O05974 (DPO3A_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7

Gene names

Name:	dnaE
Ordered Locus Names:	RP778

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	1182 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1182	1182	DNA polymerase III subunit alpha		PRO_0000103336
Experimental info
	Sequence conflict	376	1	I → V in CAA72479. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O05974 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 0FE9B56969C2E52A	FASTA	1,182	133,463
	10 20 30 40 50 60 MQPEFIHLRT QSSYSFLESA LTIEKVVELA LLHKMPALCL SDRGNLFGSL EFSLYAVKKK 70 80 90 100 110 120 LQPIHGVILN IQYDINAFAQ ILLIAKDETG YKNLLKLSSL TFTKNDRKIC EHIGFEDLIK 130 140 150 160 170 180 YQEGVIALCC YTDGIVGKCL LARKQEQAIL FARRLQAILG DRFYFEIMRH DLPEEQFIEN 190 200 210 220 230 240 SYIQIASELS IPIVATNKVL FSEKSMHYAH DVLLCISEGV TKEYPDRKTV SENCYFKSPA 250 260 270 280 290 300 EMRKLFSDLP NAIQNTINLR ERCYFAAHPN PPMLPNFSTQ DISETYLIRK YATEGLLARL 310 320 330 340 350 360 VTKFKAENIS LEHQEKLKTE YFTRLNYELD IICNMNFAGY FLIVSDFIKW SKKQGILVGP 370 380 390 400 410 420 GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP DFDIDFCQER REEVINYVRS 430 440 450 460 470 480 KYGPNRVGQI ITFGKMQAKA VIKDVARVLS LPYKLADYLT ELVPFSAINP VSLEQAIREV 490 500 510 520 530 540 PELANAAKGN GLYNLEGDAE LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY 550 560 570 580 590 600 KDANADMLIV GYSMKYSEIA GLIKFDFLGL QTLTVITNCK KLLKEQGIKI DFDDMTFDDK 610 620 630 640 650 660 KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME NIPTYIACKH 670 680 690 700 710 720 KLQQPDYLHK LLEPILEETY GVVIYQEQVQ RIAQVLAGYT LGAADLLRRA MGKKIKKEME 730 740 750 760 770 780 EQEEIFVKGA IANNISPSQA KSIFSTVAKF AGYGFNKAHA AAYGVISYQT AYLKANYPAE 790 800 810 820 830 840 FLVACLNLEL NNHDKINLFL QEAKDNGIKI IAPNINISEG YFSVKSSNTV ITHSTKSVIS 850 860 870 880 890 900 RLNCDIKKIA KDTAVKPLYC KDESTIIFAL GAIKGVTANF GKLVTDERKA RGAFKSITDF 910 920 930 940 950 960 IERLPPKSIN SKLLENLIKA GCFDELHDNR LQLFLSIPKL IAYSTSYHQE QESNQFSLIK 970 980 990 1000 1010 1020 VSSLSPTILV SSDYADKNTL AFYEFEAMGL FLSNHPLTEY QGIFSRLNIL NTRDLYNKLP 1030 1040 1050 1060 1070 1080 NGTNRVTLAG VIQKKDSRMS ARGRFVTLVL SDPENIFELT IFSEEVLKDY VHLLDVKSLV 1090 1100 1110 1120 1130 1140 VVNCDVVKDE GGIKLTAKSF LSIEDVMNNR QFELQLYPQN YAELEQIITL LASRTSNGYQ 1150 1160 1170 1180 SNAKATIYLQ SKDVKHFIAK ITLSETFFLQ VQDFEVLNQY IR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence." Andersson J.O., Andersson S.G.E. Microbiology 143:2783-2795(1997) [PubMed: 9274032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-780. Strain: Madrid E.
[3]	"Genome degradation is an ongoing process in Rickettsia." Andersson J.O., Andersson S.G.E. Mol. Biol. Evol. 16:1178-1191(1999) [PubMed: 10486973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 822-1182. Strain: B and Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15204.1. Y11785 Genomic DNA. Translation: CAA72479.1. AJ238755 Genomic DNA. Translation: CAB56087.1. AJ238756 Genomic DNA. Translation: CAB56091.1.
PIR	D71638.
RefSeq	NP_221128.1. NC_000963.1.
3D structure databases
ProteinModelPortal	O05974.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883774.
GenomeReviews	Gene locus RP778 in contig AJ235269_GR.
KEGG	rpr:RP778.
PATRIC	17902424. VBIRicPro72556_0814.
Phylogenomic databases
HOGENOM	HBG734490.
OMA	RVLQMPY.
ProtClustDB	PRK05673.
Enzyme and pathway databases
BioCyc	RPRO272947:RP778-MONOMER.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K02337.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3A_RICPR

Accession

Primary (citable) accession number: O05974

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program