Reviewed, UniProtKB/Swiss-Prot O06465 (AHPF_XANCH)

Names and origin

Protein names

Recommended name:
    Alkyl hydroperoxide reductase subunit F
    EC=1.6.4.-

Gene names

Name:

ahpF

Organism

Xanthomonas campestris pv. phaseoli

Taxonomic identifier

29445 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the ahpC protein.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on NADH or NADPH Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Alkyl hydroperoxide reductase subunit F		PRO_0000166787
Regions
	Nucleotide binding	214 – 229	16	FAD By similarity
	Nucleotide binding	356 – 370	15	NAD or NADP By similarity
	Nucleotide binding	477 – 487	11	FAD By similarity
Amino acid modifications
	Disulfide bond	344 ↔ 347		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	O06465-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: ABC1105C8A6FCD24	FASTA	530	56,922
	10 20 30 40 50 60 MLDANLKTQL TAYLERVTRP IQINASIDDS AGSREMLDLL EELVLLSDKI SLDIHRDDNQ 70 80 90 100 110 120 RKPSFALTTP GQDISLRFAG LPMGHEFTSL VLALLQVGGH PSKAAAELIE QVQHLEGDYQ 130 140 150 160 170 180 FETYFSLSCQ NCPDVVQALN LAAVLNPRIK HVAIDGAWFQ DEVQARQIMS VPTVYLNGEL 190 200 210 220 230 240 FDQGRMTLEQ IVAKLDTNAA KRDAAKIAAK EAFDVLVVGG GPAGSAAAVY AARKGIRTGV 250 260 270 280 290 300 AAERFGGQVL DTMSIENFIS VPETEGPKMA AALEQHVRQY DVDIMNLQRA EQLIPAGADG 310 320 330 340 350 360 LIEIKLANGA SLKSKTVILS TGARWRQMNV PGEDQYKNKG VAYCPHCDGP LFKGKRVAVI 370 380 390 400 410 420 GGGNSGVEAA IDLAGIVAHV TLVEFDDKLR ADEVLQRKLR SLHNVRIITS AQTTEVLGDG 430 440 450 460 470 480 QKVTGLVYKD RTGGDIQHIE LEGVFVQIGL LPNTEFLRGT VALSPRGEII VDDRGQTDVP 490 500 510 520 530 GVFAAGDATT VPYKQIVIAM GEGSKAALSA FDHLIRTSAP ATADSVAQAA

References

[1]

"Isolation and analysis of the Xanthomonas alkyl hydroperoxide reductase gene and the peroxide sensor regulator genes ahpC and ahpF-oxyR-orfX." Loprasert S., Atichartpongkul S., Whangsuk W., Mongkolsuk S. J. Bacteriol. 179:3944-3949(1997) [PubMed: 9190810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U94336 Genomic DNA. Translation: AAC45426.1.
3D structure databases
HSSP	HSSP built from PDB template 1FL2 based on UniProtKB P35340.
ModBase	Search...
Proteomic databases
PRIDE	O06465.
Family and domain databases
InterPro	IPR012081. Alkyl_hydroperoxide_Rdtase_suF. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR002109. Glutaredoxin. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF000238. AhpF. 1 hit.
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03140. AhpF. 1 hit.
PROSITE	PS51354. GLUTAREDOXIN_2. 1 hit. PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AHPF_XANCH

Accession

Primary (citable) accession number: O06465

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	March 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)