Reviewed, UniProtKB/Swiss-Prot O06457 (PURK_SULSO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	SSO1065

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075020
Regions
	Domain	106 – 286	181	ATP-grasp
	Nucleotide binding	177 – 180	4	ATP By similarity
	Nucleotide binding	256 – 257	2	ATP By similarity
Sites
	Binding site	102	1	ATP By similarity
	Binding site	143	1	ATP By similarity
	Binding site	154	1	ATP By similarity
	Binding site	185	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O06457-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: A9EE75D029EE199C	FASTA	365	42,360
	10 20 30 40 50 60 MFSVLDWKPK IGILGGGQLG WMIVLEGRKY PFTFYVLEND KNAPACRIAD RCFSPQDYKE 70 80 90 100 110 120 FVDSSDVITF EFEHVYEKAL EYAEYSGKLL PRLNSVELKR ERYKEKLFYR QHNLPTPRFY 130 140 150 160 170 180 VAEDGEEALK ILREEFNNVG VIKESKGGYD GKGQYFIFND VEKYQFLREK KEKMVVEEYV 190 200 210 220 230 240 KFDFEASIII ARDKRGVFIS YPPTYNYNEK GILVYNYGPY NNQNIVEIAR RLSEELDYVG 250 260 270 280 290 300 IMGVEVFVVN GKVLINEFAP RVHNTGHYTL DGALISQFEQ HLRAIIGMEL GPSTILSPSG 310 320 330 340 350 360 MVNILGTDKI PVEVLKYGKV YWYSKSEVRK RRKMGHVNVV GNNLEEVKQK IDKIMQLIYT NGLDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes." Soerensen I.S., Dandanell G. FEMS Microbiol. Lett. 154:173-180(1997) [PubMed: 9311113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13143 Genomic DNA. Translation: CAA73603.1. AE006641 Genomic DNA. Translation: AAK41328.1.
PIR	A90259.
RefSeq	NP_342538.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Genome annotation databases
GeneID	1454107.
GenomeReviews	Gene locus SSO1065 in contig AE006641_GR.
KEGG	sso:SSO1065.
NMPDR	fig|273057.1.peg.961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O06457.
OMA	FDQQLCI.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1065-MON.
BRENDA	4.1.1.21. 2070.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_SULSO

Accession

Primary (citable) accession number: O06457

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)