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O05508 (GMUD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-glucosidase GmuD

EC=3.2.1.86
Alternative name(s):
Aryl-phospho-beta-D-glucosidase BglD
Glucomannan utilization protein D
Gene names
Name:gmuD
Synonyms:bglD, ydhP
Ordered Locus Names:BSU05840
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in vivo. Ref.3 Ref.4

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Developmental stage

Expressed at only a very low level in exponential-phase cells and germinating spores, but is expressed at a higher levels upon entry into the stationary phase of growth. Ref.3

Induction

Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR. Is not induced by aryl-beta-D-glucosides such as arbutin or salicin. Ref.3 Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function6-phospho-beta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4654656-phospho-beta-glucosidase GmuD
PRO_0000371418

Sites

Active site1701Proton donor Potential
Active site3681Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O05508 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 9DD069BF2151D590

FASTA46554,334
        10         20         30         40         50         60 
MAHTEQYRFP KDFWWGSSAS ATQMEGAADR DGKGQNIWDY WFEKEPHRFF DHVGPADTSQ 

        70         80         90        100        110        120 
FYDNYKEDIR LMKELGHNSF RMSISWSRLI PNGTGEINDK AADFYNNVID ELIANGIEPF 

       130        140        150        160        170        180 
VNLFHFDMPM ALQKIGGWVN RETVDAYENY ARTCFRLFGG RVKKWFTHNE PIVPVEGGYL 

       190        200        210        220        230        240 
YDFHYPNKVD FKEAVQVGFH TMLSSARAIQ AYREMKQDGK IGIILNLTPS YPRSSHPADV 

       250        260        270        280        290        300 
KAGEIADAFF NRSFLDPSVK GEFPKELVDI LKHEGFMPDY NAEDLDIIKK NTVDLLGVNY 

       310        320        330        340        350        360 
YQPRRVKAKE HLPNPDAPFL PDRYFDPYVM PGRKMNPHRG WEIYEKGVYD ILINLKENYG 

       370        380        390        400        410        420 
NIECFISENG MGVEGEERFR DEQGIIQDDY RIEFIKEHLK WIHRAIQEGS NVKGYHLWTF 

       430        440        450        460 
MDNWSWTNAY KNRYGFVSVN LEKDGERTVK KSGKWFKEVA EHSGF 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome."
Sadaie Y., Yata K., Fujita M., Sagai H., Itaya M., Kasahara Y., Ogasawara N.
Microbiology 143:1861-1866(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
Strain: 168 / PS832.
[4]"Glucomannan utilization operon of Bacillus subtilis."
Sadaie Y., Nakadate H., Fukui R., Yee L.M., Asai K.
FEMS Microbiol. Lett. 279:103-109(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY GLUCOMANNAN, FUNCTION IN GLUCOMANNAN UTILIZATION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D88802 Genomic DNA. Translation: BAA19708.1.
AL009126 Genomic DNA. Translation: CAB12403.1.
PIRD69785.
RefSeqNP_388465.1. NC_000964.3.

3D structure databases

ProteinModelPortalO05508.
SMRO05508. Positions 6-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU05840.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbO05508.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12403; CAB12403; BSU05840.
GeneID939872.
KEGGbsu:BSU05840.
PATRIC18972784. VBIBacSub10457_0613.

Organism-specific databases

GenoListBSU05840. [Micado]

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088632.
KOK01223.
OMAWIPLNEP.
OrthoDBEOG60658J.
PhylomeDBO05508.

Enzyme and pathway databases

BioCycBSUB:BSU05840-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGMUD_BACSU
AccessionPrimary (citable) accession number: O05508
Secondary accession number(s): Q797D9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList