Hypoxic response protein 1 - Mycobacterium tuberculosis

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O06186 (HRP1_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hypoxic response protein 1
Short name=HRP1

Gene names

Ordered Locus Names:

Rv2626c, MT2701

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Unlike some other CBS-domain containing proteins does not seem to bind AMP.
Subunit structure	Homodimer. Forms an SDS-resistant dimer that requires Cys-136 for SDS-resistance. Ref.8 Ref.12
Subcellular location	Secreted. Note: Not seen to be associated with the cell wall. Ref.12
Induction	A member of the dormancy regulon. Induced in response to reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and carbon monoxide (CO). It is hoped that this regulon will give insight into the latent, or dormant phase of infection. Following a shift from stationary to anaerobic growth this protein is not seen to not be further induced (at protein level). Induced in mouse lungs at the same time that adaptive host immunity induces bacterial growth arrest; induction is dependent on interferon gamma. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10 Ref.11
Biotechnological use	In plasmid DNA-vaccinated mice, subsequent challenge with this protein induces positive levels of antigen-specific IFN-gamma and IL-2, indicating this might be a good vaccine candidate. Ref.9
Miscellaneous	Has been detected extracellularly but no signal sequence is predicted by bioinformatic programs.
Sequence similarities	Contains 2 CBS domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	CBS domain Repeat
Ligand	Metal-binding Zinc
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to host immune response Inferred from expression pattern. Source: MTBBASE response to hypoxia Inferred from direct assay Ref.4. Source: MTBBASE
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 143

143

Hypoxic response protein 1

PRO_0000392622

Regions

Domain

8 – 65

CBS 1

Domain

73 – 131

CBS 2

Sites

Metal binding

Zinc 1

Metal binding

122

Zinc 2

Amino acid modifications

Disulfide bond

14 ↔ 39

Disulfide bond

136

Interchain

Experimental info

Mutagenesis

C → A: Forms less stable dimer. Ref.12

Mutagenesis

C → A: Forms less stable dimer. Ref.12

Mutagenesis

128 – 143

Missing: No longer forms SDS-resistant dimer.

Mutagenesis

136

C → A: No longer forms SDS-resistant dimer. Ref.12

Secondary structure

143

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O06186 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: CF65AA9144341038
Show »

FASTA

143

15,518

        10         20         30         40         50         60 
MTTARDIMNA GVTCVGEHET LTAAAQYMRE HDIGALPICG DDDRLHGMLT DRDIVIKGLA 

        70         80         90        100        110        120 
AGLDPNTATA GELARDSIYY VDANASIQEM LNVMEEHQVR RVPVISEHRL VGIVTEADIA 

       130        140 
RHLPEHAIVQ FVKAICSPMA LAS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin." Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I., Schoolnik G.K. Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001) [PubMed: 11416222] [Abstract] Cited for: INDUCTION BY HYPOXIA. Strain: ATCC 25618 / H37Rv.
[4]	"Hypoxic response of Mycobacterium tuberculosis studied by metabolic labeling and proteome analysis of cellular and extracellular proteins." Rosenkrands I., Slayden R.A., Crawford J., Aagaard C., Barry C.E. III, Andersen P. J. Bacteriol. 184:3485-3491(2002) [PubMed: 12057942] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY HYPOXIA. Strain: ATCC 25618 / H37Rv.
[5]	"Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program." Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M., Sherman D.R., Schoolnik G.K. J. Exp. Med. 198:705-713(2003) [PubMed: 12953092] [Abstract] Cited for: INDUCTION BY NITRIC OXIDE (NO), BY HYPOXIA, IN MOUSE MODEL, DORMANCY REGULON. Strain: 1254, ATCC 25618 / H37Rv and CDC 1551 / Oshkosh.
[6]	"Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistence." Shi L., Jung Y.J., Tyagi S., Gennaro M.L., North R.J. Proc. Natl. Acad. Sci. U.S.A. 100:241-246(2003) [PubMed: 12506197] [Abstract] Cited for: INDUCTION BY HOST IMMUNITY. Strain: ATCC 25618 / H37Rv.
[7]	"Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions." Starck J., Kallenius G., Marklund B.I., Andersson D.I., Akerlund T. Microbiology 150:3821-3829(2004) [PubMed: 15528667] [Abstract] Cited for: STATIONARY PHASE INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY. Strain: S-02293 Harlingen.
[8]	"Crystallization of a protein using dehydration without a precipitant." Sharpe M.L., Baker E.N., Lott J.S. Acta Crystallogr. F 61:565-568(2005) [PubMed: 16511097] [Abstract] Cited for: SUBUNIT, PRELIMINARY CRYSTALLOGRAPHY. Strain: ATCC 25618 / H37Rv.
[9]	"Immunogenicity of eight dormancy regulon-encoded proteins of Mycobacterium tuberculosis in DNA-vaccinated and tuberculosis-infected mice." Roupie V., Romano M., Zhang L., Korf H., Lin M.Y., Franken K.L., Ottenhoff T.H., Klein M.R., Huygen K. Infect. Immun. 75:941-949(2007) [PubMed: 17145953] [Abstract] Cited for: BIOTECHNOLOGY. Strain: ATCC 25618 / H37Rv.
[10]	"Mycobacterium tuberculosis senses host-derived carbon monoxide during macrophage infection." Shiloh M.U., Manzanillo P., Cox J.S. Cell Host Microbe 3:323-330(2008) [PubMed: 18474359] [Abstract] Cited for: INDUCTION BY CARBON MONOXIDE (CO). Strain: ATCC 35801 / TMC 107 / Erdman.
[11]	"Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium tuberculosis dormancy regulon." Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I., Agarwal A., Steyn A.J. J. Biol. Chem. 283:18032-18039(2008) [PubMed: 18400743] [Abstract] Cited for: INDUCTION BY CARBON MONOXIDE (CO), DORMANCY REGULON. Strain: ATCC 25618 / H37Rv.
[12]	"The structure and unusual protein chemistry of hypoxic response protein 1, a latency antigen and highly expressed member of the DosR regulon in Mycobacterium tuberculosis." Sharpe M.L., Gao C., Kendall S.L., Baker E.N., Lott J.S. J. Mol. Biol. 383:822-836(2008) [PubMed: 18640126] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 1-127, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-14; CYS-39 AND CYS-136. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000516 Genomic DNA. Translation: AAK47017.1.
BX842580 Genomic DNA. Translation: CAB08616.1.

PIR

A70573.

RefSeq

NP_217142.1. NC_000962.2.
NP_337203.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XKF	X-ray	1.90	A/B	1-127	[»]
1Y5H	X-ray	1.50	A/B	1-127	[»]

ProteinModelPortal

O06186.

SMR

O06186. Positions 2-124.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000001242; EBMYCP00000001242; EBMYCG00000001242.
EBMYCT00000072169; EBMYCP00000070228; EBMYCG00000072164.

GeneID

888576.
925590.

GenomeReviews

Gene locus MT2701 in contig AE000516_GR.
Gene locus Rv2626c in contig AL123456_GR.

KEGG

mtc:MT2701.
mtu:Rv2626c.

PATRIC

18127682. VBIMycTub22151_2944.

TIGR

MT2701.

Organism-specific databases

TubercuList

Rv2626c.

Phylogenomic databases

GeneTree

EBGT00050000017423.

HOGENOM

HBG739441.

OMA

PDAKVRD.

PhylomeDB

O06186.

ProtClustDB

CLSK791962.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR000644. Cysta_beta_synth_core.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF00571. CBS. 2 hits.
[Graphical view]

SMART

SM00116. CBS. 2 hits.
[Graphical view]

PROSITE

PS51371. CBS. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HRP1_MYCTU

Accession

Primary (citable) accession number: O06186
Secondary accession number(s): Q7D6V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents