Reviewed, UniProtKB/Swiss-Prot O05385 (RP32_BUCAI)

Names and origin

Protein names

Recommended name:
    RNA polymerase sigma-32 factor

Gene names

Name:	rpoH
Ordered Locus Names:	BU025

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters By similarity.
Sequence similarities	Belongs to the sigma-70 factor family. Sigma-32 subfamily.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to heat Inferred from electronic annotation. Source: InterPro transcription initiation Inferred from electronic annotation. Source: InterPro
Molecular function	sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	RNA polymerase sigma-32 factor		PRO_0000093951
Regions
	DNA binding	253 – 272	20	H-T-H motif By similarity
	Motif	77 – 90	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	O05385-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 8BEE7BC9E0F18300	FASTA	284	32,937
	10 20 30 40 50 60 MINKVQILSV TPPGNLDAYI RIANLWPMLS IEEEKKLTKR LRYNGDLDAA KTLILSHLRF 70 80 90 100 110 120 VIHISRNYSG YGLLQSDLIQ EGNIGLMKAV RRFNPEIGVR LVSFAVHWIK SEIHEYVLRN 130 140 150 160 170 180 WRIVKVATTK SQRKLFFNLR KTKKRLGWFN EEEIQIVARE LGVSSRDVRE MESRMSAQDV 190 200 210 220 230 240 AFNPSPEEHC DSKTNSSIQY LQDKTSNFAN GVEQDNWEEH AANKLSSALL RLDERSRHII 250 260 270 280 HARWLDKNKK NTLQNIANNY GISAERVRQL EKNAMKKLKL AIEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and control of an operon from an intracellular symbiont which is homologous to the groE operon." Sato S., Ishikawa H. J. Bacteriol. 179:2300-2304(1997) [PubMed: 9079916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U35400 Genomic DNA. Translation: AAB51433.1. BA000003 Genomic DNA. Translation: BAB12752.1.
RefSeq	NP_239866.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109533.
GenomeReviews	Gene locus BU025 in contig BA000003_GR.
KEGG	buc:BU025.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O05385.
OMA	SEINRFP
Enzyme and pathway databases
BioCyc	BSP107806:BU025-MON.
Family and domain databases
InterPro	IPR014284. RNA_pol_sigma-70. IPR000943. RNA_pol_sigma70. IPR007627. RNA_pol_sigma70_r2. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012759. RNA_pol_sigma_RpoH_proteobac. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF04542. Sigma70_r2. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
TIGRFAMs	TIGR02392. rpoH_proteo. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RP32_BUCAI

Accession

Primary (citable) accession number: O05385

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)