O05820 (MBTG_MYCTU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 66.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: L-lysine 6-monooxygenase mbtG EC=1.14.13.59 Alternative name(s): Lysine 6-N-hydroxylase Lysine N(6)-hydroxylase Lysine-N-oxygenase Mycobactin synthase protein G | ||||
| Gene names |
| ||||
| Organism | Mycobacterium tuberculosis | ||||
| Taxonomic identifier | 1773 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex |
Protein attributes
| Sequence length | 431 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro. Ref.3 Ref.4 |
| Catalytic activity | L-lysine + NADPH + O2 = N(6)-hydroxy-L-lysine + NADP+ + H2O. |
| Cofactor | FAD. Ref.4 |
| Pathway | |
| Induction | Induced by iron starvation conditions and during infection of human THP-1 macrophages. Transcriptionally repressed by ideR and iron By similarity. |
| Sequence similarities | Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Ligand | FAD Flavoprotein NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | growth Inferred from mutant phenotype. Source: MTBBASE siderophore biosynthetic process from catecholInferred from direct assay Ref.4. Source: MTBBASE |
| Molecular function | L-lysine 6-monooxygenase (NADPH) activity Inferred from direct assay Ref.4. Source: MTBBASE |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
| ||||||||||||||||||
References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX842579 Genomic DNA. Translation: CAB08475.1. AE000516 Genomic DNA. Translation: AAK46741.1. |
| PIR | A70588. |
| RefSeq | NP_216894.1. NC_000962.2. NP_336927.1. NC_002755.2. |
3D structure databases | |
| ProteinModelPortal | O05820. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBMYCT00000003018; EBMYCP00000003018; EBMYCG00000003016. EBMYCT00000071194; EBMYCP00000069253; EBMYCG00000071189. |
| GeneID | 885648. 925901. |
| GenomeReviews | Gene locus MT2446 in contig AE000516_GR. Gene locus Rv2378c in contig AL123456_GR. |
| KEGG | mtc:MT2446. mtu:Rv2378c. |
| PATRIC | 18127132. VBIMycTub22151_2674. |
| TIGR | MT2446. |
Organism-specific databases | |
| TubercuList | Rv2378c. |
Phylogenomic databases | |
| GeneTree | EBGT00050000017267. |
| HOGENOM | HBG432039. |
| OMA | IAQFWHR. |
| ProtClustDB | CLSK791811. |
Family and domain databases | |
| KO | K04793. |
| ProtoNet | Search... |
Entry information
| Entry name | MBTG_MYCTU | ||||||||
| Accession | Primary (citable) accession number: O05820 Secondary accession number(s): Q7D793 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with