Reviewed, UniProtKB/Swiss-Prot O05269 (GUAC_BACSU)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Synonyms:	yumD
Ordered Locus Names:	BSU32130

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides Probable. Ref.4
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Induction	Expression is regulated by purR. Ref.4
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	GMP reductase HAMAP MF_01511		PRO_0000093752
Regions
	Nucleotide binding	204 – 227	24	NADP By similarity
Sites
	Active site	175	1	Thioimidate intermediate By similarity
Experimental info
	Sequence conflict	262	1	Q → P in CAB07955. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O05269-1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: 4CEF76F398E1D353	FASTA	326	35,850
	10 20 30 40 50 60 MENVFDYEDI QLIPAKCIVN SRSECDTSVR LGGHTFKLPV VPANMQTIID EKLAISLAEN 70 80 90 100 110 120 GYFYVMHRFE PETRIDFIKD MNARGLFSSI SVGVKDEEYE FVRQLAEENL TPEYVTIDIA 130 140 150 160 170 180 HGHSNAVIEM IQHLKKHLPD SFVIAGNVGT PEAVRELENA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTHGDIAKSI RFGATMVMIG SLFAGHEESP 250 260 270 280 290 300 GQTIEKDGKL YKEYFGSASE FQKGEKKNVE GKKMHVAHKG SIKDTLIEME QDLQSSISYA 310 320 GGTKLNAIRN VDYVIVKNSI FNGDKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Oudega B., Koningstein G., de Sales Ramon M., Rodrigues L. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 262.
[4]	"Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO." Saxild H.H., Brunstedt K., Nielsen K.I., Jarmer H., Nygaard P. J. Bacteriol. 183:6175-6183(2001) [PubMed: 11591660] [Abstract] Cited for: PROBABLE FUNCTION, REGULATION OF EXPRESSION. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z93939 Genomic DNA. Translation: CAB07955.1. AL009126 Genomic DNA. Translation: CAB15203.2.
PIR	C70015.
RefSeq	NP_391093.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937189.
GenomeReviews	Gene locus BSU32130 in contig AL009126_GR.
KEGG	bsu:BSU32130.
NMPDR	fig|224308.1.peg.3219.
Organism-specific databases
SubtiList	BG12392. guaC. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
PhylomeDB	O05269.
Enzyme and pathway databases
BioCyc	SUBTI:BSU32130-MONOMER.
BRENDA	1.7.1.7. 150.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACSU

Accession

Primary (citable) accession number: O05269

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	July 28, 2009
Last modified:	February 9, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)