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Protein

Benzaldehyde dehydrogenase YfmT

Gene

yfmT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A benzaldehyde dehydrogenase able to act on substrates with 3- and 4-hydroxy and methoxy substitutions; converts vanillin (4-hydroxy-3-methoxybenzaldehyde) to vanillic acid in vitro (PubMed:26658822). The physiological substrate is unknown (PubMed:26658822).1 Publication

Catalytic activityi

Benzaldehyde + NAD+ + H2O = benzoate + NADH.1 Publication
Vanillin + NAD+ + H2O = vanillate + NADH.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 37-40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei253PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi231 – 236NADBy similarity6

GO - Molecular functioni

  • benzaldehyde dehydrogenase (NAD+) activity Source: UniProtKB
  • vanillin dehydrogenase activity Source: UniProtKB-EC

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciBSUB:BSU07350-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Benzaldehyde dehydrogenase YfmTPROSITE-ProRule annotation1 Publication (EC:1.2.1.281 Publication)
Alternative name(s):
Vanillin dehydrogenase1 Publication (EC:1.2.1.671 Publication)
Gene namesi
Name:yfmT
Ordered Locus Names:BSU07350
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Loss of conversion of vanillin to vanillic acid (PubMed:26658822).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003795101 – 485Benzaldehyde dehydrogenase YfmTAdd BLAST485

Proteomic databases

PaxDbiO06478
PRIDEiO06478

Expressioni

Inductioni

Transcriptionally regulated by SigD; part of the yfmT/yfmS operon.1 Publication

Interactioni

Protein-protein interaction databases

IntActiO06478, 1 interactor
MINTiO06478
STRINGi224308.Bsubs1_010100004118

Structurei

3D structure databases

ProteinModelPortaliO06478
SMRiO06478
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG1012 LUCA
HOGENOMiHOG000271509
InParanoidiO06478
KOiK21802
OMAiTYVLKHA
PhylomeDBiO06478

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

O06478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQYEELNKQ FIGGKWQEGS SPNVLENKNP YTQKTFTTFR KATADDVDEA
60 70 80 90 100
YRAAALAKKK WDAVNPFEKR TILEKAVTYI EENEEAIIYL IMEELGGTRL
110 120 130 140 150
KAAFEIGLVK NIIKEAATFP IRMEGKILPS TIDGKENRLY RVPAGVVGVI
160 170 180 190 200
SPFNFPFFLS MKSVAPALGA GNGVVLKPHE ETPICGGTLI AKIFENAGIP
210 220 230 240 250
AGLLNVVVTD IAEIGDSFVE HPVPRIISFT GSTKVGSYIG QLAMKHFKKP
260 270 280 290 300
LLELGGNSAF IVLEDADIEY AVNAAVFSRF THQGQICMSA NRVLVHSSIY
310 320 330 340 350
DKFLELYQAK VESLKVGDPM DPDTIIGPLI NSRQTDGLMK TVEQAIEEGA
360 370 380 390 400
VPVKLGGFNG TIVEPTILKD VKPFMSIAKE ELFGPVVSFM KFDSEDEAVD
410 420 430 440 450
IANETPFGLS GAVHTSNLER GVAFAKRIET GMIHVNDTTI NDEPNVAFGG
460 470 480
EKQSGLGRLN GEWSLEEFTT LKWISVQHEK RSFPY
Length:485
Mass (Da):53,321
Last modified:July 1, 1997 - v1
Checksum:iC87E9AA12C704B68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86418 Genomic DNA Translation: BAA20109.1
AL009126 Genomic DNA Translation: CAB12554.1
PIRiC69814
RefSeqiNP_388616.1, NC_000964.3
WP_003244104.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12554; CAB12554; BSU07350
GeneIDi938788
KEGGibsu:BSU07350
PATRICifig|224308.179.peg.797

Similar proteinsi

Entry informationi

Entry nameiALDH5_BACSU
AccessioniPrimary (citable) accession number: O06478
Secondary accession number(s): Q797A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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