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O05385 (RPOH_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase sigma factor RpoH
Alternative name(s):
RNA polymerase sigma-32 factor
Gene names
Name:rpoH
Ordered Locus Names:BU025
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes By similarity. HAMAP-Rule MF_00961

Subunit structure

Interacts with the RNA polymerase core enzyme By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00961.

Sequence similarities

Belongs to the sigma-70 factor family. RpoH subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284RNA polymerase sigma factor RpoH HAMAP-Rule MF_00961
PRO_0000093951

Regions

DNA binding253 – 27220H-T-H motif By similarity
Region53 – 12270Sigma-70 factor domain-2 By similarity
Region228 – 28053Sigma-70 factor domain-4 By similarity
Motif77 – 804Interaction with polymerase core subunit RpoC HAMAP-Rule MF_00961

Sequences

Sequence LengthMass (Da)Tools
O05385 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 8BEE7BC9E0F18300

FASTA28432,937
        10         20         30         40         50         60 
MINKVQILSV TPPGNLDAYI RIANLWPMLS IEEEKKLTKR LRYNGDLDAA KTLILSHLRF 

        70         80         90        100        110        120 
VIHISRNYSG YGLLQSDLIQ EGNIGLMKAV RRFNPEIGVR LVSFAVHWIK SEIHEYVLRN 

       130        140        150        160        170        180 
WRIVKVATTK SQRKLFFNLR KTKKRLGWFN EEEIQIVARE LGVSSRDVRE MESRMSAQDV 

       190        200        210        220        230        240 
AFNPSPEEHC DSKTNSSIQY LQDKTSNFAN GVEQDNWEEH AANKLSSALL RLDERSRHII 

       250        260        270        280 
HARWLDKNKK NTLQNIANNY GISAERVRQL EKNAMKKLKL AIEA 

« Hide

References

« Hide 'large scale' references
[1]"Expression and control of an operon from an intracellular symbiont which is homologous to the groE operon."
Sato S., Ishikawa H.
J. Bacteriol. 179:2300-2304(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35400 Genomic DNA. Translation: AAB51433.1.
BA000003 Genomic DNA. Translation: BAB12752.1.
RefSeqNP_239866.1. NC_002528.1.

3D structure databases

ProteinModelPortalO05385.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12752; BAB12752; BAB12752.
GeneID1109533.
KEGGbuc:BU025.
PATRIC21243554. VBIBucAph127364_0037.

Phylogenomic databases

eggNOGCOG0568.
HOGENOMHOG000270269.
KOK03089.
OMAEAANWEM.
OrthoDBEOG6D5G0W.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-25-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_00961. Sigma70_RpoH.
InterProIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012759. RNA_pol_sigma_RpoH_proteobac.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF04542. Sigma70_r2. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSPR00046. SIGMA70FCT.
SUPFAMSSF88659. SSF88659. 1 hit.
SSF88946. SSF88946. 1 hit.
TIGRFAMsTIGR02392. rpoH_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOH_BUCAI
AccessionPrimary (citable) accession number: O05385
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names