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O05954 (MURE_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Ordered Locus Names:RP597
OrganismRickettsia prowazekii (strain Madrid E)
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity HAMAP MF_00208.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_0000101934

Regions

Nucleotide binding98 – 1047ATP Potential
Region144 – 1452UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region396 – 3994Meso-diaminopimelate binding By similarity
Motif396 – 3994Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site211UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1711UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1771UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1791UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3721Meso-diaminopimelate By similarity
Binding site4461Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4501Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2111N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O05954 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 5864289ED6B5064D

FASTA48053,809
        10         20         30         40         50         60 
MKYNLNQLFK NHRIKGLSTN SKTVKEDEVF FAIKGQNVDG NDFINDALNN GAVLVITENK 

        70         80         90        100        110        120 
NNTAIDKVIY VEDVHKALYE AIEIFYPKKP KNLISVTGTN GKSSVVSYIA QAYSLLKKKA 

       130        140        150        160        170        180 
AFIGTIGLEI FGSNNIINDV PSLTTFDYLS FRKVAHNLAE DSIEYLAFEA SSHGLEQGRL 

       190        200        210        220        230        240 
GKTKVNIVSF TSFSQDHLDY HHTKENYLLA KLKLFTDHLL PSGIAILNSD IEEIEFVKDY 

       250        260        270        280        290        300 
LHNNNVKFIT VGKKGDLQIT KITCSLTGQN IDFIFNNIIY NLHTLIIGSF QASNLLIAAL 

       310        320        330        340        350        360 
TLYYTGFKFD EIIEALAKVK PIKGRMERID GTNIFVDYSH TPDSLEKALI ELKNIKLHGS 

       370        380        390        400        410        420 
KLSVIFGCGG DRDKTKRALM GQIAAKLADN VIITDDNPRF EDPKLIRAEI IRGIGTATYT 

       430        440        450        460        470        480 
EIASRAEAIK YGINNLKQDD ILLIAGKGHE TYQIIGDKKL PFDDSEVVRK YLFEISYTRN

« Hide

References

« Hide 'large scale' references
[1]"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
Andersson J.O., Andersson S.G.E.
Microbiology 143:2783-2795(1997) [PubMed: 9274032] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Madrid E.
[2]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed: 9823893] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y11783 Genomic DNA. Translation: CAA72473.1.
AJ235272 Genomic DNA. Translation: CAA15041.1.
PIRG71664.
RefSeqNP_220965.1. NC_000963.1.

3D structure databases

ProteinModelPortalO05954.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID883656.
GenomeReviewsGene locus RP597 in contig AJ235269_GR.
KEGGrpr:RP597.
PATRIC17902009. VBIRicPro72556_0615.

Phylogenomic databases

HOGENOMHBG602753.
OMADGREHIA.
ProtClustDBPRK00139.

Enzyme and pathway databases

BioCycRPRO272947:RP597-MONOMER.
BRENDA2.1.2.5. 2681.

Family and domain databases

HAMAPMF_00208. MurE.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KOK01928.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01085. MurE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_RICPR
AccessionPrimary (citable) accession number: O05954
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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