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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Prefers MTA, with 2% activity on adenosine, 0.8% activity on S-adenosyl-L-homocysteine and no activity on other tested nucleosides.

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation1 Publication

Enzyme regulationi

Not inhibited by adenosine, potently inhibited by MT-DADMe-immucillin A.1 Publication

Kineticsi

  1. KM=9 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=260 µM for phosphate1 Publication
  3. KM=1700 µM for adenosine1 Publication

    pH dependencei

    Optimum pH is 7-7.5.1 Publication

    Pathway: L-methionine biosynthesis via salvage pathway

    This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
    This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141PhosphateUniRule annotation
    Sitei162 – 1621Important for substrate specificityUniRule annotation
    Binding sitei180 – 1801Substrate; via amide nitrogenUniRule annotation
    Binding sitei181 – 1811PhosphateUniRule annotation
    Sitei217 – 2171Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMTBRV:RV0535-MONOMER.
    BRENDAi2.4.2.28. 3445.
    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Gene namesi
    Name:mtnPUniRule annotation
    Ordered Locus Names:Rv0535
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv0535.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264S-methyl-5'-thioadenosine phosphorylasePRO_0000415096Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi83332.Rv0535.

    Structurei

    3D structure databases

    ProteinModelPortaliO06401.
    SMRiO06401. Positions 2-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni55 – 562Phosphate bindingUniRule annotation
    Regioni204 – 2063Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000228987.
    InParanoidiO06401.
    KOiK00772.
    OMAiMTNHTEA.
    OrthoDBiEOG6KHFXC.
    PhylomeDBiO06401.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O06401-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MHNNGRMLGV IGGSGFYTFF GSDTRTVNSD TPYGQPSAPI TIGTIGVHDV
    60 70 80 90 100
    AFLPRHGAHH QYSAHAVPYR ANMWALRALG VRRVFGPCAV GSLDPELEPG
    110 120 130 140 150
    AVVVPDQLVD RTSGRADTYF DFGGVHAAFA DPYCPTLRAA VTGLPGVVDG
    160 170 180 190 200
    GTMVVIQGPR FSTRAESQWF AAAGCNLVNM TGYPEAVLAR ELELCYAAIA
    210 220 230 240 250
    LVTDVDAGVA AGDGVKAADV FAAFGENIEL LKRLVRAAID RVADERTCTH
    260
    CQHHAGVPLP FELP
    Length:264
    Mass (Da):27,964
    Last modified:July 1, 1997 - v1
    Checksum:i3A850EA1B1873662
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43273.1.
    PIRiB70546.
    RefSeqiNP_215049.1. NC_000962.3.
    WP_003402867.1. NZ_KK339370.1.
    YP_006513868.1. NC_018143.2.

    Genome annotation databases

    EnsemblBacteriaiCCP43273; CCP43273; Rv0535.
    GeneIDi887430.
    KEGGimtu:Rv0535.
    mtv:RVBD_0535.
    PATRICi18149684. VBIMycTub87468_0594.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43273.1.
    PIRiB70546.
    RefSeqiNP_215049.1. NC_000962.3.
    WP_003402867.1. NZ_KK339370.1.
    YP_006513868.1. NC_018143.2.

    3D structure databases

    ProteinModelPortaliO06401.
    SMRiO06401. Positions 2-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv0535.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCCP43273; CCP43273; Rv0535.
    GeneIDi887430.
    KEGGimtu:Rv0535.
    mtv:RVBD_0535.
    PATRICi18149684. VBIMycTub87468_0594.

    Organism-specific databases

    TubercuListiRv0535.

    Phylogenomic databases

    HOGENOMiHOG000228987.
    InParanoidiO06401.
    KOiK00772.
    OMAiMTNHTEA.
    OrthoDBiEOG6KHFXC.
    PhylomeDBiO06401.

    Enzyme and pathway databases

    UniPathwayiUPA00904; UER00873.
    BioCyciMTBRV:RV0535-MONOMER.
    BRENDAi2.4.2.28. 3445.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.
    3. "Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis."
      Buckoreelall K., Sun Y., Hobrath J.V., Wilson L., Parker W.B.
      Tuberculosis 92:139-147(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT.
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiMTAP_MYCTU
    AccessioniPrimary (citable) accession number: O06401
    Secondary accession number(s): L0T6S7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: July 1, 1997
    Last modified: June 24, 2015
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.