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Protein

Carboxylesterase LipF

Gene

lipF

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes short-chain esters. Shows maximal activity with triacetin and p-nitrophenyl acetate.1 Publication

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.1 Publication

Kineticsi

kcat is 581.2 sec(-1) for triacetin. kcat is 223.7 sec(-1) for tributyrin. kcat is 122.9 sec(-1) for tricaproin. kcat is 15.6 sec(-1) for tricaprylin. kcat is 501.8 sec(-1) for p-nitrophenyl acetate. kcat is 119.7 sec(-1) for p-nitrophenyl butyrate. kcat is 33.1 sec(-1) for p-nitrophenyl caproate.
  1. KM=0.13 mM for triacetin1 Publication
  2. KM=0.24 mM for tributyrin1 Publication
  3. KM=0.25 mM for tricaproin1 Publication
  4. KM=1.46 mM for tricaprylin1 Publication
  5. KM=0.16 mM for p-nitrophenyl acetate1 Publication
  6. KM=0.18 mM for p-nitrophenyl butyrate1 Publication
  7. KM=0.58 mM for p-nitrophenyl caproate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 35 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei901 Publication1
    Active sitei1891 Publication1
    Active sitei2191 Publication1

    GO - Molecular functioni

    • carboxylic ester hydrolase activity Source: MTBBASE
    • phospholipase C activity Source: MTBBASE

    GO - Biological processi

    • growth of symbiont in host cell Source: MTBBASE
    • metabolic process Source: InterPro
    • response to acidic pH Source: MTBBASE
    • response to host immune response Source: MTBBASE

    Keywordsi

    Molecular functionHydrolase, Serine esterase
    Biological processStress response

    Protein family/group databases

    ESTHERimyctu-Rv3487c Hormone-sensitive_lipase_like

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxylesterase LipF (EC:3.1.1.1)
    Gene namesi
    Name:lipF
    Ordered Locus Names:Rv3487c
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv3487c

    Pathology & Biotechi

    Disruption phenotypei

    Mutants show reduced ability to grow in a mouse lung.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi90S → A: Loss of activity. 1 Publication1
    Mutagenesisi189E → A: Loss of activity. 1 Publication1
    Mutagenesisi219H → A: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004191681 – 277Carboxylesterase LipFAdd BLAST277

    Proteomic databases

    PaxDbiO06350
    PRIDEiO06350

    Expressioni

    Inductioni

    Induced by acidic pH.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi83332.Rv3487c

    Structurei

    3D structure databases

    ProteinModelPortaliO06350
    SMRiO06350
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi20 – 22Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity3

    Sequence similaritiesi

    Belongs to the 'GDXG' lipolytic enzyme family.Curated

    Phylogenomic databases

    HOGENOMiHOG000152319
    InParanoidiO06350
    OMAiMVAFINA
    PhylomeDBiO06350

    Family and domain databases

    Gene3Di3.40.50.1820, 1 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR013094 AB_hydrolase_3
    IPR033140 Lipase_GDXG_put_SER_AS
    PfamiView protein in Pfam
    PF07859 Abhydrolase_3, 1 hit
    SUPFAMiSSF53474 SSF53474, 1 hit
    PROSITEiView protein in PROSITE
    PS01174 LIPASE_GDXG_SER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O06350-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRAPGVRAAD GAGRVVLYLH GGAFVMCGPN SHSRIVNALS GFAESPVLIV
    60 70 80 90 100
    DYRLIPKHSL GMALDDCHDA YQWLRARGYR PEQIVLAGDS AGGYLALALA
    110 120 130 140 150
    QRLQCDDEKP AAIVAISPLL QLAKGPKQDH PNIGTDAMFP ARAFDALAAW
    160 170 180 190 200
    VRAAAAKNMV DGRPEDLYEP LDHIESSLPP TLIHVSGSEV LLHDAQLGAG
    210 220 230 240 250
    KLAAAGVCAE VRVWPGQAHL FQLATPLVPE ATRSLRQIGQ FIRDATADSS
    260 270
    LSPVHRSRYV AGSPRAASRG AFGQSPI
    Length:277
    Mass (Da):29,430
    Last modified:July 5, 2004 - v3
    Checksum:i79E38E7E4F8DE54D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP46309.1
    PIRiD70569
    RefSeqiNP_218004.3, NC_000962.3
    WP_003418946.1, NZ_KK339370.1

    Genome annotation databases

    EnsemblBacteriaiCCP46309; CCP46309; Rv3487c
    GeneIDi888430
    KEGGimtu:Rv3487c

    Similar proteinsi

    Entry informationi

    Entry nameiLIPF_MYCTU
    AccessioniPrimary (citable) accession number: O06350
    Secondary accession number(s): F2GJ51, L0TCW1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: July 5, 2004
    Last modified: May 23, 2018
    This is version 109 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families
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    Main funding by: National Institutes of Health