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Protein

Mannan endo-1,4-beta-mannosidase

Gene

gmuG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of glucomannan. Catalyzes the endo hydrolysis of beta-1,4-linked mannan, galactomannan and glucomannan.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.1 Publication

Kineticsi

Kcat is 330 min(-1) for mannanase activity with galactomannan as substrate. Kcat is 350 min(-1) for mannanase activity with glucomannan as substrate.1 Publication

Manual assertion based on experiment ini

  1. KM=2.7 mg/ml for glucomannan1 Publication
  2. KM=4.5 mg/ml for galactomannan1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei131SubstrateBy similarity1
    Sitei192Plays an important role in maintaining the position of the catalytic nucleophileBy similarity1
    Active sitei193Proton donorBy similarity1
    Binding sitei198SubstrateBy similarity1
    Binding sitei268SubstrateBy similarity1
    Active sitei292NucleophileBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciBSUB:BSU05880-MONOMER.
    BRENDAi3.2.1.78. 658.

    Protein family/group databases

    CAZyiGH26. Glycoside Hydrolase Family 26.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase1 Publication (EC:3.2.1.781 Publication)
    Alternative name(s):
    1,4-beta-D-mannan mannanohydrolase1 Publication
    Beta-mannanase1 Publication
    Glucomannan utilization protein G1 Publication
    Gene namesi
    Name:gmuG1 Publication
    Synonyms:ydhT1 Publication
    Ordered Locus Names:BSU05880
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 261 PublicationAdd BLAST26
    ChainiPRO_000036086627 – 362Mannan endo-1,4-beta-mannosidaseAdd BLAST336

    Proteomic databases

    PaxDbiO05512.

    Expressioni

    Inductioni

    Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100003293.

    Structurei

    Secondary structure

    1362
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi38 – 49Combined sources12
    Helixi50 – 52Combined sources3
    Beta strandi53 – 56Combined sources4
    Beta strandi61 – 66Combined sources6
    Turni67 – 70Combined sources4
    Helixi74 – 83Combined sources10
    Beta strandi88 – 96Combined sources9
    Helixi104 – 107Combined sources4
    Helixi113 – 121Combined sources9
    Beta strandi125 – 131Combined sources7
    Beta strandi137 – 139Combined sources3
    Helixi148 – 155Combined sources8
    Helixi160 – 180Combined sources21
    Turni181 – 183Combined sources3
    Beta strandi186 – 189Combined sources4
    Beta strandi196 – 199Combined sources4
    Helixi211 – 229Combined sources19
    Beta strandi236 – 241Combined sources6
    Turni250 – 253Combined sources4
    Turni257 – 259Combined sources3
    Beta strandi261 – 270Combined sources10
    Helixi272 – 274Combined sources3
    Helixi278 – 282Combined sources5
    Beta strandi288 – 297Combined sources10
    Helixi304 – 314Combined sources11
    Beta strandi318 – 323Combined sources6
    Helixi326 – 328Combined sources3
    Helixi330 – 332Combined sources3
    Helixi336 – 341Combined sources6
    Beta strandi351 – 353Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2WHKX-ray1.70A27-362[»]
    3CBWX-ray1.27A/B21-362[»]
    ProteinModelPortaliO05512.
    SMRiO05512.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO05512.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini38 – 349GH26PROSITE-ProRule annotationAdd BLAST312

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni324 – 325Substrate bindingBy similarity2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 26 family.PROSITE-ProRule annotationCurated
    Contains 1 GH26 (glycosyl hydrolase family 26) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4105D3P. Bacteria.
    COG4124. LUCA.
    HOGENOMiHOG000009206.
    InParanoidiO05512.
    KOiK01218.
    OMAiSPAIYGC.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR022790. GH26_dom.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR016714. Mannan-1_4-b-mannosidase.
    [Graphical view]
    PANTHERiPTHR40079. PTHR40079. 1 hit.
    PfamiPF02156. Glyco_hydro_26. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018168. Mannan-1_4-beta-mannosidase. 1 hit.
    PRINTSiPR00739. GLHYDRLASE26.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS51764. GH26. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O05512-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFKKHTISLL IIFLLASAVL AKPIEAHTVS PVNPNAQQTT KTVMNWLAHL
    60 70 80 90 100
    PNRTENRVLS GAFGGYSHDT FSMAEADRIR SATGQSPAIY GCDYARGWLE
    110 120 130 140 150
    TANIEDSIDV SCNGDLMSYW KNGGIPQISL HLANPAFQSG HFKTPITNDQ
    160 170 180 190 200
    YKKILDSSTV EGKRLNAMLS KIADGLQELE NQGVPVLFRP LHEMNGEWFW
    210 220 230 240 250
    WGLTSYNQKD NERISLYKQL YKKIYHYMTD TRGLDHLIWV YSPDANRDFK
    260 270 280 290 300
    TDFYPGASYV DIVGLDAYFQ DAYSINGYDQ LTALNKPFAF TEVGPQTANG
    310 320 330 340 350
    SFDYSLFINA IKQKYPKTIY FLAWNDEWSA AVNKGASALY HDSWTLNKGE
    360
    IWNGDSLTPI VE
    Length:362
    Mass (Da):40,892
    Last modified:July 7, 2009 - v2
    Checksum:iC7E1D527AA001B14
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti153K → N in BAA19712 (PubMed:9202461).Curated1
    Sequence conflicti158 – 160STV → ATA in BAA19712 (PubMed:9202461).Curated3

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D88802 Genomic DNA. Translation: BAA19712.1.
    AL009126 Genomic DNA. Translation: CAB12407.2.
    PIRiH69785.
    RefSeqiNP_388469.2. NC_000964.3.
    WP_003244107.1. NZ_JNCM01000031.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12407; CAB12407; BSU05880.
    GeneIDi938034.
    KEGGibsu:BSU05880.
    PATRICi18972792. VBIBacSub10457_0617.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D88802 Genomic DNA. Translation: BAA19712.1.
    AL009126 Genomic DNA. Translation: CAB12407.2.
    PIRiH69785.
    RefSeqiNP_388469.2. NC_000964.3.
    WP_003244107.1. NZ_JNCM01000031.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2WHKX-ray1.70A27-362[»]
    3CBWX-ray1.27A/B21-362[»]
    ProteinModelPortaliO05512.
    SMRiO05512.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100003293.

    Protein family/group databases

    CAZyiGH26. Glycoside Hydrolase Family 26.

    Proteomic databases

    PaxDbiO05512.

    Protocols and materials databases

    DNASUi938034.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB12407; CAB12407; BSU05880.
    GeneIDi938034.
    KEGGibsu:BSU05880.
    PATRICi18972792. VBIBacSub10457_0617.

    Phylogenomic databases

    eggNOGiENOG4105D3P. Bacteria.
    COG4124. LUCA.
    HOGENOMiHOG000009206.
    InParanoidiO05512.
    KOiK01218.
    OMAiSPAIYGC.

    Enzyme and pathway databases

    BioCyciBSUB:BSU05880-MONOMER.
    BRENDAi3.2.1.78. 658.

    Miscellaneous databases

    EvolutionaryTraceiO05512.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR022790. GH26_dom.
    IPR000805. Glyco_hydro_26.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR016714. Mannan-1_4-b-mannosidase.
    [Graphical view]
    PANTHERiPTHR40079. PTHR40079. 1 hit.
    PfamiPF02156. Glyco_hydro_26. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018168. Mannan-1_4-beta-mannosidase. 1 hit.
    PRINTSiPR00739. GLHYDRLASE26.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS51764. GH26. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMANB_BACSU
    AccessioniPrimary (citable) accession number: O05512
    Secondary accession number(s): Q797D6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 7, 2009
    Last modified: November 2, 2016
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.