ID   RPOH_BUCAI              Reviewed;         284 AA.
AC   O05385;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000255|HAMAP-Rule:MF_00961};
DE   AltName: Full=RNA polymerase sigma-32 factor {ECO:0000255|HAMAP-Rule:MF_00961};
GN   Name=rpoH {ECO:0000255|HAMAP-Rule:MF_00961}; OrderedLocusNames=BU025;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9079916; DOI=10.1128/jb.179.7.2300-2304.1997;
RA   Sato S., Ishikawa H.;
RT   "Expression and control of an operon from an intracellular symbiont which
RT   is homologous to the groE operon.";
RL   J. Bacteriol. 179:2300-2304(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is involved in regulation of expression of
CC       heat shock genes. {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00961}.
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DR   EMBL; U35400; AAB51433.1; -; Genomic_DNA.
DR   EMBL; BA000003; BAB12752.1; -; Genomic_DNA.
DR   RefSeq; NP_239866.1; NC_002528.1.
DR   RefSeq; WP_009873986.1; NC_002528.1.
DR   AlphaFoldDB; O05385; -.
DR   SMR; O05385; -.
DR   STRING; 563178.BUAP5A_025; -.
DR   EnsemblBacteria; BAB12752; BAB12752; BAB12752.
DR   KEGG; buc:BU025; -.
DR   PATRIC; fig|107806.10.peg.37; -.
DR   eggNOG; COG0568; Bacteria.
DR   HOGENOM; CLU_014793_3_5_6; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule.
DR   GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR   CDD; cd06171; Sigma70_r4; 1.
DR   Gene3D; 1.20.120.1810; -; 1.
DR   Gene3D; 1.20.140.160; -; 1.
DR   HAMAP; MF_00961; Sigma70_RpoH; 1.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR   NCBIfam; TIGR02392; rpoH_proteo; 1.
DR   NCBIfam; TIGR02937; sigma70-ECF; 1.
DR   PANTHER; PTHR30376:SF3; RNA POLYMERASE SIGMA FACTOR RPOH; 1.
DR   PANTHER; PTHR30376; SIGMA FACTOR RPOH HEAT SHOCK RELATED; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR   SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..284
FT                   /note="RNA polymerase sigma factor RpoH"
FT                   /id="PRO_0000093951"
FT   DNA_BIND        253..272
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          53..122
FT                   /note="Sigma-70 factor domain-2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   REGION          228..280
FT                   /note="Sigma-70 factor domain-4"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00961"
FT   MOTIF           77..80
FT                   /note="Interaction with polymerase core subunit RpoC"
SQ   SEQUENCE   284 AA;  32937 MW;  8BEE7BC9E0F18300 CRC64;
     MINKVQILSV TPPGNLDAYI RIANLWPMLS IEEEKKLTKR LRYNGDLDAA KTLILSHLRF
     VIHISRNYSG YGLLQSDLIQ EGNIGLMKAV RRFNPEIGVR LVSFAVHWIK SEIHEYVLRN
     WRIVKVATTK SQRKLFFNLR KTKKRLGWFN EEEIQIVARE LGVSSRDVRE MESRMSAQDV
     AFNPSPEEHC DSKTNSSIQY LQDKTSNFAN GVEQDNWEEH AANKLSSALL RLDERSRHII
     HARWLDKNKK NTLQNIANNY GISAERVRQL EKNAMKKLKL AIEA
//