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Protein

Beta-lactamase

Gene

ampC

Organism
Psychrobacter immobilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei188 – 1881Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Protein family/group databases

MEROPSiS12.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
Synonyms:bla
OrganismiPsychrobacter immobilis
Taxonomic identifieri498 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 39391 PublicationAdd
BLAST
Chaini40 – 401362Beta-lactamasePRO_0000016964Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO05465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni353 – 3553Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-C beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O05465-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLFTSTLTA KKSSTHKPLI SLALSVLIST LLISETAQAA DANDRLEQEV
60 70 80 90 100
DKQAKQLMAQ YQIPGMAFGI IVDGKSHFYN YGLADKQRNQ PVSEDTIFEL
110 120 130 140 150
GSVSKTFAAT LASYSELNGT LSLDDTADKY IPYLKNSAIG NTKLISLVTY
160 170 180 190 200
SAGGYHYRCL KTLENNKELL QYYKSWHPDF PVNSKRLYSN ASIGLFGYIS
210 220 230 240 250
ALSMHSDYTK LIENTVLPSL KMTNTFVDVP ANKMEDYAFG YNAAGEPIRV
260 270 280 290 300
NPGMLDAEAY GIKSTSADMT RFMAANMGLV TVDSQMQQAL DNNRKGYYRT
310 320 330 340 350
KSFTQGLAWE MYPLPTTLQQ LVEGNSTETI LQPQPIQLNE PPTPVLNDVW
360 370 380 390 400
VNKTGATNGF GAYIAYMPAK KTGMFILANK NYPNTERVKA AYTILDSVMN

N
Length:401
Mass (Da):44,451
Last modified:July 1, 1997 - v1
Checksum:i93F0DB278EA8E043
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83586 Genomic DNA. Translation: CAA58569.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83586 Genomic DNA. Translation: CAA58569.1.

3D structure databases

ProteinModelPortaliO05465.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS12.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Enzymes from cold-adapted microorganisms. The class C beta-lactamase from the antarctic psychrophile Psychrobacter immobilis A5."
    Feller G., Zekhnini Z., Lamotte-Brasseur J., Gerday C.
    Eur. J. Biochem. 244:186-191(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-47, CHARACTERIZATION.
    Strain: A5.

Entry informationi

Entry nameiAMPC_PSYIM
AccessioniPrimary (citable) accession number: O05465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.