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O05956

- END3_RICPR

UniProt

O05956 - END3_RICPR

Protein

Endonuclease III

Gene

nth

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    3. DNA binding Source: UniProtKB-HAMAP
    4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
    Gene namesi
    Name:nthUniRule annotation
    Ordered Locus Names:RP746
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Endonuclease IIIPRO_0000102221Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272947.RP746.

    Structurei

    3D structure databases

    ProteinModelPortaliO05956.
    SMRiO05956. Positions 7-208.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 12720HhHUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nth/MutY family.UniRule annotation
    Contains 1 HhH domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0177.
    HOGENOMiHOG000252206.
    KOiK10773.
    OMAiDTHIYRV.
    OrthoDBiEOG6H4KC5.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_00942. Nth.
    InterProiIPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view]
    PfamiPF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001435. Nth. 1 hit.
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    TIGRFAMsiTIGR01083. nth. 1 hit.
    PROSITEiPS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O05956-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAQIMNKIF EIFSKNNPKP QTELIYKNDF TLLVAVILSA RATDISVNLA    50
    TKHLFETYNT PEKFLELGEE GLKKYIKSIG LFNSKAKNII ALCQILIKNY 100
    QTSIPNNFKE LVKLPGVGRK TANVVLNCLF AMPTMAVDTH VFRVSKRIGL 150
    AKGNTAAIVE KELLQIIDEK WLTYAHHWLI LHGRYICKAR KPGCNICPIK 200
    EYCEYYINTF SS 212
    Length:212
    Mass (Da):24,174
    Last modified:July 1, 1997 - v1
    Checksum:iA7FDA4F6033A6382
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11778 Genomic DNA. Translation: CAA72458.1.
    AJ235273 Genomic DNA. Translation: CAA15174.1.
    PIRiF71634.
    RefSeqiNP_221098.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA15174; CAA15174; CAA15174.
    GeneIDi883968.
    KEGGirpr:RP746.
    PATRICi17902351. VBIRicPro72556_0779.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11778 Genomic DNA. Translation: CAA72458.1 .
    AJ235273 Genomic DNA. Translation: CAA15174.1 .
    PIRi F71634.
    RefSeqi NP_221098.1. NC_000963.1.

    3D structure databases

    ProteinModelPortali O05956.
    SMRi O05956. Positions 7-208.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272947.RP746.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA15174 ; CAA15174 ; CAA15174 .
    GeneIDi 883968.
    KEGGi rpr:RP746.
    PATRICi 17902351. VBIRicPro72556_0779.

    Phylogenomic databases

    eggNOGi COG0177.
    HOGENOMi HOG000252206.
    KOi K10773.
    OMAi DTHIYRV.
    OrthoDBi EOG6H4KC5.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_00942. Nth.
    InterProi IPR011257. DNA_glycosylase.
    IPR004036. Endonuclease-III-like_CS2.
    IPR004035. Endouclease-III_FeS-bd_BS.
    IPR003651. Endouclease3_FeS-loop_motif.
    IPR003265. HhH-GPD_domain.
    IPR000445. HhH_motif.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    IPR005759. Nth.
    [Graphical view ]
    Pfami PF10576. EndIII_4Fe-2S. 1 hit.
    PF00633. HHH. 1 hit.
    PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001435. Nth. 1 hit.
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00525. FES. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    TIGRFAMsi TIGR01083. nth. 1 hit.
    PROSITEi PS00764. ENDONUCLEASE_III_1. 1 hit.
    PS01155. ENDONUCLEASE_III_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
      Andersson J.O., Andersson S.G.E.
      Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Madrid E.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.

    Entry informationi

    Entry nameiEND3_RICPR
    AccessioniPrimary (citable) accession number: O05956
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3