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O05465 (AMPC_PSYIM) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Cephalosporinase
Gene names
Name:ampC
Synonyms:bla
OrganismPsychrobacter immobilis
Taxonomic identifier498 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Secreted.

Sequence similarities

Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

outer membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Ref.1
Chain40 – 401362Beta-lactamase
PRO_0000016964

Regions

Region353 – 3553Substrate binding By similarity

Sites

Active site1021Acyl-ester intermediate By similarity
Active site1881Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
O05465 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 93F0DB278EA8E043

FASTA40144,451
        10         20         30         40         50         60 
MKLFTSTLTA KKSSTHKPLI SLALSVLIST LLISETAQAA DANDRLEQEV DKQAKQLMAQ 

        70         80         90        100        110        120 
YQIPGMAFGI IVDGKSHFYN YGLADKQRNQ PVSEDTIFEL GSVSKTFAAT LASYSELNGT 

       130        140        150        160        170        180 
LSLDDTADKY IPYLKNSAIG NTKLISLVTY SAGGYHYRCL KTLENNKELL QYYKSWHPDF 

       190        200        210        220        230        240 
PVNSKRLYSN ASIGLFGYIS ALSMHSDYTK LIENTVLPSL KMTNTFVDVP ANKMEDYAFG 

       250        260        270        280        290        300 
YNAAGEPIRV NPGMLDAEAY GIKSTSADMT RFMAANMGLV TVDSQMQQAL DNNRKGYYRT 

       310        320        330        340        350        360 
KSFTQGLAWE MYPLPTTLQQ LVEGNSTETI LQPQPIQLNE PPTPVLNDVW VNKTGATNGF 

       370        380        390        400 
GAYIAYMPAK KTGMFILANK NYPNTERVKA AYTILDSVMN N 

« Hide

References

[1]"Enzymes from cold-adapted microorganisms. The class C beta-lactamase from the antarctic psychrophile Psychrobacter immobilis A5."
Feller G., Zekhnini Z., Lamotte-Brasseur J., Gerday C.
Eur. J. Biochem. 244:186-191(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-47, CHARACTERIZATION.
Strain: A5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83586 Genomic DNA. Translation: CAA58569.1.

3D structure databases

ProteinModelPortalO05465.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS12.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPC_PSYIM
AccessionPrimary (citable) accession number: O05465
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: October 16, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families