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Protein

Cystathionine gamma-lyase

Gene

mccB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide.1 Publication

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.1 Publication
L-homocysteine + H2O = H2S + NH3 + 2-oxobutanoate.1 Publication

Cofactori

Kineticsi

  1. KM=3 mM for cystathionine1 Publication
  1. Vmax=2 µmol/min/mg enzyme for cystathionine gamma-lyase reaction1 Publication

GO - Molecular functioni

GO - Biological processi

  • homocysteine catabolic process Source: CAFA
  • positive regulation of cell proliferation Source: CAFA

Keywordsi

Molecular functionLyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciBSUB:BSU27250-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Gamma-cystathionase
Homocysteine gamma-lyase (EC:4.4.1.2)
Gene namesi
Name:mccB
Synonyms:yrhB
Ordered Locus Names:BSU27250
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003606521 – 379Cystathionine gamma-lyaseAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiO05394
PRIDEiO05394

Expressioni

Inductioni

By methionine. Repressed by sulfate and cysteine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014891

Structurei

3D structure databases

ProteinModelPortaliO05394
SMRiO05394
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28 Bacteria
COG0626 LUCA
HOGENOMiHOG000246415
InParanoidiO05394
KOiK17217
OMAiMQTKLIH
PhylomeDBiO05394

Family and domain databases

CDDicd00614 CGS_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11808 PTHR11808, 1 hit
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
PIRSFiPIRSF001434 CGS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

O05394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKTLMIHG GITGDEKTGA VSVPIYQVST YKQPKAGQHT GYEYSRTANP
60 70 80 90 100
TRTALEALVT ELESGEAGYA FSSGMAAITA VMMLFNSGDH VVLTDDVYGG
110 120 130 140 150
TYRVMTKVLN RLGIESTFVD TSSREEVEKA IRPNTKAIYI ETPTNPLLKI
160 170 180 190 200
TDLTLMADIA KKAGVLLIVD NTFNTPYFQQ PLTLGADIVL HSATKYLGGH
210 220 230 240 250
SDVVGGLVVT ASKELGEELH FVQNSTGGVL GPQDSWLLMR GIKTLGLRME
260 270 280 290 300
AIDQNARKIA SFLENHPAVQ TLYYPGSSNH PGHELAKTQG AGFGGMISFD
310 320 330 340 350
IGSEERVDAF LGNLKLFTIA ESLGAVESLI SVPARMTHAS IPRERRLELG
360 370
ITDGLIRISV GIEDAEDLLE DIGQALENI
Length:379
Mass (Da):40,886
Last modified:July 1, 1997 - v1
Checksum:i44DD71446F6E15B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93874 Genomic DNA Translation: AAB80859.1
AL009126 Genomic DNA Translation: CAB14667.1
PIRiA69974
RefSeqiNP_390603.1, NC_000964.3
WP_003229810.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14667; CAB14667; BSU27250
GeneIDi936947
KEGGibsu:BSU27250
PATRICifig|224308.179.peg.2961

Entry informationi

Entry nameiMCCB_BACSU
AccessioniPrimary (citable) accession number: O05394
Secondary accession number(s): Q795Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health