Reviewed, UniProtKB/Swiss-Prot O05616 (VANA_PSEUH)

Names and origin

Protein names

Recommended name:
    Vanillate O-demethylase oxygenase subunit
    EC=1.14.13.82
Alternative name(s):
    4-hydroxy-3-methoxybenzoate demethylase

Gene names

Name:

vanA

Organism

Pseudomonas sp. (strain HR199 / DSM 7063)

Taxonomic identifier

86003 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Vanillate + O2 + NADH = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Xenobiotic degradation; vanillyl-alcohol degradation.
Subunit structure	This demethylase system consists of two proteins: an oxygenase and an oxygenase reductase.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Lignin degradation
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW vanillate monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Vanillate O-demethylase oxygenase subunit		PRO_0000085059
Regions
	Domain	7 – 107	101	Rieske
Sites
	Metal binding	47	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	49	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	66	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	69	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05616-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 970AD00DD8A870C3	FASTA	354	39,491
	10 20 30 40 50 60 MFPKNAWYVA CTPDEIADKP LGRQICNEKI VFYRGPEGRV AAVEDFCPHR GAPLSLGFVR 70 80 90 100 110 120 DGKLICGYHG LEMGCEGKTL AMPGQRVQGF PCIKSYAVEE RYGFIWVWPG DRELADPALI 130 140 150 160 170 180 HHLEWADNPE WAYGGGLYHI ACDYRLMIDN LMDLTHETYV HASSIGQKEI DEAPVSTRVE 190 200 210 220 230 240 GDTVITSRYM DNVMAPPFWR AALRGNGLAD DVPVDRWQIC RFAPPSHVLI EVGVAHAGKG 250 260 270 280 290 300 GYDAPAEYKA GSIVVDFITP ESDTSIWYFW GMARNFRPQG TELTETIRVG QGKIFAEDLD 310 320 330 340 350 MLEQQQRNLL AYPERQLLKL NIDAGGVQSR RVIDRILAAE QEAADAALIA RSAS

References

[1]	"Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate." Priefert H., Rabenhorst J., Steinbuechel A. J. Bacteriol. 179:2595-2607(1997) [PubMed: 9098058] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11521 Genomic DNA. Translation: CAA72287.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-14062.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VANA_PSEUH

Accession

Primary (citable) accession number: O05616

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)