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O06401

- MTAP_MYCTU

UniProt

O06401 - MTAP_MYCTU

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Protein
S-methyl-5'-thioadenosine phosphorylase
Gene
mtnP, Rv0535
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Prefers MTA, with 2% activity on adenosine, 0.8% activity on S-adenosyl-L-homocysteine and no activity on other tested nucleosides.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.1 Publication

Enzyme regulationi

Not inhibited by adenosine, potently inhibited by MT-DADMe-immucillin A.1 Publication

Kineticsi

  1. KM=9 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=260 µM for phosphate
  3. KM=1700 µM for adenosine

pH dependencei

Optimum pH is 7-7.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Phosphate By similarity
Sitei162 – 1621Important for substrate specificity By similarity
Binding sitei180 – 1801Substrate; via amide nitrogen By similarity
Binding sitei181 – 1811Phosphate By similarity
Sitei217 – 2171Important for substrate specificity By similarity

GO - Molecular functioni

  1. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP
  2. phosphorylase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. L-methionine salvage from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMTBRV:RV0535-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylase (EC:2.4.2.28)
Alternative name(s):
5'-methylthioadenosine phosphorylase
Short name:
MTA phosphorylase
Short name:
MTAP
Gene namesi
Name:mtnP
Ordered Locus Names:Rv0535
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv0535.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264S-methyl-5'-thioadenosine phosphorylaseUniRule annotation
PRO_0000415096Add
BLAST

Interactioni

Subunit structurei

Homodimer Inferred.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv0535.

Structurei

3D structure databases

ProteinModelPortaliO06401.
SMRiO06401. Positions 2-248.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate binding By similarity
Regioni204 – 2063Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000228987.
KOiK00772.
OMAiCEAQLCY.
OrthoDBiEOG6KHFXC.
PhylomeDBiO06401.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

O06401-1 [UniParc]FASTAAdd to Basket

« Hide

MHNNGRMLGV IGGSGFYTFF GSDTRTVNSD TPYGQPSAPI TIGTIGVHDV    50
AFLPRHGAHH QYSAHAVPYR ANMWALRALG VRRVFGPCAV GSLDPELEPG 100
AVVVPDQLVD RTSGRADTYF DFGGVHAAFA DPYCPTLRAA VTGLPGVVDG 150
GTMVVIQGPR FSTRAESQWF AAAGCNLVNM TGYPEAVLAR ELELCYAAIA 200
LVTDVDAGVA AGDGVKAADV FAAFGENIEL LKRLVRAAID RVADERTCTH 250
CQHHAGVPLP FELP 264
Length:264
Mass (Da):27,964
Last modified:July 1, 1997 - v1
Checksum:i3A850EA1B1873662
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43273.1.
PIRiB70546.
RefSeqiNP_215049.1. NC_000962.3.
YP_006513868.1. NC_018143.2.

Genome annotation databases

EnsemblBacteriaiCCP43273; CCP43273; Rv0535.
GeneIDi13318409.
887430.
KEGGimtu:Rv0535.
mtv:RVBD_0535.
PATRICi18149684. VBIMycTub87468_0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43273.1 .
PIRi B70546.
RefSeqi NP_215049.1. NC_000962.3.
YP_006513868.1. NC_018143.2.

3D structure databases

ProteinModelPortali O06401.
SMRi O06401. Positions 2-248.
ModBasei Search...

Protein-protein interaction databases

STRINGi 83332.Rv0535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCP43273 ; CCP43273 ; Rv0535 .
GeneIDi 13318409.
887430.
KEGGi mtu:Rv0535.
mtv:RVBD_0535.
PATRICi 18149684. VBIMycTub87468_0594.

Organism-specific databases

TubercuListi Rv0535.

Phylogenomic databases

HOGENOMi HOG000228987.
KOi K00772.
OMAi CEAQLCY.
OrthoDBi EOG6KHFXC.
PhylomeDBi O06401.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00873 .
BioCyci MTBRV:RV0535-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  3. "Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis."
    Buckoreelall K., Sun Y., Hobrath J.V., Wilson L., Parker W.B.
    Tuberculosis 92:139-147(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBUNIT.
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiMTAP_MYCTU
AccessioniPrimary (citable) accession number: O06401
Secondary accession number(s): L0T6S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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