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O05974

- DPO3A_RICPR

UniProt

O05974 - DPO3A_RICPR

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Protein

DNA polymerase III subunit alpha

Gene
dnaE, RP778
Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: InterPro
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Ordered Locus Names:RP778
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11821182DNA polymerase III subunit alphaPRO_0000103336Add
BLAST

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Protein-protein interaction databases

STRINGi272947.RP778.

Structurei

3D structure databases

ProteinModelPortaliO05974.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
KOiK02337.
OMAiFCMDGRD.
OrthoDBiEOG6CZQGR.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

O05974-1 [UniParc]FASTAAdd to Basket

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MQPEFIHLRT QSSYSFLESA LTIEKVVELA LLHKMPALCL SDRGNLFGSL     50
EFSLYAVKKK LQPIHGVILN IQYDINAFAQ ILLIAKDETG YKNLLKLSSL 100
TFTKNDRKIC EHIGFEDLIK YQEGVIALCC YTDGIVGKCL LARKQEQAIL 150
FARRLQAILG DRFYFEIMRH DLPEEQFIEN SYIQIASELS IPIVATNKVL 200
FSEKSMHYAH DVLLCISEGV TKEYPDRKTV SENCYFKSPA EMRKLFSDLP 250
NAIQNTINLR ERCYFAAHPN PPMLPNFSTQ DISETYLIRK YATEGLLARL 300
VTKFKAENIS LEHQEKLKTE YFTRLNYELD IICNMNFAGY FLIVSDFIKW 350
SKKQGILVGP GRGSGAGSVV AWSLLITDLD PIKFGLLFER FLNPERISMP 400
DFDIDFCQER REEVINYVRS KYGPNRVGQI ITFGKMQAKA VIKDVARVLS 450
LPYKLADYLT ELVPFSAINP VSLEQAIREV PELANAAKGN GLYNLEGDAE 500
LIKLVLDTSL ILEGLHRHSS THAAGIVIAG TDLVDIVPVY KDANADMLIV 550
GYSMKYSEIA GLIKFDFLGL QTLTVITNCK KLLKEQGIKI DFDDMTFDDK 600
KTYQMLCKGK GVGVFQFESI GMKDALRRLK PDSIHDLIAL GALYRPGPME 650
NIPTYIACKH KLQQPDYLHK LLEPILEETY GVVIYQEQVQ RIAQVLAGYT 700
LGAADLLRRA MGKKIKKEME EQEEIFVKGA IANNISPSQA KSIFSTVAKF 750
AGYGFNKAHA AAYGVISYQT AYLKANYPAE FLVACLNLEL NNHDKINLFL 800
QEAKDNGIKI IAPNINISEG YFSVKSSNTV ITHSTKSVIS RLNCDIKKIA 850
KDTAVKPLYC KDESTIIFAL GAIKGVTANF GKLVTDERKA RGAFKSITDF 900
IERLPPKSIN SKLLENLIKA GCFDELHDNR LQLFLSIPKL IAYSTSYHQE 950
QESNQFSLIK VSSLSPTILV SSDYADKNTL AFYEFEAMGL FLSNHPLTEY 1000
QGIFSRLNIL NTRDLYNKLP NGTNRVTLAG VIQKKDSRMS ARGRFVTLVL 1050
SDPENIFELT IFSEEVLKDY VHLLDVKSLV VVNCDVVKDE GGIKLTAKSF 1100
LSIEDVMNNR QFELQLYPQN YAELEQIITL LASRTSNGYQ SNAKATIYLQ 1150
SKDVKHFIAK ITLSETFFLQ VQDFEVLNQY IR 1182
Length:1,182
Mass (Da):133,463
Last modified:May 30, 2000 - v2
Checksum:i0FE9B56969C2E52A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti376 – 3761I → V in CAA72479. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235273 Genomic DNA. Translation: CAA15204.1.
Y11785 Genomic DNA. Translation: CAA72479.1.
AJ238755 Genomic DNA. Translation: CAB56087.1.
AJ238756 Genomic DNA. Translation: CAB56091.1.
PIRiD71638.
RefSeqiNP_221128.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15204; CAA15204; CAA15204.
GeneIDi883774.
KEGGirpr:RP778.
PATRICi17902424. VBIRicPro72556_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235273 Genomic DNA. Translation: CAA15204.1 .
Y11785 Genomic DNA. Translation: CAA72479.1 .
AJ238755 Genomic DNA. Translation: CAB56087.1 .
AJ238756 Genomic DNA. Translation: CAB56091.1 .
PIRi D71638.
RefSeqi NP_221128.1. NC_000963.1.

3D structure databases

ProteinModelPortali O05974.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP778.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA15204 ; CAA15204 ; CAA15204 .
GeneIDi 883774.
KEGGi rpr:RP778.
PATRICi 17902424. VBIRicPro72556_0814.

Phylogenomic databases

eggNOGi COG0587.
HOGENOMi HOG000021784.
KOi K02337.
OMAi FCMDGRD.
OrthoDBi EOG6CZQGR.

Family and domain databases

InterProi IPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view ]
Pfami PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
SMARTi SM00481. POLIIIAc. 1 hit.
[Graphical view ]
SUPFAMi SSF89550. SSF89550. 1 hit.
TIGRFAMsi TIGR00594. polc. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  2. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
    Andersson J.O., Andersson S.G.E.
    Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-780.
    Strain: Madrid E.
  3. "Genome degradation is an ongoing process in Rickettsia."
    Andersson J.O., Andersson S.G.E.
    Mol. Biol. Evol. 16:1178-1191(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 822-1182.
    Strain: B and Madrid E.

Entry informationi

Entry nameiDPO3A_RICPR
AccessioniPrimary (citable) accession number: O05974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi