Reviewed, UniProtKB/Swiss-Prot O05542 (DHET_GLUOX)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase [acceptor]
    EC=1.1.99.8
Alternative name(s):
    G3-ADH subunit I

Gene names

Name:	adhA
Ordered Locus Names:	GOX1068

Organism

Gluconobacter oxydans (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dehydration of primary alcohols (exception: methanol) By similarity.
Catalytic activity	A primary alcohol + acceptor = an aldehyde + reduced acceptor.
Cofactor	Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-141-Cys-142 and the indole ring of Trp-279 By similarity. Binds 1 calcium ion per subunit By similarity. Binds 1 heme group per subunit By similarity.
Subunit structure	Heterotrimer (dehydrogenase, cytochrome and protein adhS), that forms the alcohol dehydrogenase complex.
Subcellular location	Cell membrane; Peripheral membrane protein; Periplasmic side Potential.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family. Contains 1 cytochrome c domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond PQQ Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alcohol dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34
	Chain	35 – 757	723	Alcohol dehydrogenase [acceptor]		PRO_0000025562
Regions
	Domain	637 – 726	90	Cytochrome c
Sites
	Active site	342	1	Proton acceptor Potential
	Metal binding	215	1	Calcium By similarity
	Metal binding	297	1	Calcium By similarity
	Metal binding	657	1	Iron (heme axial ligand) By similarity
	Binding site	653	1	Heme (covalent) By similarity
	Binding site	656	1	Heme (covalent) By similarity
Amino acid modifications
	Modified residue	35	1	Pyrrolidone carboxylic acid
	Disulfide bond	141 ↔ 142		By similarity
Experimental info
	Sequence conflict	230	1	G → S in BAA19753. Ref.1
	Sequence conflict	675	1	A → R in BAA19753. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O05542-1 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 56B3940B711BB0CC	FASTA	757	82,853
	10 20 30 40 50 60 MTSGLLTPIK VTKKRLLSCA AALAFSAAVP VAFAQEDTGT AITSSDNGGH PGDWLSYGRS 70 80 90 100 110 120 YSEQRYSPLD QINTENVGKL KLAWHYDLDT NRGQEGTPLI VNGVMYATTN WSKMKALDAA 130 140 150 160 170 180 TGKLLWSYDP KVPGNIADRG CCDTVSRGAA YWNGKVYFGT FDGRLIALDA KTGKLVWSVY 190 200 210 220 230 240 TIPKEAQLGH QRSYTVDGAP RIAKGKVLIG NGGAEFGARG FVSAFDAETG KLDWRFFTVP 250 260 270 280 290 300 NPENKPDGAA SDDILMSKAY PTWGKNGAWK QQGGGGTVWD SLVYDPVTDL VYLGVGNGSP 310 320 330 340 350 360 WNYKFRSEGK GDNLFLGSIV AINPDTGKYV WHFQETPMDE WDYTSVQQIM TLDMPVNGEM 370 380 390 400 410 420 RHVIVHAPKN GFFYIIDAKT GKFITGKPYT YENWANGLDP VTGRPNYVPD ALWTLTGKPW 430 440 450 460 470 480 LGIPGELGGH NFAAMAYSPK TKLVYIPAQQ IPLLYDGQKG GFKAYHDAWN LGLDMNKIGL 490 500 510 520 530 540 FDDNDPEHVA AKKDFLKVLK GWTVAWDPEK MAPAFTINHK GPWNGGLLAT AGNVIFQGLA 550 560 570 580 590 600 NGEFHAYDAT NGNDLYSFPA QSAIIAPPVT YTANGKQYVA VEVGWGGIYP FLYGGVARTS 610 620 630 640 650 660 GWTVNHSRVI AFSLDGKDSL PPKNELGFTP VKPVPTYDEA RQKDGYFMYQ TFCSACHGDN 670 680 690 700 710 720 AISGGVLPDL RWSGAPRGRE SFYKLVGRGA LTAYGMDRFD TSMTPEQIED IRNFIVKRAN 730 740 750 ESYDDEVKAR ENSTGVPNDQ FLNVPQSTAD VPTADHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the genes encoding the three-component membrane-bound alcohol dehydrogenase from Gluconobacter suboxydans and their expression in Acetobacter pasteurianus." Kondo K., Horinouchi S. Appl. Environ. Microbiol. 63:1131-1138(1997) [PubMed: 9055427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-50. Strain: ATCC 621 / IFO 3172 / NCIB 7069.
[2]	"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86375 Genomic DNA. Translation: BAA19753.1. CP000009 Genomic DNA. Translation: AAW60837.1.
RefSeq	YP_191493.1.
3D structure databases
HSSP	HSSP built from PDB template 1KB0 based on UniProtKB Q46444.
ModBase	Search...
Genome annotation databases
GeneID	3250283.
GenomeReviews	Gene locus GOX1068 in contig CP000009_GR.
KEGG	gox:GOX1068.
NMPDR	fig|290633.1.peg.1038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O05542.
OMA	O05542. MYVTASW.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX1068-MON.
BRENDA	1.1.99.8. 3050.
Family and domain databases
InterPro	IPR009056. Cyt_c_monohaem. IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR017512. PQQ_MeOH/EtOH_DH. IPR002372. PQQ_repeat. IPR011047. Quino_AlcDH-like. IPR001479. Quinoprotein_DH_CS. [Graphical view]
Gene3D	G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit. G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
Pfam	PF01011. PQQ. 6 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 6 hits. [Graphical view]
TIGRFAMs	TIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITE	PS00363. BACTERIAL_PQQ_1. 1 hit. PS00364. BACTERIAL_PQQ_2. 1 hit. PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHET_GLUOX

Accession

Primary (citable) accession number: O05542
Secondary accession number(s): Q5FS09

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)