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Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: InterPro
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Ordered Locus Names:RP778
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11821182DNA polymerase III subunit alphaPRO_0000103336Add
BLAST

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi272947.RP778.

Structurei

3D structure databases

ProteinModelPortaliO05974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
KOiK02337.
OMAiMKFPGYM.
OrthoDBiEOG6CZQGR.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

O05974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPEFIHLRT QSSYSFLESA LTIEKVVELA LLHKMPALCL SDRGNLFGSL
60 70 80 90 100
EFSLYAVKKK LQPIHGVILN IQYDINAFAQ ILLIAKDETG YKNLLKLSSL
110 120 130 140 150
TFTKNDRKIC EHIGFEDLIK YQEGVIALCC YTDGIVGKCL LARKQEQAIL
160 170 180 190 200
FARRLQAILG DRFYFEIMRH DLPEEQFIEN SYIQIASELS IPIVATNKVL
210 220 230 240 250
FSEKSMHYAH DVLLCISEGV TKEYPDRKTV SENCYFKSPA EMRKLFSDLP
260 270 280 290 300
NAIQNTINLR ERCYFAAHPN PPMLPNFSTQ DISETYLIRK YATEGLLARL
310 320 330 340 350
VTKFKAENIS LEHQEKLKTE YFTRLNYELD IICNMNFAGY FLIVSDFIKW
360 370 380 390 400
SKKQGILVGP GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP
410 420 430 440 450
DFDIDFCQER REEVINYVRS KYGPNRVGQI ITFGKMQAKA VIKDVARVLS
460 470 480 490 500
LPYKLADYLT ELVPFSAINP VSLEQAIREV PELANAAKGN GLYNLEGDAE
510 520 530 540 550
LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY KDANADMLIV
560 570 580 590 600
GYSMKYSEIA GLIKFDFLGL QTLTVITNCK KLLKEQGIKI DFDDMTFDDK
610 620 630 640 650
KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME
660 670 680 690 700
NIPTYIACKH KLQQPDYLHK LLEPILEETY GVVIYQEQVQ RIAQVLAGYT
710 720 730 740 750
LGAADLLRRA MGKKIKKEME EQEEIFVKGA IANNISPSQA KSIFSTVAKF
760 770 780 790 800
AGYGFNKAHA AAYGVISYQT AYLKANYPAE FLVACLNLEL NNHDKINLFL
810 820 830 840 850
QEAKDNGIKI IAPNINISEG YFSVKSSNTV ITHSTKSVIS RLNCDIKKIA
860 870 880 890 900
KDTAVKPLYC KDESTIIFAL GAIKGVTANF GKLVTDERKA RGAFKSITDF
910 920 930 940 950
IERLPPKSIN SKLLENLIKA GCFDELHDNR LQLFLSIPKL IAYSTSYHQE
960 970 980 990 1000
QESNQFSLIK VSSLSPTILV SSDYADKNTL AFYEFEAMGL FLSNHPLTEY
1010 1020 1030 1040 1050
QGIFSRLNIL NTRDLYNKLP NGTNRVTLAG VIQKKDSRMS ARGRFVTLVL
1060 1070 1080 1090 1100
SDPENIFELT IFSEEVLKDY VHLLDVKSLV VVNCDVVKDE GGIKLTAKSF
1110 1120 1130 1140 1150
LSIEDVMNNR QFELQLYPQN YAELEQIITL LASRTSNGYQ SNAKATIYLQ
1160 1170 1180
SKDVKHFIAK ITLSETFFLQ VQDFEVLNQY IR
Length:1,182
Mass (Da):133,463
Last modified:May 30, 2000 - v2
Checksum:i0FE9B56969C2E52A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti376 – 3761I → V in CAA72479 (PubMed:9274032).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15204.1.
Y11785 Genomic DNA. Translation: CAA72479.1.
AJ238755 Genomic DNA. Translation: CAB56087.1.
AJ238756 Genomic DNA. Translation: CAB56091.1.
PIRiD71638.
RefSeqiNP_221128.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15204; CAA15204; CAA15204.
GeneIDi883774.
KEGGirpr:RP778.
PATRICi17902424. VBIRicPro72556_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15204.1.
Y11785 Genomic DNA. Translation: CAA72479.1.
AJ238755 Genomic DNA. Translation: CAB56087.1.
AJ238756 Genomic DNA. Translation: CAB56091.1.
PIRiD71638.
RefSeqiNP_221128.1. NC_000963.1.

3D structure databases

ProteinModelPortaliO05974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15204; CAA15204; CAA15204.
GeneIDi883774.
KEGGirpr:RP778.
PATRICi17902424. VBIRicPro72556_0814.

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
KOiK02337.
OMAiMKFPGYM.
OrthoDBiEOG6CZQGR.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  2. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
    Andersson J.O., Andersson S.G.E.
    Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-780.
    Strain: Madrid E.
  3. "Genome degradation is an ongoing process in Rickettsia."
    Andersson J.O., Andersson S.G.E.
    Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 822-1182.
    Strain: B and Madrid E.

Entry informationi

Entry nameiDPO3A_RICPR
AccessioniPrimary (citable) accession number: O05974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.