Reviewed, UniProtKB/Swiss-Prot O05651 (PORA_THEMA)

Names and origin

Protein names

Recommended name:
    Pyruvate synthase subunit porA
    EC=1.2.7.1
Alternative name(s):
    Pyruvate oxidoreductase alpha chain
      Short name=POR
    Pyruvic-ferredoxin oxidoreductase subunit alpha

Gene names

Name:	porA
Ordered Locus Names:	TM_0017

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro pyruvate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Pyruvate synthase subunit porA		PRO_0000099902
Regions
	Compositional bias	72 – 79	8	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	O05651-1 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: ACEF0E34C9EC1818	FASTA	392	44,112
	10 20 30 40 50 60 MERVVERVAV TGAEAVANAM RQIEPDVVAA YPITPQTPIV EYFARFVADG VVRTEMIPVE 70 80 90 100 110 120 SEHSAMSAVV GAAAAGARAM TATSANGLAL MHEIVYIAAS YRLPIVMPVV NRALSGPINI 130 140 150 160 170 180 HCDHSDAMAE RDSGWIQLFA ETNQEAYDFT ILAVRLAEHE DVRLPVMVNL DGFILSHGVE 190 200 210 220 230 240 PVEFYPDELV KKFVGELKPM YPLLDTEHPV TWGPLDLYDY YFEHKRQQIE AMENVKKVFP 250 260 270 280 290 300 EIAKEFEETF GRKYWFVEPY RMEDAEHVMV ALGSTNSTIK YVVDELREEG YKVGSLKIWM 310 320 330 340 350 360 FRPFPKEQLQ ELLNGRKSVV VLDRAVSFGA EAPLYEAVKS ALYEVAARPM LGSYVYGLGG 370 380 390 RDIKPEHIRK AFEDAINGNL IADEQRYLGL RE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima." Kletzin A., Adams M.W.W. J. Bacteriol. 178:248-257(1996) [PubMed: 8550425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-43. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima." Blamey J.M., Adams M.W.W. Biochemistry 33:1000-1007(1994) [PubMed: 8305426] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-43, CHARACTERIZATION. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85171 Genomic DNA. Translation: CAA59457.1. Different initiation. AE000512 Genomic DNA. Translation: AAD35111.1. Different initiation.
PIR	C72427. D59427.
RefSeq	NP_227833.1.
3D structure databases
HSSP	HSSP built from PDB template 1KEK based on UniProtKB P94692.
ModBase	Search...
Genome annotation databases
GeneID	896831.
GenomeReviews	Gene locus TM_0017 in contig AE000512_GR.
KEGG	tma:TM0017.
NMPDR	fig|243274.1.peg.17.
TIGR	TM_0017.
Phylogenomic databases
HOGENOM	O05651.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-423. TMAR243274:TM_0017-MON.
BRENDA	1.2.7.1. 16699.
Family and domain databases
InterPro	IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR015941. Transketolase_C-like. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF01855. POR_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PORA_THEMA

Accession

Primary (citable) accession number: O05651

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 20, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)