Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O05956 (END3_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease III

EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:RP746
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family.

Contains 1 HhH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212Endonuclease III HAMAP-Rule MF_00942
PRO_0000102221

Regions

Domain108 – 12720HhH

Sites

Metal binding1871Iron-sulfur (4Fe-4S) By similarity
Metal binding1941Iron-sulfur (4Fe-4S) By similarity
Metal binding1971Iron-sulfur (4Fe-4S) By similarity
Metal binding2031Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O05956 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: A7FDA4F6033A6382

FASTA21224,174
        10         20         30         40         50         60 
MQAQIMNKIF EIFSKNNPKP QTELIYKNDF TLLVAVILSA RATDISVNLA TKHLFETYNT 

        70         80         90        100        110        120 
PEKFLELGEE GLKKYIKSIG LFNSKAKNII ALCQILIKNY QTSIPNNFKE LVKLPGVGRK 

       130        140        150        160        170        180 
TANVVLNCLF AMPTMAVDTH VFRVSKRIGL AKGNTAAIVE KELLQIIDEK WLTYAHHWLI 

       190        200        210 
LHGRYICKAR KPGCNICPIK EYCEYYINTF SS 

« Hide

References

« Hide 'large scale' references
[1]"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
Andersson J.O., Andersson S.G.E.
Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Madrid E.
[2]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y11778 Genomic DNA. Translation: CAA72458.1.
AJ235273 Genomic DNA. Translation: CAA15174.1.
PIRF71634.
RefSeqNP_221098.1. NC_000963.1.

3D structure databases

ProteinModelPortalO05956.
SMRO05956. Positions 7-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA15174; CAA15174; CAA15174.
GeneID883968.
KEGGrpr:RP746.
PATRIC17902351. VBIRicPro72556_0779.

Phylogenomic databases

eggNOGCOG0177.
HOGENOMHOG000252206.
KOK10773.
OMANNKSKHL.
OrthoDBEOG6H4KC5.
ProtClustDBCLSK871058.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_RICPR
AccessionPrimary (citable) accession number: O05956
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names