O05391 (BOFC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BofC

Alternative name(s):
Bypass-of-forespore protein C

Gene names

Name:	bofC
Ordered Locus Names:	BSU27750

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the spoIVB zymogen from undergoing autocatalytic activation by an unknown mechanism, and in this way plays a role in the sigma-K checkpoint of sporulation.
Subunit structure	Monomer. Ref.4
Subcellular location	Forespore intermembrane space.

Ontologies

Keywords
Biological process	Sporulation
Domain	Signal
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 30

30

Probable

Chain

31 – 170

140

Protein BofC

PRO_0000020824

Natural variations

Natural variant

96

1

S → F in BofC1.

Secondary structure

1

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170

Helix Strand Turn

Details...

Beta strand

34 – 48

15

Beta strand

50 – 52

3

Beta strand

54 – 65

12

Helix

66 – 72

7

Beta strand

76 – 82

7

Beta strand

85 – 91

7

Helix

98 – 100

3

Beta strand

105 – 108

4

Turn

109 – 111

3

Beta strand

112 – 119

8

Turn

135 – 137

3

Helix

140 – 148

9

Helix

155 – 168

14

Sequences

	Sequence		Length	Mass (Da)
	O05391 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 2786FEE1DBDFD2A2	FASTA	170	19,296
	10 20 30 40 50 60 MKRFSTAYLL LGILCSAAVF LIGAPSRALG AEVEHYEPLQ VHVQLEKVYL DGDVSIEHKH 70 80 90 100 110 120 EKVFSMDDFW AAYAGWTLVE QKKGYVLFRK QMDDISPLSK VNGYIGVSDN GVISTFHGRP 130 140 150 160 170 EPASEPIQSF FQIDLERLES HMQKNLLKGI PFRTKAEFED VIEHMKTYSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BofC encodes a putative forespore regulator of the Bacillus subtilis sigma K checkpoint." Gomez M., Cutting S.M. Microbiology 143:157-170(1997) [PubMed: 9025289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / PY79.
[2]	"A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis 168 chromosome: sequencing and ruv operon identification." Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"The structure of bypass of forespore C, an intercompartmental signaling factor during sporulation in Bacillus." Patterson H.M., Brannigan J.A., Cutting S.M., Wilson K.S., Wilkinson A.J., Ab E., Diercks T., de Jong R.N., Truffault V., Folkers G.E., Kaptein R. J. Biol. Chem. 280:36214-36220(2005) [PubMed: 16049010] [Abstract] Cited for: STRUCTURE BY NMR OF 31-170, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X93081 Genomic DNA. Translation: CAA63621.1.
Y15896 Genomic DNA. Translation: CAB75329.1.
AL009126 Genomic DNA. Translation: CAB14735.1.

PIR

A69596.

RefSeq

NP_390653.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BW2	NMR	-	A	31-170	[»]

ProteinModelPortal

O05391.

SMR

O05391. Positions 31-170.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000001756; EBBACP00000001756; EBBACG00000001754.

GeneID

937526.

GenomeReviews

Gene locus BSU27750 in contig AL009126_GR.

KEGG

bsu:BSU27750.

NMPDR

fig|224308.1.peg.2778.

PATRIC

18977418. VBIBacSub10457_2898.

Organism-specific databases

GenoList

BSU27750. [Micado]

Phylogenomic databases

GeneTree

EBGT00050000002298.

HOGENOM

HBG345153.

OMA

GVISTFH.

ProtClustDB

CLSK2518254.

Enzyme and pathway databases

BioCyc

BSUB:BSU27750-MONOMER.

Family and domain databases

InterPro

IPR015050. BofC_C.
IPR015071. BOFC_N.
[Graphical view]

KO

K06318.

Pfam

PF08955. BofC_C. 1 hit.
PF08977. BOFC_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BOFC_BACSU

Accession

Primary (citable) accession number: O05391

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program