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O05974 (DPO3A_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha

EC=2.7.7.7
Gene names
Name:dnaE
Ordered Locus Names:RP778
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length1182 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3'-5' exonuclease activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11821182DNA polymerase III subunit alpha
PRO_0000103336

Experimental info

Sequence conflict3761I → V in CAA72479. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O05974 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 0FE9B56969C2E52A

FASTA1,182133,463
        10         20         30         40         50         60 
MQPEFIHLRT QSSYSFLESA LTIEKVVELA LLHKMPALCL SDRGNLFGSL EFSLYAVKKK 

        70         80         90        100        110        120 
LQPIHGVILN IQYDINAFAQ ILLIAKDETG YKNLLKLSSL TFTKNDRKIC EHIGFEDLIK 

       130        140        150        160        170        180 
YQEGVIALCC YTDGIVGKCL LARKQEQAIL FARRLQAILG DRFYFEIMRH DLPEEQFIEN 

       190        200        210        220        230        240 
SYIQIASELS IPIVATNKVL FSEKSMHYAH DVLLCISEGV TKEYPDRKTV SENCYFKSPA 

       250        260        270        280        290        300 
EMRKLFSDLP NAIQNTINLR ERCYFAAHPN PPMLPNFSTQ DISETYLIRK YATEGLLARL 

       310        320        330        340        350        360 
VTKFKAENIS LEHQEKLKTE YFTRLNYELD IICNMNFAGY FLIVSDFIKW SKKQGILVGP 

       370        380        390        400        410        420 
GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP DFDIDFCQER REEVINYVRS 

       430        440        450        460        470        480 
KYGPNRVGQI ITFGKMQAKA VIKDVARVLS LPYKLADYLT ELVPFSAINP VSLEQAIREV 

       490        500        510        520        530        540 
PELANAAKGN GLYNLEGDAE LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY 

       550        560        570        580        590        600 
KDANADMLIV GYSMKYSEIA GLIKFDFLGL QTLTVITNCK KLLKEQGIKI DFDDMTFDDK 

       610        620        630        640        650        660 
KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME NIPTYIACKH 

       670        680        690        700        710        720 
KLQQPDYLHK LLEPILEETY GVVIYQEQVQ RIAQVLAGYT LGAADLLRRA MGKKIKKEME 

       730        740        750        760        770        780 
EQEEIFVKGA IANNISPSQA KSIFSTVAKF AGYGFNKAHA AAYGVISYQT AYLKANYPAE 

       790        800        810        820        830        840 
FLVACLNLEL NNHDKINLFL QEAKDNGIKI IAPNINISEG YFSVKSSNTV ITHSTKSVIS 

       850        860        870        880        890        900 
RLNCDIKKIA KDTAVKPLYC KDESTIIFAL GAIKGVTANF GKLVTDERKA RGAFKSITDF 

       910        920        930        940        950        960 
IERLPPKSIN SKLLENLIKA GCFDELHDNR LQLFLSIPKL IAYSTSYHQE QESNQFSLIK 

       970        980        990       1000       1010       1020 
VSSLSPTILV SSDYADKNTL AFYEFEAMGL FLSNHPLTEY QGIFSRLNIL NTRDLYNKLP 

      1030       1040       1050       1060       1070       1080 
NGTNRVTLAG VIQKKDSRMS ARGRFVTLVL SDPENIFELT IFSEEVLKDY VHLLDVKSLV 

      1090       1100       1110       1120       1130       1140 
VVNCDVVKDE GGIKLTAKSF LSIEDVMNNR QFELQLYPQN YAELEQIITL LASRTSNGYQ 

      1150       1160       1170       1180 
SNAKATIYLQ SKDVKHFIAK ITLSETFFLQ VQDFEVLNQY IR 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.
[2]"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
Andersson J.O., Andersson S.G.E.
Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-780.
Strain: Madrid E.
[3]"Genome degradation is an ongoing process in Rickettsia."
Andersson J.O., Andersson S.G.E.
Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 822-1182.
Strain: B and Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235273 Genomic DNA. Translation: CAA15204.1.
Y11785 Genomic DNA. Translation: CAA72479.1.
AJ238755 Genomic DNA. Translation: CAB56087.1.
AJ238756 Genomic DNA. Translation: CAB56091.1.
PIRD71638.
RefSeqNP_221128.1. NC_000963.1.

3D structure databases

ProteinModelPortalO05974.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP778.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA15204; CAA15204; CAA15204.
GeneID883774.
KEGGrpr:RP778.
PATRIC17902424. VBIRicPro72556_0814.

Phylogenomic databases

eggNOGCOG0587.
HOGENOMHOG000021784.
KOK02337.
OMAFCMDGRD.
OrthoDBEOG6CZQGR.
ProtClustDBPRK05673.

Family and domain databases

InterProIPR011708. DNA_pol3_alpha.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. SSF89550. 1 hit.
TIGRFAMsTIGR00594. polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPO3A_RICPR
AccessionPrimary (citable) accession number: O05974
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names