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O05322

- DAPF_PSEFL

UniProt

O05322 - DAPF_PSEFL

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Protein
Diaminopimelate epimerase
Gene
dapF
Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Substrate By similarity
Binding sitei46 – 461Substrate By similarity
Binding sitei66 – 661Substrate By similarity
Active sitei75 – 751Proton donor/acceptor By similarity
Binding sitei159 – 1591Substrate By similarity
Sitei161 – 1611Important for catalytic activity By similarity
Binding sitei192 – 1921Substrate By similarity
Sitei210 – 2101Important for catalytic activity By similarity
Active sitei219 – 2191Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Diaminopimelate epimeraseUniRule annotation
PRO_0000149860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 219UniRule annotation

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliO05322.
SMRiO05322. Positions 3-276.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Substrate binding By similarity
Regioni75 – 773Substrate binding By similarity
Regioni210 – 2112Substrate binding By similarity
Regioni220 – 2212Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O05322-1 [UniParc]FASTAAdd to Basket

« Hide

MLLRFTKMHG LGNDFMVLDL VSQHAHILPK HAKQWGDRHT GIGFDQLLIV    50
EAPNNPDVDF RYRIFNADGS EVEQCGNGAR CFARFVLDKR LTAKRQIRVE 100
TKSGIIELDV RSDGQIGVNM GAPRLVPADI PFQAPAQALS YQVDVDGATV 150
ELAAVSMGNP HAVLRVADIN SAPVHELGPK IEHHPRFPAR VNVGFLQVID 200
RHRAQLRVWE RGAGETQACG TGACAAAVAA ISQGWMDSPL LIDLPGGRLS 250
IEWAGPGQPV MMTGPAVRVY EGQVRL 276
Length:276
Mass (Da):30,070
Last modified:July 1, 1997 - v1
Checksum:i3E8E2AF0D5B46835
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12268 Genomic DNA. Translation: CAA72944.1.
PIRiT10459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12268 Genomic DNA. Translation: CAA72944.1 .
PIRi T10459.

3D structure databases

ProteinModelPortali O05322.
SMRi O05322. Positions 3-276.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A site-specific recombinase is required for competitive root colonization by Pseudomonas fluorescens WCS365."
    Dekkers L.C., Phoelich C.C., van der Fits L., Lugtenberg B.J.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:7051-7056(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WCS365.

Entry informationi

Entry nameiDAPF_PSEFL
AccessioniPrimary (citable) accession number: O05322
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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