Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O05724 (IPYR_BACST)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase
Gene names
Name: ppa
Synonyms: pMK2PPA
OrganismBacillus stearothermophilus (Geobacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Hide | Top

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subcellular location

Cytoplasm. HAMAP MF_00209

Sequence similarities

Belongs to the PPase family.

Hide | Top

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological processphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 165164Inorganic pyrophosphatase HAMAP MF_00209
PRO_0000137479

Sites

Metal binding571Magnesium 1 By similarity
Metal binding621Magnesium 1 By similarity
Metal binding621Magnesium 2 By similarity
Metal binding941Magnesium 1 By similarity

Experimental info

Mutagenesis471Y → F: Loss of activity. Ref.1
Mutagenesis1311Y → F: Loss of activity. Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
O05724-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3E117DFA4FA2286B

FASTA16518,927
        10         20         30         40         50         60 
MAFENKIVEA FIEIPTGSQN KYEFDKERGI FKLDRVLYSP MFYPAEYGYL QNTLALDGDP 

        70         80         90        100        110        120 
LDILVITTNP TFPGCVIDTR VIGYLNMVDS GEEDAKLIGV PVEDPRFDEV RSIEDLPQHK 

       130        140        150        160 
LKEIAHFFER YKDLQGKRTE IGTWEGPEAA AKLIDECIAR YNEQK

« Hide

Hide | Top

References

[1]"Primary structure, expression, and site-directed mutagenesis of inorganic pyrophosphatase from Bacillus stearothermophilus."
Satoh T., Shinoda H., Ishii K., Koyama M., Sakurai N., Kaji H., Hachimori A., Irie M., Samejima T.
J. Biochem. 125:48-57(1999) [PubMed: 9880796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF TYR-47 AND TYR-131.
Strain: ATCC 12016.

Hide | Top

Cross-references

Sequence databases

AB003087 Genomic DNA. Translation: BAA19837.1.
PIRJH0271.

3D structure databases

HSSPHSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBaseSearch...

Enzyme and pathway databases

BRENDA3.6.1.1. 266715.

Family and domain databases

HAMAPMF_00209.
[Tree]
InterProIPR008162. Pyrophosphatase.
[Graphical view]
Gene3DG3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHERPTHR10286. Pyrophosphatase. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
ProDomPD002014. Inorg_pphsph. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameIPYR_BACST
AccessionPrimary (citable) accession number: O05724
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents