O05953 (MURF_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
EC=6.3.2.10

Alternative name(s):
D-alanyl-D-alanine-adding enzyme
UDP-MurNAc-pentapeptide synthetase

Gene names

Name:	murF
Ordered Locus Names:	RP596

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Probable UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase		PRO_0000101704
Regions
	Nucleotide binding	106 – 112	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O05953 [UniParc]. Last modified August 1, 1999. Version 2. Checksum: 3FEB8468F825BFDA	FASTA	449	50,673
	10 20 30 40 50 60 MIWNSKTLSI ALGIEISNSV NCNEVQFNSK DVKKGDLFIA LQGNRDGHDY IQDAIDKGAT 70 80 90 100 110 120 AVIVSKQVEI NDKDKIILVN NSFEALQKMA LYKRENSKAK FIAITGSVGK TSTKEALKIL 130 140 150 160 170 180 LQHDFIVFAS RGNFNNYLGL LINLASMADD TEYAIFELGM NHQGEISELV QILKPNIAMI 190 200 210 220 230 240 NNISEAHLEF FHSLEEIAEA KCEIFKNFSK NDIAIINASN NCYNKILSIL KNLSITNIYS 250 260 270 280 290 300 FGHSSKASAK LILYKTLGEQ VHLQYYINNK FIDITIPFIP RHFANNYTGV LLIIDILGKD 310 320 330 340 350 360 IEISAKYLAD IALTKGRGKI INIQNSRVIC DYYNASPQSM KAALEYLKQV PADNKTSIIG 370 380 390 400 410 420 EMLELGWNSQ RLHEELVPYI LDAGCTKVYL VGAYTKYIYD LLPNKISKKF FKNVEELITH 430 440 ITDLFEYSEL ILIKGSRGVK LDKIVDYYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence." Andersson J.O., Andersson S.G.E. Microbiology 143:2783-2795(1997) [PubMed: 9274032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235272 Genomic DNA. Translation: CAA15040.1. Y11783 Genomic DNA. Translation: CAA72472.1.
PIR	F71664.
RefSeq	NP_220964.1. NC_000963.1.
3D structure databases
ProteinModelPortal	O05953.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883732.
GenomeReviews	Gene locus RP596 in contig AJ235269_GR.
KEGG	rpr:RP596.
PATRIC	17902007. VBIRicPro72556_0614.
Phylogenomic databases
HOGENOM	HBG617121.
OMA	ERIMETK.
ProtClustDB	CLSK871006.
Enzyme and pathway databases
BioCyc	RPRO272947:RP596-MONOMER.
Family and domain databases
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01929.
PANTHER	PTHR23135:SF3. MurF. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. MurF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURF_RICPR

Accession

Primary (citable) accession number: O05953

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1999
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program