Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O05947

- SYT_RICPR

UniProt

O05947 - SYT_RICPR

Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi333 – 3331Zinc; catalyticBy similarity
    Metal bindingi384 – 3841Zinc; catalyticBy similarity
    Metal bindingi510 – 5101Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. threonine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine--tRNA ligase (EC:6.1.1.3)
    Alternative name(s):
    Threonyl-tRNA synthetase
    Short name:
    ThrRS
    Gene namesi
    Name:thrS
    Ordered Locus Names:RP221
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 635635Threonine--tRNA ligasePRO_0000101038Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi272947.RP221.

    Structurei

    3D structure databases

    ProteinModelPortaliO05947.
    SMRiO05947. Positions 241-631.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni242 – 533292CatalyticAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0441.
    HOGENOMiHOG000003880.
    KOiK01868.
    OMAiHFKLQKI.
    OrthoDBiEOG61KBFJ.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    3.40.50.800. 1 hit.
    HAMAPiMF_00184. Thr_tRNA_synth.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR012675. Beta-grasp_dom.
    IPR004095. TGS.
    IPR012676. TGS-like.
    IPR002320. Thr-tRNA-ligase_IIa.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF02824. TGS. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR01047. TRNASYNTHTHR.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55186. SSF55186. 1 hit.
    SSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00418. thrS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O05947-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINISFPDGS VKQFAQNITA FEIVNAISMS LAKAAIVVEI NGELKDLSTV    50
    IENDCKLRIL TAQDYECLEI IRHDAAHLTA EAVKELFPET QVTIGPAIEN 100
    GYYYDFARDK PFSIDDLANI EAKMQELSKK NEKITRELWD RDKAVEFFLS 150
    IGEHYKAKII ASIPEGEPIT LYRQGNFIDL CRGPHSPSTG FVKHFKLMKV 200
    AGAYWRGNSR NEMLQRIYGT AWATKEQLDN YLFMLEEAEK RDHRKIGKEL 250
    DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYI EVKTPVLVDK 300
    SLWEASGHWA KFRCDMFTLE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL 350
    PLRMSEFGLC HRNEASGALH GLMRVRSLVQ DDAHIFCAEE QITDETVRFC 400
    KLLTEVYKDF GFTDIKVKFS DRSEICAGND EVWDKAENAL KTAIEKAGFI 450
    YTLNPGEAAF YGPKIEFVLT DAIGRQWQCG TLQMDFVLPE RLDANYIAAS 500
    GEKKRPVMLH RAILGSLERF IGILIEEYAG KFPIWLAPVQ VAIATITNDL 550
    NDYALEVQKT LIDNGVRTDI NISPDKINYK IREFSNQKIP IIAVIGKQEQ 600
    ANKQVTIRKL GTIEQEILSI EQLIAMIKKE NSNYL 635
    Length:635
    Mass (Da):72,677
    Last modified:July 1, 1997 - v1
    Checksum:iB000D1514AF729D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11780 Genomic DNA. Translation: CAA72469.1.
    AJ235270 Genomic DNA. Translation: CAA14684.1.
    PIRiE71733.
    RefSeqiNP_220607.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA14684; CAA14684; CAA14684.
    GeneIDi883948.
    KEGGirpr:RP221.
    PATRICi17901202. VBIRicPro72556_0229.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11780 Genomic DNA. Translation: CAA72469.1 .
    AJ235270 Genomic DNA. Translation: CAA14684.1 .
    PIRi E71733.
    RefSeqi NP_220607.1. NC_000963.1.

    3D structure databases

    ProteinModelPortali O05947.
    SMRi O05947. Positions 241-631.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272947.RP221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA14684 ; CAA14684 ; CAA14684 .
    GeneIDi 883948.
    KEGGi rpr:RP221.
    PATRICi 17901202. VBIRicPro72556_0229.

    Phylogenomic databases

    eggNOGi COG0441.
    HOGENOMi HOG000003880.
    KOi K01868.
    OMAi HFKLQKI.
    OrthoDBi EOG61KBFJ.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    3.40.50.800. 1 hit.
    HAMAPi MF_00184. Thr_tRNA_synth.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR012675. Beta-grasp_dom.
    IPR004095. TGS.
    IPR012676. TGS-like.
    IPR002320. Thr-tRNA-ligase_IIa.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF02824. TGS. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR01047. TRNASYNTHTHR.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55186. SSF55186. 1 hit.
    SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00418. thrS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
      Andersson J.O., Andersson S.G.E.
      Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Madrid E.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.

    Entry informationi

    Entry nameiSYT_RICPR
    AccessioniPrimary (citable) accession number: O05947
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3