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O05969

- DAPA_RICPR

UniProt

O05969 - DAPA_RICPR

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Protein
4-hydroxy-tetrahydrodipicolinate synthase
Gene
dapA, RP429
Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 461Part of a proton relay during catalysis By similarity
Binding sitei47 – 471Pyruvate By similarity
Sitei109 – 1091Part of a proton relay during catalysis By similarity
Active sitei135 – 1351Proton donor/acceptor By similarity
Active sitei163 – 1631Schiff-base intermediate with substrate By similarity
Binding sitei205 – 2051Pyruvate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase (EC:4.3.3.7)
Short name:
HTPA synthase
Gene namesi
Name:dapA
Ordered Locus Names:RP429
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2942944-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation
PRO_0000103146Add
BLAST

Interactioni

Subunit structurei

Homotetramer; dimer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP429.

Structurei

3D structure databases

ProteinModelPortaliO05969.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiMLYSGDD.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05969-1 [UniParc]FASTAAdd to Basket

« Hide

MYNIFKGLIT ALITPFKDNK LDLYALENIL NQQIKHKIDA VLIAGSTGEA    50
NSLSFEEYKL LLKTSVDIVN NRMPIISGCS SNNTAYAIEL AIESKKIGVD 100
CFMASPPSYV KPTQHGIYKH FEALHEACNL PIMLYSAPTR SGVDFSDETI 150
LRLSKLPRIL ALKDCGVDLE RPMRIRAIVK GDFNILTGND EVVLAFHAQG 200
VVGWISVTSN IAPKLCKELL EKWYNNDTQG ALEIHQKLLP LYKALFLESN 250
PIPVKYAAYY LGLCENEIRL PLTEASDSAK KQIKKIITSL SIKI 294
Length:294
Mass (Da):32,787
Last modified:July 1, 1997 - v1
Checksum:i33A84A526CC7A32E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11777 Genomic DNA. Translation: CAA72449.1.
AJ235271 Genomic DNA. Translation: CAA14886.1.
PIRiD71701.
RefSeqiNP_220810.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14886; CAA14886; CAA14886.
GeneIDi883313.
KEGGirpr:RP429.
PATRICi17901639. VBIRicPro72556_0442.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11777 Genomic DNA. Translation: CAA72449.1 .
AJ235271 Genomic DNA. Translation: CAA14886.1 .
PIRi D71701.
RefSeqi NP_220810.1. NC_000963.1.

3D structure databases

ProteinModelPortali O05969.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP429.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA14886 ; CAA14886 ; CAA14886 .
GeneIDi 883313.
KEGGi rpr:RP429.
PATRICi 17901639. VBIRicPro72556_0442.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi MLYSGDD.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
    Andersson J.O., Andersson S.G.E.
    Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Madrid E.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiDAPA_RICPR
AccessioniPrimary (citable) accession number: O05969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi