O05313 (GLGA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsular glucan synthase
EC=2.4.1.21

Alternative name(s):
ADP-glucose--glucan glucosyltransferase
Alpha-1,4-glucosyltransferase GlgA
Extracellular alpha-D-glucan synthase

Gene names

Name:	glgA
Ordered Locus Names:	Rv1212c, MT1250

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glucosyltransferase that uses ADP-glucose as the sugar donor to elongate alpha-glucans Probable. Is involved in the production of the capsular alpha-D-glucan. Ref.3
Catalytic activity	ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).
Pathway	Capsule biogenesis; capsule polysaccharide biosynthesis. Ref.3
Disruption phenotype	Inactivation of glgA affects the production of extracellular alpha-D-glucan (two-fold reduction), but not that of intracellular glycogen and 6-O-methylglucosyl lipopolysaccharides (MGLP). Cells lacking this gene are also impaired in their ability to persist in both the spleen and the lungs of mice. Ref.3
Miscellaneous	Attempts to disrupt both the Rv3032 gene and glgA in order to create a mutant simultaneously deficient in both alpha-1,4-glucosyltransferases turned out to be unsuccessful. Thus, M.tuberculosis H37Rv requires a functional copy of at least one of these two genes for growth.
Sequence similarities	Belongs to the glycosyltransferase group 1 family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glucan biosynthetic process Inferred from mutant phenotype Ref.3. Source: MTBBASE
Molecular_function	starch synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Capsular glucan synthase		PRO_0000413978

Sequences

	Sequence		Length	Mass (Da)
	O05313 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: BC4C4B66980BAAFF	FASTA	387	41,533
	10 20 30 40 50 60 MRVAMLTREY PPEVYGGAGV HVTELVAYLR RLCAVDVHCM GAPRPGAFAY RPDPRLGSAN 70 80 90 100 110 120 AALSTLSADL VMANAASAAT VVHSHTWYTA LAGHLAAILY DIPHVLTAHS LEPLRPWKKE 130 140 150 160 170 180 QLGGGYQVST WVEQTAVLAA NAVIAVSSAM RNDMLRVYPS LDPNLVHVIR NGIDTETWYP 190 200 210 220 230 240 AGPARTGSVL AELGVDPNRP MAVFVGRITR QKGVVHLVTA AHRFRSDVQL VLCAGAADTP 250 260 270 280 290 300 EVADEVRVAV AELARNRTGV FWIQDRLTIG QLREILSAAT VFVCPSVYEP LGIVNLEAMA 310 320 330 340 350 360 CATAVVASDV GGIPEVVADG ITGSLVHYDA DDATGYQARL AEAVNALVAD PATAERYGHA 370 380 GRQRCIQEFS WAYIAEQTLD IYRKVCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice." Sambou T., Dinadayala P., Stadthagen G., Barilone N., Bordat Y., Constant P., Levillain F., Neyrolles O., Gicquel B., Lemassu A., Daffe M., Jackson M. Mol. Microbiol. 70:762-774(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CAPSULAR GLUCAN BIOSYNTHESIS, DISRUPTION PHENOTYPE, PATHWAY. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842575 Genomic DNA. Translation: CAB07814.1. AE000516 Genomic DNA. Translation: AAK45507.1. AL123456 Genomic DNA. Translation: CCP43968.1.
PIR	B70610.
RefSeq	NP_215728.1. NC_000962.3. NP_335693.1. NC_002755.2. YP_006514587.1. NC_018143.1.
3D structure databases
ProteinModelPortal	O05313.
SMR	O05313. Positions 81-387.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv1212c.
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK45507; AAK45507; MT1250.
GeneID	13319791. 887805. 924818.
KEGG	mtc:MT1250. mtu:Rv1212c. mtv:RVBD_1212c.
PATRIC	18124516. VBIMycTub22151_1377.
Organism-specific databases
TubercuList	Rv1212c.
Phylogenomic databases
HOGENOM	HOG000077288.
KO	K16148.
OMA	HGTPLVI.
ProtClustDB	CLSK871909.
Enzyme and pathway databases
UniPathway	UPA00934.
Family and domain databases
InterPro	IPR011875. GlgA_Coryne_bac. IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02149. glgA_Coryne. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGA_MYCTU

Accession

Primary (citable) accession number: O05313
Secondary accession number(s): L0T8Z4, Q7D8L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program