O05650 (PORC_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate synthase subunit porC
EC=1.2.7.1

Alternative name(s):
Pyruvate oxidoreductase gamma chain
Short name=POR
Pyruvic-ferredoxin oxidoreductase subunit gamma

Gene names

Name:	porC
Synonyms:	porG
Ordered Locus Names:	TM_0015

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Sequence caution	The sequence AAD35109.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA59455.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	pyruvate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 192

191

Pyruvate synthase subunit porC

PRO_0000099917

Experimental info

Sequence conflict

23

1

A → V in CAA59455. Ref.1

Secondary structure

1

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192

Helix Strand Turn

Details...

Beta strand

7 – 15

9

Helix

20 – 33

14

Beta strand

38 – 43

6

Beta strand

52 – 60

9

Beta strand

74 – 80

7

Helix

81 – 83

3

Helix

86 – 89

4

Beta strand

96 – 101

6

Helix

106 – 113

8

Beta strand

117 – 122

6

Helix

124 – 131

8

Beta strand

132 – 134

3

Helix

138 – 149

12

Helix

154 – 164

11

Helix

171 – 187

17

Sequences

	Sequence		Length	Mass (Da)
	O05650 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: C58296B289254B70	FASTA	192	21,270
	10 20 30 40 50 60 MPVAKKYFEI RWHGRAGQGA KSASQMLAEA ALEAGKYVQA FPEYGAERTG APMRAFNRIG 70 80 90 100 110 120 DEYIRVRSAV ENPDVVVVID ETLLSPAIVE GLSEDGILLV NTVKDFEFVR KKTGFNGKIC 130 140 150 160 170 180 VVDATDIALQ EIKRGIPNTP MLGALVRVTG IVPLEAIEKR IEKMFGKKFP QEVIDANKRA 190 LRRGYEEVKC SE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima." Kletzin A., Adams M.W.W. J. Bacteriol. 178:248-257(1996) [PubMed: 8550425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X85171 Genomic DNA. Translation: CAA59455.1. Different initiation.
AE000512 Genomic DNA. Translation: AAD35109.1. Different initiation.

PIR

A72427. C59427.

RefSeq

NP_227831.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RAA	X-ray	2.12	A	1-192	[»]

ProteinModelPortal

O05650.

SMR

O05650. Positions 5-192.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

896829.

GenomeReviews

Gene locus TM_0015 in contig AE000512_GR.

KEGG

tma:TM0015.

NMPDR

fig|243274.1.peg.15.

PATRIC

23934870. VBITheMar51294_0013.

TIGR

TM_0015.

Phylogenomic databases

HOGENOM

HBG553299.

OMA

DYVIVQD.

PhylomeDB

O05650.

ProtClustDB

CLSK874924.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-425.
TMAR243274:TM_0015-MONOMER.

Family and domain databases

InterPro

IPR011894. PorC_KorC.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002869. Pyrv_flavodox_OxRed_cen.
[Graphical view]

Gene3D

G3DSA:3.40.920.10. Pyrv_Fd/Flavodoxin_OxRdtase. 1 hit.

KO

K00172.

Pfam

PF01558. POR. 1 hit.
[Graphical view]

SUPFAM

SSF53323. Pyrv_Fd/Flavodoxin_OxRdtase. 1 hit.

TIGRFAMs

TIGR02175. PorC_KorC. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PORC_THEMA

Accession

Primary (citable) accession number: O05650

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program