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Protein

Cystathionine gamma-lyase

Gene

mccB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide.1 Publication

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.1 Publication
L-homocysteine + H2O = H2S + NH3 + 2-oxobutanoate.1 Publication

Cofactori

Kineticsi

  1. KM=3 mM for cystathionine1 Publication
  1. Vmax=2 µmol/min/mg enzyme for cystathionine gamma-lyase reaction1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciBSUB:BSU27250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Gamma-cystathionase
Homocysteine gamma-lyase (EC:4.4.1.2)
Gene namesi
Name:mccB
Synonyms:yrhB
Ordered Locus Names:BSU27250
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003606521 – 379Cystathionine gamma-lyaseAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiO05394.

Expressioni

Inductioni

By methionine. Repressed by sulfate and cysteine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014891.

Structurei

3D structure databases

ProteinModelPortaliO05394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
HOGENOMiHOG000246415.
InParanoidiO05394.
KOiK17217.
OMAiFHQNAMG.
PhylomeDBiO05394.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKTLMIHG GITGDEKTGA VSVPIYQVST YKQPKAGQHT GYEYSRTANP
60 70 80 90 100
TRTALEALVT ELESGEAGYA FSSGMAAITA VMMLFNSGDH VVLTDDVYGG
110 120 130 140 150
TYRVMTKVLN RLGIESTFVD TSSREEVEKA IRPNTKAIYI ETPTNPLLKI
160 170 180 190 200
TDLTLMADIA KKAGVLLIVD NTFNTPYFQQ PLTLGADIVL HSATKYLGGH
210 220 230 240 250
SDVVGGLVVT ASKELGEELH FVQNSTGGVL GPQDSWLLMR GIKTLGLRME
260 270 280 290 300
AIDQNARKIA SFLENHPAVQ TLYYPGSSNH PGHELAKTQG AGFGGMISFD
310 320 330 340 350
IGSEERVDAF LGNLKLFTIA ESLGAVESLI SVPARMTHAS IPRERRLELG
360 370
ITDGLIRISV GIEDAEDLLE DIGQALENI
Length:379
Mass (Da):40,886
Last modified:July 1, 1997 - v1
Checksum:i44DD71446F6E15B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93874 Genomic DNA. Translation: AAB80859.1.
AL009126 Genomic DNA. Translation: CAB14667.1.
PIRiA69974.
RefSeqiNP_390603.1. NC_000964.3.
WP_003229810.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14667; CAB14667; BSU27250.
GeneIDi936947.
KEGGibsu:BSU27250.
PATRICi18977306. VBIBacSub10457_2842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93874 Genomic DNA. Translation: AAB80859.1.
AL009126 Genomic DNA. Translation: CAB14667.1.
PIRiA69974.
RefSeqiNP_390603.1. NC_000964.3.
WP_003229810.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO05394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014891.

Proteomic databases

PaxDbiO05394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14667; CAB14667; BSU27250.
GeneIDi936947.
KEGGibsu:BSU27250.
PATRICi18977306. VBIBacSub10457_2842.

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
HOGENOMiHOG000246415.
InParanoidiO05394.
KOiK17217.
OMAiFHQNAMG.
PhylomeDBiO05394.

Enzyme and pathway databases

BioCyciBSUB:BSU27250-MONOMER.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCCB_BACSU
AccessioniPrimary (citable) accession number: O05394
Secondary accession number(s): Q795Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 1, 1997
Last modified: October 5, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.