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O05272 (ASNO_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing] 3

EC=6.3.5.4
Gene names
Name:asnO
Synonyms:yisO, yucB
Ordered Locus Names:BSU10790
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Asparagine synthetase involved in sporulation.

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence caution

The sequence CAA70643.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 614613Asparagine synthetase [glutamine-hydrolyzing] 3
PRO_0000056934

Regions

Domain2 – 216215Glutamine amidotransferase type-2
Nucleotide binding377 – 3782ATP By similarity
Region50 – 545Glutamine binding By similarity
Region77 – 793Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1021Glutamine By similarity
Site3791Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
O05272 [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: D621D9A4557A8AC1

FASTA61470,714
        10         20         30         40         50         60 
MCGITGWVDF KKQLVQEKQT MDRMTDTLSK RGPDDSNVWG EHHVLFGHKR LAVVDIEGGR 

        70         80         90        100        110        120 
QPMACTYKGD TYTIIYNGEL YNTEDLRKEL RARGHQFERT SDTEVLLHSY IEWQEDCVDH 

       130        140        150        160        170        180 
LNGIFAFAVW DEKRNLLFAA RDRLGVKPFF YTKEGSSFLF GSEIKAILAH PDIKARVDRT 

       190        200        210        220        230        240 
GLSEIFGLGP SRTPGTGIFK GIKEIRPAHA LTFSKDGLNI WRYWNVESEK HTDSFDDTVA 

       250        260        270        280        290        300 
NVRSLFQDAV TRQLVSDVPV CTFLSGGLDS SAITAIAAGH FEKEGKAPLH TYSIDYEEND 

       310        320        330        340        350        360 
KYFQASAFQP NDDGPWIEKM TEAFGTTHHK CVISQKDLVD HLEEAVLVKD LPGMADVDSS 

       370        380        390        400        410        420 
LLWFCREIKK DFVVSLSGEC ADEIFGGYPW FHTADVESGF PWMRSTEERI KLLSDSWQKK 

       430        440        450        460        470        480 
LNLKEYVNAK YEETLAETPL LDGETGVDKA RRQLFYLNML WFMTNLLDRK DRMSMGASLE 

       490        500        510        520        530        540 
VRVPFADHRL VEYVWNIPWE MKMHDNREKG ILRKALEGIL PDDILYRKKS PYPKTHHPEY 

       550        560        570        580        590        600 
TKGVSEWLKT IRSQKDSVLH TLLDRKQLDQ LLETEGSSFK VPWFGQLMKG PQLIAHLAQI 

       610 
HTWFEAYRID IDER 

« Hide

References

« Hide 'large scale' references
[1]"Bacillus subtilis genome project, DNA sequence from yucA to yucH."
Oudega B., Koningstein G., Duesterhoeft A.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis."
Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J.
Microbiology 143:3305-3308(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Three asparagine synthetase genes of Bacillus subtilis."
Yoshida K., Fujita Y., Ehrlich S.D.
J. Bacteriol. 181:6081-6091(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z93940 Genomic DNA. Translation: CAB07965.1.
Y09476 Genomic DNA. Translation: CAA70643.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB12919.2.
PIRF69837.
RefSeqNP_388960.2. NC_000964.3.

3D structure databases

ProteinModelPortalO05272.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU10790.

Proteomic databases

PaxDbO05272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12919; CAB12919; BSU10790.
GeneID939783.
KEGGbsu:BSU10790.
PATRIC18973862. VBIBacSub10457_1125.

Organism-specific databases

GenoListBSU10790. [Micado]

Phylogenomic databases

eggNOGCOG0367.
HOGENOMHOG000027494.
KOK01953.
OMAFGQLMSG.
OrthoDBEOG6Q5NPQ.
PhylomeDBO05272.

Enzyme and pathway databases

BioCycBSUB:BSU10790-MONOMER.
UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNO_BACSU
AccessionPrimary (citable) accession number: O05272
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList