Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O05385

- RPOH_BUCAI

UniProt

O05385 - RPOH_BUCAI

Protein

RNA polymerase sigma factor RpoH

Gene

rpoH

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi253 – 27220H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. sigma factor activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. response to heat Source: InterPro
    2. transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Sigma factor

    Keywords - Biological processi

    Stress response, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-25-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma factor RpoHUniRule annotation
    Alternative name(s):
    RNA polymerase sigma-32 factorUniRule annotation
    Gene namesi
    Name:rpoHUniRule annotation
    Ordered Locus Names:BU025
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284RNA polymerase sigma factor RpoHPRO_0000093951Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with the RNA polymerase core enzyme.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliO05385.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 12270Sigma-70 factor domain-2UniRule annotationAdd
    BLAST
    Regioni228 – 28053Sigma-70 factor domain-4UniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi77 – 804Interaction with polymerase core subunit RpoC

    Sequence similaritiesi

    Belongs to the sigma-70 factor family. RpoH subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0568.
    HOGENOMiHOG000270269.
    KOiK03089.
    OMAiEAANWEM.
    OrthoDBiEOG6D5G0W.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    HAMAPiMF_00961. Sigma70_RpoH.
    InterProiIPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012759. RNA_pol_sigma_RpoH_proteobac.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF04542. Sigma70_r2. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view]
    PRINTSiPR00046. SIGMA70FCT.
    SUPFAMiSSF88659. SSF88659. 1 hit.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsiTIGR02392. rpoH_proteo. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEiPS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O05385-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINKVQILSV TPPGNLDAYI RIANLWPMLS IEEEKKLTKR LRYNGDLDAA    50
    KTLILSHLRF VIHISRNYSG YGLLQSDLIQ EGNIGLMKAV RRFNPEIGVR 100
    LVSFAVHWIK SEIHEYVLRN WRIVKVATTK SQRKLFFNLR KTKKRLGWFN 150
    EEEIQIVARE LGVSSRDVRE MESRMSAQDV AFNPSPEEHC DSKTNSSIQY 200
    LQDKTSNFAN GVEQDNWEEH AANKLSSALL RLDERSRHII HARWLDKNKK 250
    NTLQNIANNY GISAERVRQL EKNAMKKLKL AIEA 284
    Length:284
    Mass (Da):32,937
    Last modified:July 1, 1997 - v1
    Checksum:i8BEE7BC9E0F18300
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35400 Genomic DNA. Translation: AAB51433.1.
    BA000003 Genomic DNA. Translation: BAB12752.1.
    RefSeqiNP_239866.1. NC_002528.1.
    WP_009873986.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12752; BAB12752; BAB12752.
    GeneIDi1109533.
    KEGGibuc:BU025.
    PATRICi21243554. VBIBucAph127364_0037.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35400 Genomic DNA. Translation: AAB51433.1 .
    BA000003 Genomic DNA. Translation: BAB12752.1 .
    RefSeqi NP_239866.1. NC_002528.1.
    WP_009873986.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali O05385.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12752 ; BAB12752 ; BAB12752 .
    GeneIDi 1109533.
    KEGGi buc:BU025.
    PATRICi 21243554. VBIBucAph127364_0037.

    Phylogenomic databases

    eggNOGi COG0568.
    HOGENOMi HOG000270269.
    KOi K03089.
    OMAi EAANWEM.
    OrthoDBi EOG6D5G0W.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-25-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    HAMAPi MF_00961. Sigma70_RpoH.
    InterProi IPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012759. RNA_pol_sigma_RpoH_proteobac.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF04542. Sigma70_r2. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view ]
    PRINTSi PR00046. SIGMA70FCT.
    SUPFAMi SSF88659. SSF88659. 1 hit.
    SSF88946. SSF88946. 1 hit.
    TIGRFAMsi TIGR02392. rpoH_proteo. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEi PS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and control of an operon from an intracellular symbiont which is homologous to the groE operon."
      Sato S., Ishikawa H.
      J. Bacteriol. 179:2300-2304(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiRPOH_BUCAI
    AccessioniPrimary (citable) accession number: O05385
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3