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Protein

Osmoregulated proline transporter OpuE

Gene

opuE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the uptake of extracellular proline under high-osmolarity growth conditions. Essential for the use of proline present in the environment as an osmoprotectant.2 Publications

Kineticsi

  1. KM=12 µM for proline1 Publication
  1. Vmax=27 nmol/min/mg enzyme1 Publication
  2. Vmax=19 nmol/min/mg enzyme (in the presence of 1 mM proline)1 Publication
  3. Vmax=104 nmol/min/mg enzyme (in the presence of 0.4 M NaCl)1 Publication
  4. Vmax=252 nmol/min/mg enzyme (in the presence of 0.6 M NaCl)1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Stress response, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU06660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Osmoregulated proline transporter OpuECurated
Alternative name(s):
Osmoprotectant uptake1 Publication
Proline uptake system1 Publication
Gene namesi
Name:opuE1 Publication
Synonyms:yerK
Ordered Locus Names:BSU06660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Transmembranei40 – 60HelicalSequence analysisAdd BLAST21
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Transmembranei271 – 291HelicalSequence analysisAdd BLAST21
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Transmembranei394 – 414HelicalSequence analysisAdd BLAST21
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Transmembranei449 – 469HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of the gene eliminates the osmotically stimulated proline transport activity and practically abolishes the ability to use proline as an osmoprotectant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054041 – 492Osmoregulated proline transporter OpuEAdd BLAST492

Proteomic databases

PaxDbiO06493.
PRIDEiO06493.

Expressioni

Inductioni

Induced under high-osmolarity growth conditions. Has two osmoregulated and tightly spaced promoters. The first promoter is sigma A-dependent and the second promoter is controlled by the general stress transcription factor sigma B.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003763.

Structurei

3D structure databases

ProteinModelPortaliO06493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK7. Bacteria.
COG0591. LUCA.
HOGENOMiHOG000282935.
InParanoidiO06493.
KOiK03307.
OMAiRTFFRRE.
PhylomeDBiO06493.

Family and domain databases

CDDicd11475. SLC5sbd_PutP. 1 hit.
InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PTHR11819:SF148. PTHR11819:SF148. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O06493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEIIISLG IYFIAMLLIG WYAFKKTTDI NDYMLGGRGL GPFVTALSAG
60 70 80 90 100
AADMSGWMLM GVPGAMFATG LSTLWLALGL TIGAYSNYLL LAPRLRAYTE
110 120 130 140 150
AADDAITIPD FFDKRFQHSS SLLKIVSALI IMIFFTLYTS SGMVSGGRLF
160 170 180 190 200
ESAFGADYKL GLFLTTAVVV LYTLFGGFLA VSLTDFVQGA IMFAALVLVP
210 220 230 240 250
IVAFTHVGGV APTFHEIDAV NPHLLDIFKG ASVISIISYL AWGLGYYGQP
260 270 280 290 300
HIIVRFMAIK DIKDLKPARR IGMSWMIITV LGSVLTGLIG VAYAHKFGVA
310 320 330 340 350
VKDPEMIFII FSKILFHPLI TGFLLSAILA AIMSSISSQL LVTASAVTED
360 370 380 390 400
LYRSFFRRKA SDKELVMIGR LSVLVIAVIA VLLSLNPNST ILDLVGYAWA
410 420 430 440 450
GFGSAFGPAI LLSLYWKRMN EWGALAAMIV GAATVLIWIT TGLAKSTGVY
460 470 480 490
EIIPGFILSM IAGIIVSMIT KRPAKASYRL FGVMEKLLKR KK
Length:492
Mass (Da):53,283
Last modified:July 1, 1997 - v1
Checksum:i23459873F1E799E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92466 Genomic DNA. Translation: AAB66512.1.
AF011545 Genomic DNA. Translation: AAB72182.1.
AL009126 Genomic DNA. Translation: CAB12486.1.
PIRiH69670.
RefSeqiNP_388548.1. NC_000964.3.
WP_003242814.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12486; CAB12486; BSU06660.
GeneIDi939439.
KEGGibsu:BSU06660.
PATRICi18972978. VBIBacSub10457_0703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92466 Genomic DNA. Translation: AAB66512.1.
AF011545 Genomic DNA. Translation: AAB72182.1.
AL009126 Genomic DNA. Translation: CAB12486.1.
PIRiH69670.
RefSeqiNP_388548.1. NC_000964.3.
WP_003242814.1. NZ_JNCM01000032.1.

3D structure databases

ProteinModelPortaliO06493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003763.

Proteomic databases

PaxDbiO06493.
PRIDEiO06493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12486; CAB12486; BSU06660.
GeneIDi939439.
KEGGibsu:BSU06660.
PATRICi18972978. VBIBacSub10457_0703.

Phylogenomic databases

eggNOGiENOG4105CK7. Bacteria.
COG0591. LUCA.
HOGENOMiHOG000282935.
InParanoidiO06493.
KOiK03307.
OMAiRTFFRRE.
PhylomeDBiO06493.

Enzyme and pathway databases

BioCyciBSUB:BSU06660-MONOMER.

Family and domain databases

CDDicd11475. SLC5sbd_PutP. 1 hit.
InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PTHR11819:SF148. PTHR11819:SF148. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPUE_BACSU
AccessioniPrimary (citable) accession number: O06493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: October 5, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.