O05949 (DPO1_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase I
Short name=POL I
EC=2.7.7.7

Gene names

Name:	polA
Ordered Locus Names:	RP776

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	867 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Single-chain monomer with multiple functions By similarity.
Sequence similarities	Belongs to the DNA polymerase type-A family. Contains 1 5'-3' exonuclease domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5'-3' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 867	867	DNA polymerase I		PRO_0000101251
Regions
	Domain	1 – 283	283	5'-3' exonuclease

Sequences

	Sequence		Length	Mass (Da)
	O05949 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 557194B38FB69BA2	FASTA	867	98,652
	10 20 30 40 50 60 MTKKNTLLLI DGYGFVFRAY YAQKSLSSAK GEPVGALYGF TSMLLKLLSD FKPQYVAIVF 70 80 90 100 110 120 DSGGKNFRHK IYQNYKANRP LPPKDLIAQL PLVRDVASNF KFAILEKNGY EADDIIATFA 130 140 150 160 170 180 TKTVSLGEEV IIISSDKDLL QLMSKNIKIY DPIKCKYITE DNVVTKFGTT PDKLREVMAL 190 200 210 220 230 240 IGDRSDNIPG VPSIGPKTAS SLITKFGSVE NIFNSLDQIS SIKQRKTLQN AREAALISWK 250 260 270 280 290 300 LIGLDSNVDL DFDLNNLKWS PPNSKKLTGF LQEYGFKSLY KRVENLFDIK INDHEEIVDN 310 320 330 340 350 360 KVTEAKEISN ASELANFAKE AERIGIFGIY LLQQKGENRA LILSLQNQSY IIKITNNNYN 370 380 390 400 410 420 IKNNNDWFSH IILNLLTNKS IKKITYSLKH LLKFYANQSH QITAIEDLEL MQYALSAGLV 430 440 450 460 470 480 QKNLFTKTLT KDNIINESAR IVINFISLYK QTLLELQKNK AFRLYREIDL PTCFILDKME 490 500 510 520 530 540 KVGIKVDANY LNRLSDEFGT EILKIEEEIF ALSGTKFNIG SQKQLGEILF KKMQLPSGNT 550 560 570 580 590 600 LAKTSSYSTR AGILKKLSED GYHIATLLLR WRQLTKLKNT YTDSLPKQIN NITKRIHTTF 610 620 630 640 650 660 LQTSTTTGRL SSQEPNLQNV PIRSSDGNKI REAFIAEEGY KLISADYSQI ELRILSHIAN 670 680 690 700 710 720 IDVLKQAFIN KEDIHTQTAC QIFNLKKHEL TSEHRRKAKA INFGIIYGIS AFGLAKQLNV 730 740 750 760 770 780 SNSTASEYIK QYFAEYKGVQ EYMTQTKACA SRNGYVTNFF GRKCFIPLIH DKKLKQFAER 790 800 810 820 830 840 AAINAPIQGT NADIIKIAMI KLDKEIEERK LKTRLILQIH DELLFEVPEI EVEIVIPIIK 850 860 KIMEHSTNMN VPIITEIKAG NNWKEIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence." Andersson J.O., Andersson S.G.E. Microbiology 143:2783-2795(1997) [PubMed: 9274032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[3]	"Genome degradation is an ongoing process in Rickettsia." Andersson J.O., Andersson S.G.E. Mol. Biol. Evol. 16:1178-1191(1999) [PubMed: 10486973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B and Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11784 Genomic DNA. Translation: CAA72476.1. AJ235273 Genomic DNA. Translation: CAA15203.1. AJ238755 Genomic DNA. Translation: CAB56085.1. AJ238756 Genomic DNA. Translation: CAB56089.1.
PIR	C71638.
RefSeq	NP_221127.1. NC_000963.1.
3D structure databases
ProteinModelPortal	O05949.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883808.
GenomeReviews	Gene locus RP776 in contig AJ235269_GR.
KEGG	rpr:RP776.
PATRIC	17902417. VBIRicPro72556_0811.
Phylogenomic databases
HOGENOM	HBG284758.
OMA	PKGAPST.
ProtClustDB	PRK05755.
Enzyme and pathway databases
BioCyc	RPRO272947:RP776-MONOMER.
Family and domain databases
InterPro	IPR020046. 5-3_exonucl_a-hlix_arch_N. IPR020045. 5-3_exonuclease_C. IPR002421. 5-3_exonuclease_N. IPR019760. DNA-dir_DNA_pol_A_CS. IPR001098. DNA-dir_DNA_pol_A_palm_dom. IPR018320. DNA_polymerase_1. IPR002298. DNA_polymerase_A. IPR008918. HhH2. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K02335.
Pfam	PF01367. 5_3_exonuc. 1 hit. PF02739. 5_3_exonuc_N. 1 hit. PF00476. DNA_pol_A. 1 hit. [Graphical view]
PRINTS	PR00868. DNAPOLI.
SMART	SM00475. 53EXOc. 1 hit. SM00279. HhH2. 1 hit. SM00482. POLAc. 1 hit. [Graphical view]
SUPFAM	SSF47807. 5_3_exo_C. 1 hit. SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00593. Pola. 1 hit.
PROSITE	PS00447. DNA_POLYMERASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPO1_RICPR

Accession

Primary (citable) accession number: O05949

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program