteria.
ENOG410Y0HS. LUCA.
KOiK11013.
OMAiHREYFRF.
OrthoDBiPOG091H0LIO.

Miscellaneous databases

EvolutionaryTraceiO06522.

Family and domain databases

InterProiIPR015957. CDtoxinA.
IPR003558. CDtoxinA/C.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF03498. CDtoxinA. 1 hit.
[Graphical view]
PIRSFiPIRSF036516. CDT_A. 1 hit.
PRINTSiPR01387. CDTOXINA.
SUPFAMiSSF50370. SSF50370. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDTA_HAEDU
AccessioniPrimary (citable) accession number: O06522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.