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O06493 (OPUE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Osmoregulated proline transporter
Alternative name(s):
Sodium/proline symporter
Gene names
Name:opuE
Synonyms:yerK
Ordered Locus Names:BSU06660
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the sodium-dependent uptake of extracellular proline.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

Ontologies

Keywords
   Biological processIon transport
Sodium transport
Symport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionproline:sodium symporter activity

Inferred from electronic annotation. Source: InterPro

sodium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Osmoregulated proline transporter
PRO_0000105404

Regions

Transmembrane3 – 2321Helical; Potential
Transmembrane62 – 8221Helical; Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane161 – 18121Helical; Potential
Transmembrane191 – 21121Helical; Potential
Transmembrane224 – 24421Helical; Potential
Transmembrane271 – 29121Helical; Potential
Transmembrane314 – 33421Helical; Potential
Transmembrane365 – 38521Helical; Potential
Transmembrane394 – 41421Helical; Potential
Transmembrane424 – 44421Helical; Potential
Transmembrane449 – 46921Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
O06493 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 23459873F1E799E6

FASTA49253,283
        10         20         30         40         50         60 
MSIEIIISLG IYFIAMLLIG WYAFKKTTDI NDYMLGGRGL GPFVTALSAG AADMSGWMLM 

        70         80         90        100        110        120 
GVPGAMFATG LSTLWLALGL TIGAYSNYLL LAPRLRAYTE AADDAITIPD FFDKRFQHSS 

       130        140        150        160        170        180 
SLLKIVSALI IMIFFTLYTS SGMVSGGRLF ESAFGADYKL GLFLTTAVVV LYTLFGGFLA 

       190        200        210        220        230        240 
VSLTDFVQGA IMFAALVLVP IVAFTHVGGV APTFHEIDAV NPHLLDIFKG ASVISIISYL 

       250        260        270        280        290        300 
AWGLGYYGQP HIIVRFMAIK DIKDLKPARR IGMSWMIITV LGSVLTGLIG VAYAHKFGVA 

       310        320        330        340        350        360 
VKDPEMIFII FSKILFHPLI TGFLLSAILA AIMSSISSQL LVTASAVTED LYRSFFRRKA 

       370        380        390        400        410        420 
SDKELVMIGR LSVLVIAVIA VLLSLNPNST ILDLVGYAWA GFGSAFGPAI LLSLYWKRMN 

       430        440        450        460        470        480 
EWGALAAMIV GAATVLIWIT TGLAKSTGVY EIIPGFILSM IAGIIVSMIT KRPAKASYRL 

       490 
FGVMEKLLKR KK

« Hide

References

« Hide 'large scale' references
[1]"Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B."
von Blohn C., Kempf B., Kappes R.M., Bremer E.
Mol. Microbiol. 25:175-187(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: a region devoted to purine uptake and metabolism, and containing the genes cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide sequence."
Borriss R., Porwollik S., Schroeter R.
Microbiology 142:3027-3031(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U92466 Genomic DNA. Translation: AAB66512.1.
AF011545 Genomic DNA. Translation: AAB72182.1.
AL009126 Genomic DNA. Translation: CAB12486.1.
PIRH69670.
RefSeqNP_388548.1. NC_000964.3.

3D structure databases

ProteinModelPortalO06493.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU06660.

Proteomic databases

PaxDbO06493.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12486; CAB12486; BSU06660.
GeneID939439.
KEGGbsu:BSU06660.
PATRIC18972978. VBIBacSub10457_0703.

Organism-specific databases

GenoListBSU06660. [Micado]

Phylogenomic databases

eggNOGCOG0591.
HOGENOMHOG000282935.
KOK03307.
OMAVAIWIAW.
ProtClustDBCLSK886821.

Enzyme and pathway databases

BioCycBSUB:BSU06660-MONOMER.

Family and domain databases

InterProIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERPTHR11819. PTHR11819. 1 hit.
PfamPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOPUE_BACSU
AccessionPrimary (citable) accession number: O06493
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

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