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Protein

RNA polymerase sigma factor RpoH

Gene

rpoH

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi253 – 27220H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. sigma factor activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. response to heat Source: InterPro
  2. transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-25-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoHUniRule annotation
Alternative name(s):
RNA polymerase sigma-32 factorUniRule annotation
Gene namesi
Name:rpoHUniRule annotation
Ordered Locus Names:BU025
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284RNA polymerase sigma factor RpoHPRO_0000093951Add
BLAST

Interactioni

Subunit structurei

Interacts with the RNA polymerase core enzyme.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO05385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 12270Sigma-70 factor domain-2UniRule annotationAdd
BLAST
Regioni228 – 28053Sigma-70 factor domain-4UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 804Interaction with polymerase core subunit RpoC

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoH subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0568.
HOGENOMiHOG000270269.
KOiK03089.
OMAiENFETQA.
OrthoDBiEOG6D5G0W.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00961. Sigma70_RpoH.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012759. RNA_pol_sigma_RpoH_proteobac.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 1 hit.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02392. rpoH_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05385-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINKVQILSV TPPGNLDAYI RIANLWPMLS IEEEKKLTKR LRYNGDLDAA
60 70 80 90 100
KTLILSHLRF VIHISRNYSG YGLLQSDLIQ EGNIGLMKAV RRFNPEIGVR
110 120 130 140 150
LVSFAVHWIK SEIHEYVLRN WRIVKVATTK SQRKLFFNLR KTKKRLGWFN
160 170 180 190 200
EEEIQIVARE LGVSSRDVRE MESRMSAQDV AFNPSPEEHC DSKTNSSIQY
210 220 230 240 250
LQDKTSNFAN GVEQDNWEEH AANKLSSALL RLDERSRHII HARWLDKNKK
260 270 280
NTLQNIANNY GISAERVRQL EKNAMKKLKL AIEA
Length:284
Mass (Da):32,937
Last modified:July 1, 1997 - v1
Checksum:i8BEE7BC9E0F18300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35400 Genomic DNA. Translation: AAB51433.1.
BA000003 Genomic DNA. Translation: BAB12752.1.
RefSeqiNP_239866.1. NC_002528.1.
WP_009873986.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12752; BAB12752; BAB12752.
GeneIDi1109533.
KEGGibuc:BU025.
PATRICi21243554. VBIBucAph127364_0037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35400 Genomic DNA. Translation: AAB51433.1.
BA000003 Genomic DNA. Translation: BAB12752.1.
RefSeqiNP_239866.1. NC_002528.1.
WP_009873986.1. NC_002528.1.

3D structure databases

ProteinModelPortaliO05385.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12752; BAB12752; BAB12752.
GeneIDi1109533.
KEGGibuc:BU025.
PATRICi21243554. VBIBucAph127364_0037.

Phylogenomic databases

eggNOGiCOG0568.
HOGENOMiHOG000270269.
KOiK03089.
OMAiENFETQA.
OrthoDBiEOG6D5G0W.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-25-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00961. Sigma70_RpoH.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012759. RNA_pol_sigma_RpoH_proteobac.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 1 hit.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02392. rpoH_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression and control of an operon from an intracellular symbiont which is homologous to the groE operon."
    Sato S., Ishikawa H.
    J. Bacteriol. 179:2300-2304(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiRPOH_BUCAI
AccessioniPrimary (citable) accession number: O05385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.