Reviewed, UniProtKB/Swiss-Prot O05820 (MBTG_MYCTU)

Names and origin

Protein names

Recommended name:
    L-lysine 6-monooxygenase mbtG
    EC=1.14.13.59
Alternative name(s):
    Lysine 6-N-hydroxylase
    Lysine N(6)-hydroxylase
    Lysine-N-oxygenase
    Mycobactin synthetase protein G

Gene names

Name:	mbtG
Ordered Locus Names:	Rv2378c, MT2446

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro. Ref.3 Ref.4
Catalytic activity	L-lysine + NADPH + O2 = N(6)-hydroxy-L-lysine + NADP+ + H2O.
Cofactor	FAD. Ref.4
Pathway	Siderophore biosynthesis; mycobactin biosynthesis.
Induction	Induced by iron starvation conditions and during infection of human THP-1 macrophages. Transcriptionally repressed by ideR and iron By similarity.
Sequence similarities	Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Domain	Signal
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-lysine 6-monooxygenase (NADPH) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 431	410	L-lysine 6-monooxygenase mbtG		PRO_0000261312

Sequences

	Sequence		Length	Mass (Da)
	O05820-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 8D200693CB6D4EB0	FASTA	431	46,944
	10 20 30 40 50 60 MNPTLAVLGA GAKAVAVAAK ASVLRDMGVD VPDVIAVERI GVGANWQASG GWTDGAHRLG 70 80 90 100 110 120 TSPEKDVGFP YRSALVPRRN AELDERMTRY SWQSYLIATA SFAEWIDRGR PAPTHRRWSQ 130 140 150 160 170 180 YLAWVADHIG LKVIHGEVER LAVTGDRWAL CTHETTVQAD ALMITGPGQA EKSLLPGNPR 190 200 210 220 230 240 VLSIAQFWDR AAGHDRINAE RVAVIGGGET AASMLNELFR HRVSTITVIS PQVTLFTRGE 250 260 270 280 290 300 GFFENSLFSD PTDWAALTFD ERRDALARTD RGVFSATVQE ALLADDRIHH LRGRVAHAVG 310 320 330 340 350 360 RQGQIRLTLS TNRGSENFET VHGFDLVIDG SGADPLWFTS LFSQHTLDLL ELGLGGPLTA 370 380 390 400 410 420 DRLQEAIGYD LAVTDVTPKL FLPTLSGLTQ GPGFPNLSCL GLLSDRVLGA GIFTPTKHND 430 TRRSGEHQSF R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin." Quadri L.E.N., Sello J., Keating T.A., Weinreb P.H., Walsh C.T. Chem. Biol. 5:631-645(1998) [PubMed: 9831524] [Abstract] Cited for: ROLE IN MYCOBACTIN BIOSYNTHESIS. Strain: CDC 1551 / Oshkosh.
[4]	"A genetic locus required for iron acquisition in Mycobacterium tuberculosis." Krithika R., Marathe U., Saxena P., Ansari M.Z., Mohanty D., Gokhale R.S. Proc. Natl. Acad. Sci. U.S.A. 103:2069-2074(2006) [PubMed: 16461464] [Abstract] Cited for: FUNCTION, COFACTOR. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842579 Genomic DNA. Translation: CAB08475.1. AE000516 Genomic DNA. Translation: AAK46741.1.
PIR	A70588.
RefSeq	NP_216894.1. NP_336927.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	885648. 925901.
GenomeReviews	Gene locus MT2446 in contig AE000516_GR.
KEGG	mtc:MT2446. mtu:Rv2378c.
TIGR	MT2446.
Organism-specific databases
TubercuList	Rv2378c.
Phylogenomic databases
HOGENOM	HBG432039.
OMA	SIAQFWH.
Enzyme and pathway databases
BRENDA	1.14.13.59. 809.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MBTG_MYCTU

Accession

Primary (citable) accession number: O05820
Secondary accession number(s): Q7D793

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)