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O05947 (SYT_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:RP221
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00184

Subcellular location

Cytoplasm HAMAP-Rule MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 635635Threonine--tRNA ligase HAMAP-Rule MF_00184
PRO_0000101038

Regions

Region242 – 533292Catalytic HAMAP-Rule MF_00184

Sites

Metal binding3331Zinc; catalytic By similarity
Metal binding3841Zinc; catalytic By similarity
Metal binding5101Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
O05947 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: B000D1514AF729D5

FASTA63572,677
        10         20         30         40         50         60 
MINISFPDGS VKQFAQNITA FEIVNAISMS LAKAAIVVEI NGELKDLSTV IENDCKLRIL 

        70         80         90        100        110        120 
TAQDYECLEI IRHDAAHLTA EAVKELFPET QVTIGPAIEN GYYYDFARDK PFSIDDLANI 

       130        140        150        160        170        180 
EAKMQELSKK NEKITRELWD RDKAVEFFLS IGEHYKAKII ASIPEGEPIT LYRQGNFIDL 

       190        200        210        220        230        240 
CRGPHSPSTG FVKHFKLMKV AGAYWRGNSR NEMLQRIYGT AWATKEQLDN YLFMLEEAEK 

       250        260        270        280        290        300 
RDHRKIGKEL DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYI EVKTPVLVDK 

       310        320        330        340        350        360 
SLWEASGHWA KFRCDMFTLE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL PLRMSEFGLC 

       370        380        390        400        410        420 
HRNEASGALH GLMRVRSLVQ DDAHIFCAEE QITDETVRFC KLLTEVYKDF GFTDIKVKFS 

       430        440        450        460        470        480 
DRSEICAGND EVWDKAENAL KTAIEKAGFI YTLNPGEAAF YGPKIEFVLT DAIGRQWQCG 

       490        500        510        520        530        540 
TLQMDFVLPE RLDANYIAAS GEKKRPVMLH RAILGSLERF IGILIEEYAG KFPIWLAPVQ 

       550        560        570        580        590        600 
VAIATITNDL NDYALEVQKT LIDNGVRTDI NISPDKINYK IREFSNQKIP IIAVIGKQEQ 

       610        620        630 
ANKQVTIRKL GTIEQEILSI EQLIAMIKKE NSNYL 

« Hide

References

« Hide 'large scale' references
[1]"Genomic rearrangements during evolution of the obligate intracellular parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence."
Andersson J.O., Andersson S.G.E.
Microbiology 143:2783-2795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Madrid E.
[2]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y11780 Genomic DNA. Translation: CAA72469.1.
AJ235270 Genomic DNA. Translation: CAA14684.1.
PIRE71733.
RefSeqNP_220607.1. NC_000963.1.

3D structure databases

ProteinModelPortalO05947.
SMRO05947. Positions 241-631.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP221.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA14684; CAA14684; CAA14684.
GeneID883948.
KEGGrpr:RP221.
PATRIC17901202. VBIRicPro72556_0229.

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000003880.
KOK01868.
OMACVGHIKV.
OrthoDBEOG61KBFJ.
ProtClustDBPRK00413.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPMF_00184. Thr_tRNA_synth.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYT_RICPR
AccessionPrimary (citable) accession number: O05947
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries