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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi333ZincUniRule annotation1
Metal bindingi384Zinc; via tele nitrogenUniRule annotation1
Metal bindingi510Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:RP221
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001010381 – 635Threonine--tRNA ligaseAdd BLAST635

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP221.

Structurei

3D structure databases

ProteinModelPortaliO05947.
SMRiO05947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 533CatalyticUniRule annotationAdd BLAST292

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22. Bacteria.
COG0441. LUCA.
HOGENOMiHOG000003880.
KOiK01868.
OMAiFYYDFAY.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR036621. Anticodon-bd_dom_sf.
IPR012675. Beta-grasp_dom_sf.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
PANTHERiPTHR11451. PTHR11451. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiView protein in SMART
SM00863. tRNA_SAD. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

O05947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINISFPDGS VKQFAQNITA FEIVNAISMS LAKAAIVVEI NGELKDLSTV
60 70 80 90 100
IENDCKLRIL TAQDYECLEI IRHDAAHLTA EAVKELFPET QVTIGPAIEN
110 120 130 140 150
GYYYDFARDK PFSIDDLANI EAKMQELSKK NEKITRELWD RDKAVEFFLS
160 170 180 190 200
IGEHYKAKII ASIPEGEPIT LYRQGNFIDL CRGPHSPSTG FVKHFKLMKV
210 220 230 240 250
AGAYWRGNSR NEMLQRIYGT AWATKEQLDN YLFMLEEAEK RDHRKIGKEL
260 270 280 290 300
DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYI EVKTPVLVDK
310 320 330 340 350
SLWEASGHWA KFRCDMFTLE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL
360 370 380 390 400
PLRMSEFGLC HRNEASGALH GLMRVRSLVQ DDAHIFCAEE QITDETVRFC
410 420 430 440 450
KLLTEVYKDF GFTDIKVKFS DRSEICAGND EVWDKAENAL KTAIEKAGFI
460 470 480 490 500
YTLNPGEAAF YGPKIEFVLT DAIGRQWQCG TLQMDFVLPE RLDANYIAAS
510 520 530 540 550
GEKKRPVMLH RAILGSLERF IGILIEEYAG KFPIWLAPVQ VAIATITNDL
560 570 580 590 600
NDYALEVQKT LIDNGVRTDI NISPDKINYK IREFSNQKIP IIAVIGKQEQ
610 620 630
ANKQVTIRKL GTIEQEILSI EQLIAMIKKE NSNYL
Length:635
Mass (Da):72,677
Last modified:July 1, 1997 - v1
Checksum:iB000D1514AF729D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11780 Genomic DNA. Translation: CAA72469.1.
AJ235270 Genomic DNA. Translation: CAA14684.1.
PIRiE71733.
RefSeqiNP_220607.1. NC_000963.1.
WP_004598574.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14684; CAA14684; CAA14684.
GeneIDi883948.
KEGGirpr:RP221.
PATRICifig|272947.5.peg.229.

Similar proteinsi

Entry informationi

Entry nameiSYT_RICPR
AccessioniPrimary (citable) accession number: O05947
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 22, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families