ID   VANA_PSEUH              Reviewed;         354 AA.
AC   O05616;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=Vanillate O-demethylase oxygenase subunit;
DE            EC=1.14.13.82;
DE   AltName: Full=4-hydroxy-3-methoxybenzoate demethylase;
GN   Name=vanA;
OS   Pseudomonas sp. (strain HR199 / DSM 7063).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=86003;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97252489; PubMed=9098058;
RA   Priefert H., Rabenhorst J., Steinbuechel A.;
RT   "Molecular characterization of genes of Pseudomonas sp. strain HR199
RT   involved in bioconversion of vanillin to protocatechuate.";
RL   J. Bacteriol. 179:2595-2607(1997).
CC   -!- CATALYTIC ACTIVITY: Vanillate + O(2) + NADH = 3,4-
CC       dihydroxybenzoate + NAD(+) + H(2)O + formaldehyde.
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster (Probable).
CC   -!- COFACTOR: Binds 1 iron ion (Probable).
CC   -!- PATHWAY: Xenobiotic degradation; vanillyl-alcohol degradation.
CC   -!- SUBUNIT: This demethylase system consists of two proteins: an
CC       oxygenase and an oxygenase reductase.
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase alpha subunit family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; Y11521; CAA72287.1; -; Genomic_DNA.
DR   BioCyc; MetaCyc:MON-14062; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0018489; F:vanillate monooxygenase activity; IEA:EC.
DR   GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Iron; Iron-sulfur;
KW   Lignin degradation; Metal-binding; Monooxygenase; NAD; Oxidoreductase.
FT   CHAIN         1    354       Vanillate O-demethylase oxygenase
FT                                subunit.
FT                                /FTId=PRO_0000085059.
FT   DOMAIN        7    107       Rieske.
FT   METAL        47     47       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        49     49       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL        66     66       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        69     69       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
SQ   SEQUENCE   354 AA;  39491 MW;  970AD00DD8A870C3 CRC64;
     MFPKNAWYVA CTPDEIADKP LGRQICNEKI VFYRGPEGRV AAVEDFCPHR GAPLSLGFVR
     DGKLICGYHG LEMGCEGKTL AMPGQRVQGF PCIKSYAVEE RYGFIWVWPG DRELADPALI
     HHLEWADNPE WAYGGGLYHI ACDYRLMIDN LMDLTHETYV HASSIGQKEI DEAPVSTRVE
     GDTVITSRYM DNVMAPPFWR AALRGNGLAD DVPVDRWQIC RFAPPSHVLI EVGVAHAGKG
     GYDAPAEYKA GSIVVDFITP ESDTSIWYFW GMARNFRPQG TELTETIRVG QGKIFAEDLD
     MLEQQQRNLL AYPERQLLKL NIDAGGVQSR RVIDRILAAE QEAADAALIA RSAS
//