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O05689

- CRP_PASMU

UniProt

O05689 - CRP_PASMU

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Protein
cAMP-activated global transcriptional regulator CRP
Gene
crp, PM1157
Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei96 – 961Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Sitei101 – 1011Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi56 – 627cAMP 1 By similarity
Nucleotide bindingi71 – 733cAMP 1 By similarity
Nucleotide bindingi82 – 832cAMP 1 By similarity
Nucleotide bindingi127 – 1282cAMP 1 By similarity
Nucleotide bindingi135 – 1362cAMP 2 By similarity
Nucleotide bindingi170 – 18011cAMP 2 By similarity
Add
BLAST
DNA bindingi179 – 1857H-T-H motif By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. cAMP binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1207-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
Catabolite gene activator
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Ordered Locus Names:PM1157
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209cAMP-activated global transcriptional regulator CRP
PRO_0000100149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei100 – 1001N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO05689.

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) By similarity.

Protein-protein interaction databases

STRINGi272843.PM1157.

Structurei

3D structure databases

ProteinModelPortaliO05689.
SMRiO05689. Positions 8-209.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini137 – 20973HTH crp-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 213Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Regioni52 – 587Activating region 3 (AR3); probably contacts sigma-70 (RpoD) By similarity
Regioni153 – 16210Activating region 1 (AR1); probably contacts the C-terminus of RpoA By similarity

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
KOiK10914.
OMAiSETHPEF.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05689-1 [UniParc]FASTAAdd to Basket

« Hide

MQTTPSIDPT LEWFLSHCHI HKYPSKSTLI HAGEKAETLY YLIKGSVAVL    50
VKDEDGKEMI LTYLSQGDFF GEAGLFEEGQ LRSAWIKAKS PCEIAEISYK 100
KFRQLIQVNP DILMHLSAQL ARRLQNTSRQ VSNLAFLDVT GRIAQTLLNL 150
AKMPEAMTHP DGMQIKITRQ EIGQMVGCSR ETVGRILKML EDQHLISAHG 200
KTIVVYGTR 209
Length:209
Mass (Da):23,541
Last modified:July 1, 1997 - v1
Checksum:i2E6A60E75F9CEF07
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U95380 Genomic DNA. Translation: AAB53064.1.
AE004439 Genomic DNA. Translation: AAK03241.1.
RefSeqiNP_246094.1. NC_002663.1.
WP_010907050.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03241; AAK03241; PM1157.
GeneIDi1244504.
KEGGipmu:PM1157.
PATRICi22871567. VBIPasMul88067_1168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U95380 Genomic DNA. Translation: AAB53064.1 .
AE004439 Genomic DNA. Translation: AAK03241.1 .
RefSeqi NP_246094.1. NC_002663.1.
WP_010907050.1. NC_002663.1.

3D structure databases

ProteinModelPortali O05689.
SMRi O05689. Positions 8-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM1157.

Proteomic databases

PRIDEi O05689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK03241 ; AAK03241 ; PM1157 .
GeneIDi 1244504.
KEGGi pmu:PM1157.
PATRICi 22871567. VBIPasMul88067_1168.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000250565.
KOi K10914.
OMAi SETHPEF.
OrthoDBi EOG69GZGV.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-1207-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of crp mutants in Pasteurella multocida."
    Roland K.L., Mukkur T.K., Ball T.K.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiCRP_PASMU
AccessioniPrimary (citable) accession number: O05689
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi