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O05952 (RSMA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:RP672
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_0000101595

Sites

Binding site231S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site251S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site501S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site721S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
O05952 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: AF2D364DA5093192

FASTA26830,306
        10         20         30         40         50         60 
MLPSIVKHAT SHKINPLKKH GQNFIFDSSL CDKIIRASNV LENSNVIEIG PGIGGLTRSI 

        70         80         90        100        110        120 
LQKNPKSLTV IEIDERCIPL LNEIQEYYPN LNIIKQDVLK INLTDLIYDK VTVISNLPYH 

       130        140        150        160        170        180 
IGTELVIRLL KEVKLITNMT LMLQKEVVER ICAIPSTKAY GRLSVICQIL AKVEKCFNVA 

       190        200        210        220        230        240 
PTAFYPHPKV YSAIVKIIPL ENPPSIALIN KVEQITKLVF AGRRKMIKSS LRNLIPNIHE 

       250        260 
VLTQLKINCN DRAENLTPKD YLRIAMKL 

« Hide

References

« Hide 'large scale' references
[1]"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
Andersson J.O., Andersson S.G.E.
Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Madrid E.
[2]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y11786 Genomic DNA. Translation: CAA72482.1.
AJ235272 Genomic DNA. Translation: CAA15109.1.
PIRC71673.
RefSeqNP_221033.1. NC_000963.1.

3D structure databases

ProteinModelPortalO05952.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP672.

Proteomic databases

PRIDEO05952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA15109; CAA15109; CAA15109.
GeneID883998.
KEGGrpr:RP672.
PATRIC17902175. VBIRicPro72556_0693.

Phylogenomic databases

eggNOGCOG0030.
HOGENOMHOG000227961.
KOK02528.
OMAITSEILF.
OrthoDBEOG66F08Z.
ProtClustDBPRK00274.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_RICPR
AccessionPrimary (citable) accession number: O05952
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names