O05617 (VANB_PSEUH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vanillate O-demethylase oxidoreductase
EC=1.14.13.-

Alternative name(s):
Vanillate degradation ferredoxin-like protein

Gene names

Name:

vanB

Organism

Pseudomonas sp. (strain HR199 / DSM 7063)

Taxonomic identifier

86003 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	FMN By similarity.
Pathway	Xenobiotic degradation; vanillyl-alcohol degradation.
Subunit structure	This demethylase system consists of two proteins: an oxygenase and an oxygenase reductase.
Sequence similarities	Belongs to the PDR/VanB family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Lignin degradation Transport
Ligand	2Fe-2S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Vanillate O-demethylase oxidoreductase		PRO_0000189403
Regions
	Domain	1 – 101	101	FAD-binding FR-type
	Domain	232 – 317	86	2Fe-2S ferredoxin-type
	Nucleotide binding	105 – 220	116	NAD
Sites
	Metal binding	266	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	271	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	274	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	304	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05617 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 42C8D6024FA41B64	FASTA	317	34,461
	10 20 30 40 50 60 MIEVIISAMR LVAQDIISLE FVRADGGLLP PVEAGAHVDV HLPGGLIRQY SLWNQPGAQS 70 80 90 100 110 120 HYCIGVLKDP ASRGGSKAVH ENLRVGMRVQ ISEPRNLFPL EEGVERSLLF AGGIGITPIL 130 140 150 160 170 180 CMAQELAARE QDFELHYCAR STDRAAFVEW LKVCDFADHV RFHFDNGPDQ QKLNAAALLA 190 200 210 220 230 240 AEAEGTHLYV CGPGGFMGHV LDTAKEQGWA DNRLHREYFA AAPNVSADDG SFEVRIHSTG 250 260 270 280 290 300 QVLQVPADQT VSQVLDAAGI IVPVSCEQGI CGTCITRVVD GEPDHRDFFL TDAEKAKNDQ 310 FTPCCSRAKS ACLVLDL

References

[1]	"Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate." Priefert H., Rabenhorst J., Steinbuechel A. J. Bacteriol. 179:2595-2607(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11521 Genomic DNA. Translation: CAA72288.1.
3D structure databases
ProteinModelPortal	O05617.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14063.
UniPathway	UPA00217.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR006058. 2Fe2S_fd_BS. IPR012675. Beta-grasp_dom. IPR017927. Fd_Rdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD-bd. IPR000951. Ph_dOase_redase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00409. PHDIOXRDTASE.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VANB_PSEUH

Accession

Primary (citable) accession number: O05617

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program