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O05974

- DPO3A_RICPR

UniProt

O05974 - DPO3A_RICPR

Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: InterPro
    2. DNA binding Source: InterPro
    3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit alpha (EC:2.7.7.7)
    Gene namesi
    Name:dnaE
    Ordered Locus Names:RP778
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11821182DNA polymerase III subunit alphaPRO_0000103336Add
    BLAST

    Interactioni

    Subunit structurei

    DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.By similarity

    Protein-protein interaction databases

    STRINGi272947.RP778.

    Structurei

    3D structure databases

    ProteinModelPortaliO05974.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0587.
    HOGENOMiHOG000021784.
    KOiK02337.
    OMAiFCMDGRD.
    OrthoDBiEOG6CZQGR.

    Family and domain databases

    InterProiIPR011708. DNA_pol3_alpha.
    IPR029460. DNAPol_HHH.
    IPR004365. NA-bd_OB_tRNA.
    IPR004013. PHP_C.
    IPR003141. Pol/His_phosphatase_N.
    IPR016195. Pol/histidinol_Pase-like.
    IPR004805. PolC_alpha.
    [Graphical view]
    PfamiPF07733. DNA_pol3_alpha. 1 hit.
    PF14579. HHH_6. 1 hit.
    PF02811. PHP. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    SMARTiSM00481. POLIIIAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF89550. SSF89550. 1 hit.
    TIGRFAMsiTIGR00594. polc. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O05974-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPEFIHLRT QSSYSFLESA LTIEKVVELA LLHKMPALCL SDRGNLFGSL     50
    EFSLYAVKKK LQPIHGVILN IQYDINAFAQ ILLIAKDETG YKNLLKLSSL 100
    TFTKNDRKIC EHIGFEDLIK YQEGVIALCC YTDGIVGKCL LARKQEQAIL 150
    FARRLQAILG DRFYFEIMRH DLPEEQFIEN SYIQIASELS IPIVATNKVL 200
    FSEKSMHYAH DVLLCISEGV TKEYPDRKTV SENCYFKSPA EMRKLFSDLP 250
    NAIQNTINLR ERCYFAAHPN PPMLPNFSTQ DISETYLIRK YATEGLLARL 300
    VTKFKAENIS LEHQEKLKTE YFTRLNYELD IICNMNFAGY FLIVSDFIKW 350
    SKKQGILVGP GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP 400
    DFDIDFCQER REEVINYVRS KYGPNRVGQI ITFGKMQAKA VIKDVARVLS 450
    LPYKLADYLT ELVPFSAINP VSLEQAIREV PELANAAKGN GLYNLEGDAE 500
    LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY KDANADMLIV 550
    GYSMKYSEIA GLIKFDFLGL QTLTVITNCK KLLKEQGIKI DFDDMTFDDK 600
    KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME 650
    NIPTYIACKH KLQQPDYLHK LLEPILEETY GVVIYQEQVQ RIAQVLAGYT 700
    LGAADLLRRA MGKKIKKEME EQEEIFVKGA IANNISPSQA KSIFSTVAKF 750
    AGYGFNKAHA AAYGVISYQT AYLKANYPAE FLVACLNLEL NNHDKINLFL 800
    QEAKDNGIKI IAPNINISEG YFSVKSSNTV ITHSTKSVIS RLNCDIKKIA 850
    KDTAVKPLYC KDESTIIFAL GAIKGVTANF GKLVTDERKA RGAFKSITDF 900
    IERLPPKSIN SKLLENLIKA GCFDELHDNR LQLFLSIPKL IAYSTSYHQE 950
    QESNQFSLIK VSSLSPTILV SSDYADKNTL AFYEFEAMGL FLSNHPLTEY 1000
    QGIFSRLNIL NTRDLYNKLP NGTNRVTLAG VIQKKDSRMS ARGRFVTLVL 1050
    SDPENIFELT IFSEEVLKDY VHLLDVKSLV VVNCDVVKDE GGIKLTAKSF 1100
    LSIEDVMNNR QFELQLYPQN YAELEQIITL LASRTSNGYQ SNAKATIYLQ 1150
    SKDVKHFIAK ITLSETFFLQ VQDFEVLNQY IR 1182
    Length:1,182
    Mass (Da):133,463
    Last modified:May 30, 2000 - v2
    Checksum:i0FE9B56969C2E52A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti376 – 3761I → V in CAA72479. (PubMed:9274032)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15204.1.
    Y11785 Genomic DNA. Translation: CAA72479.1.
    AJ238755 Genomic DNA. Translation: CAB56087.1.
    AJ238756 Genomic DNA. Translation: CAB56091.1.
    PIRiD71638.
    RefSeqiNP_221128.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA15204; CAA15204; CAA15204.
    GeneIDi883774.
    KEGGirpr:RP778.
    PATRICi17902424. VBIRicPro72556_0814.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15204.1 .
    Y11785 Genomic DNA. Translation: CAA72479.1 .
    AJ238755 Genomic DNA. Translation: CAB56087.1 .
    AJ238756 Genomic DNA. Translation: CAB56091.1 .
    PIRi D71638.
    RefSeqi NP_221128.1. NC_000963.1.

    3D structure databases

    ProteinModelPortali O05974.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272947.RP778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA15204 ; CAA15204 ; CAA15204 .
    GeneIDi 883774.
    KEGGi rpr:RP778.
    PATRICi 17902424. VBIRicPro72556_0814.

    Phylogenomic databases

    eggNOGi COG0587.
    HOGENOMi HOG000021784.
    KOi K02337.
    OMAi FCMDGRD.
    OrthoDBi EOG6CZQGR.

    Family and domain databases

    InterProi IPR011708. DNA_pol3_alpha.
    IPR029460. DNAPol_HHH.
    IPR004365. NA-bd_OB_tRNA.
    IPR004013. PHP_C.
    IPR003141. Pol/His_phosphatase_N.
    IPR016195. Pol/histidinol_Pase-like.
    IPR004805. PolC_alpha.
    [Graphical view ]
    Pfami PF07733. DNA_pol3_alpha. 1 hit.
    PF14579. HHH_6. 1 hit.
    PF02811. PHP. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    SMARTi SM00481. POLIIIAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF89550. SSF89550. 1 hit.
    TIGRFAMsi TIGR00594. polc. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.
    2. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
      Andersson J.O., Andersson S.G.E.
      Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-780.
      Strain: Madrid E.
    3. "Genome degradation is an ongoing process in Rickettsia."
      Andersson J.O., Andersson S.G.E.
      Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 822-1182.
      Strain: B and Madrid E.

    Entry informationi

    Entry nameiDPO3A_RICPR
    AccessioniPrimary (citable) accession number: O05974
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3