O05465 (AMPC_PSYIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Cephalosporinase

Gene names

Name:	ampC
Synonyms:	bla

Organism

Psychrobacter immobilis

Taxonomic identifier

498 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Ref.1
	Chain	40 – 401	362	Beta-lactamase		PRO_0000016964
Regions
	Region	353 – 355	3	Substrate binding By similarity
Sites
	Active site	102	1	Acyl-ester intermediate By similarity
	Active site	188	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O05465 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 93F0DB278EA8E043	FASTA	401	44,451
	10 20 30 40 50 60 MKLFTSTLTA KKSSTHKPLI SLALSVLIST LLISETAQAA DANDRLEQEV DKQAKQLMAQ 70 80 90 100 110 120 YQIPGMAFGI IVDGKSHFYN YGLADKQRNQ PVSEDTIFEL GSVSKTFAAT LASYSELNGT 130 140 150 160 170 180 LSLDDTADKY IPYLKNSAIG NTKLISLVTY SAGGYHYRCL KTLENNKELL QYYKSWHPDF 190 200 210 220 230 240 PVNSKRLYSN ASIGLFGYIS ALSMHSDYTK LIENTVLPSL KMTNTFVDVP ANKMEDYAFG 250 260 270 280 290 300 YNAAGEPIRV NPGMLDAEAY GIKSTSADMT RFMAANMGLV TVDSQMQQAL DNNRKGYYRT 310 320 330 340 350 360 KSFTQGLAWE MYPLPTTLQQ LVEGNSTETI LQPQPIQLNE PPTPVLNDVW VNKTGATNGF 370 380 390 400 GAYIAYMPAK KTGMFILANK NYPNTERVKA AYTILDSVMN N

References

[1]

"Enzymes from cold-adapted microorganisms. The class C beta-lactamase from the antarctic psychrophile Psychrobacter immobilis A5." Feller G., Zekhnini Z., Lamotte-Brasseur J., Gerday C. Eur. J. Biochem. 244:186-191(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-47, CHARACTERIZATION. Strain: A5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83586 Genomic DNA. Translation: CAA58569.1.
3D structure databases
ProteinModelPortal	O05465.
ModBase	Search...
Protein family/group databases
MEROPS	S12.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR001586. Beta-lactam_class-C_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00336. BETA_LACTAMASE_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPC_PSYIM

Accession

Primary (citable) accession number: O05465

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program