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Protein

Ferredoxin--NADP reductase 2

Gene

yumC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Kineticsi

KM for NADH is < 0.1 µM.

  1. KM=0.54 µM for NADPH (at 24 degrees Celsius and pH 7.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371FADBy similarity
    Binding sitei45 – 451FADBy similarity
    Binding sitei50 – 501FADBy similarity
    Binding sitei90 – 901FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei124 – 1241FAD; via amide nitrogenBy similarity
    Binding sitei285 – 2851FADBy similarity
    Binding sitei326 – 3261FADBy similarity

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciBSUB:BSU32110-MONOMER.
    BRENDAi1.18.1.2. 658.
    SABIO-RKO05268.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferredoxin--NADP reductase 2 (EC:1.18.1.2)
    Short name:
    FNR 2
    Short name:
    Fd-NADP(+) reductase 2
    Gene namesi
    Name:yumC
    Ordered Locus Names:BSU32110
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Ferredoxin--NADP reductase 2PRO_0000360548Add
    BLAST

    Proteomic databases

    PaxDbiO05268.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiO05268. 1 interaction.
    MINTiMINT-8366158.
    STRINGi224308.Bsubs1_010100017431.

    Structurei

    Secondary structure

    1
    332
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312Combined sources
    Helixi17 – 2812Combined sources
    Beta strandi33 – 364Combined sources
    Beta strandi38 – 425Combined sources
    Helixi44 – 496Combined sources
    Beta strandi53 – 553Combined sources
    Beta strandi62 – 654Combined sources
    Helixi66 – 7712Combined sources
    Beta strandi83 – 853Combined sources
    Beta strandi90 – 956Combined sources
    Beta strandi101 – 11717Combined sources
    Beta strandi122 – 1265Combined sources
    Helixi134 – 1374Combined sources
    Turni139 – 1413Combined sources
    Beta strandi142 – 1454Combined sources
    Helixi149 – 1524Combined sources
    Beta strandi156 – 1605Combined sources
    Helixi164 – 17310Combined sources
    Turni174 – 1763Combined sources
    Beta strandi177 – 1837Combined sources
    Beta strandi185 – 1884Combined sources
    Helixi193 – 2019Combined sources
    Beta strandi205 – 2073Combined sources
    Beta strandi210 – 2167Combined sources
    Beta strandi218 – 2203Combined sources
    Beta strandi223 – 2286Combined sources
    Beta strandi234 – 2385Combined sources
    Beta strandi240 – 2445Combined sources
    Beta strandi248 – 2514Combined sources
    Helixi253 – 2575Combined sources
    Beta strandi266 – 2683Combined sources
    Beta strandi280 – 2823Combined sources
    Helixi284 – 2863Combined sources
    Helixi296 – 31419Combined sources
    Helixi325 – 3284Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LZWX-ray1.80A1-332[»]
    3LZXX-ray1.90A/B1-332[»]
    ProteinModelPortaliO05268.
    SMRiO05268. Positions 1-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO05268.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CAY. Bacteria.
    COG0492. LUCA.
    HOGENOMiHOG000072909.
    InParanoidiO05268.
    KOiK00384.
    OMAiSETHYSK.
    PhylomeDBiO05268.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    HAMAPiMF_01685. FENR2. 1 hit.
    InterProiIPR023753. FAD/NAD-binding_dom.
    IPR022890. Fd--NADP_Rdtase_type_2.
    IPR000103. Pyridine_nuc-diS_OxRdtase_2.
    [Graphical view]
    PfamiPF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PRINTSiPR00469. PNDRDTASEII.
    SUPFAMiSSF51905. SSF51905. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O05268-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MREDTKVYDI TIIGGGPVGL FTAFYGGMRQ ASVKIIESLP QLGGQLSALY
    60 70 80 90 100
    PEKYIYDVAG FPKIRAQELI NNLKEQMAKF DQTICLEQAV ESVEKQADGV
    110 120 130 140 150
    FKLVTNEETH YSKTVIITAG NGAFKPRKLE LENAEQYEGK NLHYFVDDLQ
    160 170 180 190 200
    KFAGRRVAIL GGGDSAVDWA LMLEPIAKEV SIIHRRDKFR AHEHSVENLH
    210 220 230 240 250
    ASKVNVLTPF VPAELIGEDK IEQLVLEEVK GDRKEILEID DLIVNYGFVS
    260 270 280 290 300
    SLGPIKNWGL DIEKNSIVVK STMETNIEGF FAAGDICTYE GKVNLIASGF
    310 320 330
    GEAPTAVNNA KAYMDPKARV QPLHSTSLFE NK
    Length:332
    Mass (Da):36,841
    Last modified:July 28, 2009 - v2
    Checksum:iE84835A1861A1529
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 11612TNEET…SKTVI → QMKKPTTLKRSC in CAB07954 (Ref. 1) CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z93939 Genomic DNA. Translation: CAB07954.1.
    AL009126 Genomic DNA. Translation: CAB15201.2.
    PIRiB70015.
    RefSeqiNP_391091.2. NC_000964.3.
    WP_003220618.1. NZ_JNCM01000033.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15201; CAB15201; BSU32110.
    GeneIDi11240812.
    936577.
    KEGGibsu:BSU32110.
    PATRICi18978394. VBIBacSub10457_3360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z93939 Genomic DNA. Translation: CAB07954.1.
    AL009126 Genomic DNA. Translation: CAB15201.2.
    PIRiB70015.
    RefSeqiNP_391091.2. NC_000964.3.
    WP_003220618.1. NZ_JNCM01000033.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LZWX-ray1.80A1-332[»]
    3LZXX-ray1.90A/B1-332[»]
    ProteinModelPortaliO05268.
    SMRiO05268. Positions 1-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiO05268. 1 interaction.
    MINTiMINT-8366158.
    STRINGi224308.Bsubs1_010100017431.

    Proteomic databases

    PaxDbiO05268.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15201; CAB15201; BSU32110.
    GeneIDi11240812.
    936577.
    KEGGibsu:BSU32110.
    PATRICi18978394. VBIBacSub10457_3360.

    Phylogenomic databases

    eggNOGiENOG4105CAY. Bacteria.
    COG0492. LUCA.
    HOGENOMiHOG000072909.
    InParanoidiO05268.
    KOiK00384.
    OMAiSETHYSK.
    PhylomeDBiO05268.

    Enzyme and pathway databases

    BioCyciBSUB:BSU32110-MONOMER.
    BRENDAi1.18.1.2. 658.
    SABIO-RKO05268.

    Miscellaneous databases

    EvolutionaryTraceiO05268.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    HAMAPiMF_01685. FENR2. 1 hit.
    InterProiIPR023753. FAD/NAD-binding_dom.
    IPR022890. Fd--NADP_Rdtase_type_2.
    IPR000103. Pyridine_nuc-diS_OxRdtase_2.
    [Graphical view]
    PfamiPF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PRINTSiPR00469. PNDRDTASEII.
    SUPFAMiSSF51905. SSF51905. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFENR2_BACSU
    AccessioniPrimary (citable) accession number: O05268
    Secondary accession number(s): Q795K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 28, 2009
    Last modified: September 7, 2016
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.