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Protein

Osmoregulated proline transporter

Gene

opuE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the sodium-dependent uptake of extracellular proline.

GO - Molecular functioni

  1. proline:sodium symporter activity Source: InterPro
  2. sodium ion binding Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciBSUB:BSU06660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Osmoregulated proline transporter
Alternative name(s):
Sodium/proline symporter
Gene namesi
Name:opuE
Synonyms:yerK
Ordered Locus Names:BSU06660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU06660. [Micado]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence AnalysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence AnalysisAdd
BLAST
Transmembranei191 – 21121HelicalSequence AnalysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence AnalysisAdd
BLAST
Transmembranei271 – 29121HelicalSequence AnalysisAdd
BLAST
Transmembranei314 – 33421HelicalSequence AnalysisAdd
BLAST
Transmembranei365 – 38521HelicalSequence AnalysisAdd
BLAST
Transmembranei394 – 41421HelicalSequence AnalysisAdd
BLAST
Transmembranei424 – 44421HelicalSequence AnalysisAdd
BLAST
Transmembranei449 – 46921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Osmoregulated proline transporterPRO_0000105404Add
BLAST

Proteomic databases

PaxDbiO06493.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU06660.

Structurei

3D structure databases

ProteinModelPortaliO06493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0591.
HOGENOMiHOG000282935.
InParanoidiO06493.
KOiK03307.
OMAiNPEETRG.
OrthoDBiEOG6HB9QC.
PhylomeDBiO06493.

Family and domain databases

InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O06493-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIEIIISLG IYFIAMLLIG WYAFKKTTDI NDYMLGGRGL GPFVTALSAG
60 70 80 90 100
AADMSGWMLM GVPGAMFATG LSTLWLALGL TIGAYSNYLL LAPRLRAYTE
110 120 130 140 150
AADDAITIPD FFDKRFQHSS SLLKIVSALI IMIFFTLYTS SGMVSGGRLF
160 170 180 190 200
ESAFGADYKL GLFLTTAVVV LYTLFGGFLA VSLTDFVQGA IMFAALVLVP
210 220 230 240 250
IVAFTHVGGV APTFHEIDAV NPHLLDIFKG ASVISIISYL AWGLGYYGQP
260 270 280 290 300
HIIVRFMAIK DIKDLKPARR IGMSWMIITV LGSVLTGLIG VAYAHKFGVA
310 320 330 340 350
VKDPEMIFII FSKILFHPLI TGFLLSAILA AIMSSISSQL LVTASAVTED
360 370 380 390 400
LYRSFFRRKA SDKELVMIGR LSVLVIAVIA VLLSLNPNST ILDLVGYAWA
410 420 430 440 450
GFGSAFGPAI LLSLYWKRMN EWGALAAMIV GAATVLIWIT TGLAKSTGVY
460 470 480 490
EIIPGFILSM IAGIIVSMIT KRPAKASYRL FGVMEKLLKR KK
Length:492
Mass (Da):53,283
Last modified:July 1, 1997 - v1
Checksum:i23459873F1E799E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92466 Genomic DNA. Translation: AAB66512.1.
AF011545 Genomic DNA. Translation: AAB72182.1.
AL009126 Genomic DNA. Translation: CAB12486.1.
PIRiH69670.
RefSeqiNP_388548.1. NC_000964.3.
WP_003242814.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12486; CAB12486; BSU06660.
GeneIDi939439.
KEGGibsu:BSU06660.
PATRICi18972978. VBIBacSub10457_0703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92466 Genomic DNA. Translation: AAB66512.1.
AF011545 Genomic DNA. Translation: AAB72182.1.
AL009126 Genomic DNA. Translation: CAB12486.1.
PIRiH69670.
RefSeqiNP_388548.1. NC_000964.3.
WP_003242814.1. NZ_JNCM01000032.1.

3D structure databases

ProteinModelPortaliO06493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU06660.

Proteomic databases

PaxDbiO06493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12486; CAB12486; BSU06660.
GeneIDi939439.
KEGGibsu:BSU06660.
PATRICi18972978. VBIBacSub10457_0703.

Organism-specific databases

GenoListiBSU06660. [Micado]

Phylogenomic databases

eggNOGiCOG0591.
HOGENOMiHOG000282935.
InParanoidiO06493.
KOiK03307.
OMAiNPEETRG.
OrthoDBiEOG6HB9QC.
PhylomeDBiO06493.

Enzyme and pathway databases

BioCyciBSUB:BSU06660-MONOMER.

Family and domain databases

InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B."
    von Blohn C., Kempf B., Kappes R.M., Bremer E.
    Mol. Microbiol. 25:175-187(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  2. "The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: a region devoted to purine uptake and metabolism, and containing the genes cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide sequence."
    Borriss R., Porwollik S., Schroeter R.
    Microbiology 142:3027-3031(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiOPUE_BACSU
AccessioniPrimary (citable) accession number: O06493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.