O05952 (RSMA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase A
EC=2.1.1.182

Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Gene names

Name:	rsmA
Synonyms:	ksgA
Ordered Locus Names:	RP672

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607
Catalytic activity	4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00607.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607		PRO_0000101595
Sites
	Binding site	23	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	25	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	50	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	97	1	S-adenosyl-L-methionine By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05952 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: AF2D364DA5093192	FASTA	268	30,306
	10 20 30 40 50 60 MLPSIVKHAT SHKINPLKKH GQNFIFDSSL CDKIIRASNV LENSNVIEIG PGIGGLTRSI 70 80 90 100 110 120 LQKNPKSLTV IEIDERCIPL LNEIQEYYPN LNIIKQDVLK INLTDLIYDK VTVISNLPYH 130 140 150 160 170 180 IGTELVIRLL KEVKLITNMT LMLQKEVVER ICAIPSTKAY GRLSVICQIL AKVEKCFNVA 190 200 210 220 230 240 PTAFYPHPKV YSAIVKIIPL ENPPSIALIN KVEQITKLVF AGRRKMIKSS LRNLIPNIHE 250 260 VLTQLKINCN DRAENLTPKD YLRIAMKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence." Andersson J.O., Andersson S.G.E. Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11786 Genomic DNA. Translation: CAA72482.1. AJ235272 Genomic DNA. Translation: CAA15109.1.
PIR	C71673.
RefSeq	NP_221033.1. NC_000963.1.
3D structure databases
ProteinModelPortal	O05952.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP672.
Proteomic databases
PRIDE	O05952.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15109; CAA15109; CAA15109.
GeneID	883998.
KEGG	rpr:RP672.
PATRIC	17902175. VBIRicPro72556_0693.
Phylogenomic databases
eggNOG	COG0030.
HOGENOM	HOG000227961.
KO	K02528.
OMA	IANIPYN.
ProtClustDB	PRK00274.
Family and domain databases
Gene3D	1.10.8.100. 1 hit.
HAMAP	MF_00607. 16SrRNA_methyltr_A.
InterPro	IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
PANTHER	PTHR11727. PTHR11727. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMA_RICPR

Accession

Primary (citable) accession number: O05952

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program