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Protein

Ferredoxin--NADP reductase 2

Gene

yumC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.1 Publication

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Kineticsi

KM for NADH is < 0.1 µM.

  1. KM=0.54 µM for NADPH (at 24 degrees Celsius and pH 7.0)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei37FADBy similarity1
    Binding sitei45FADBy similarity1
    Binding sitei50FADBy similarity1
    Binding sitei90FAD; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei124FAD; via amide nitrogenBy similarity1
    Binding sitei285FADBy similarity1
    Binding sitei326FADBy similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciBSUB:BSU32110-MONOMER.
    BRENDAi1.18.1.2. 658.
    SABIO-RKO05268.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferredoxin--NADP reductase 2 (EC:1.18.1.2)
    Short name:
    FNR 2
    Short name:
    Fd-NADP(+) reductase 2
    Gene namesi
    Name:yumC
    Ordered Locus Names:BSU32110
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003605481 – 332Ferredoxin--NADP reductase 2Add BLAST332

    Proteomic databases

    PaxDbiO05268.
    PRIDEiO05268.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiO05268. 1 interactor.
    MINTiMINT-8366158.
    STRINGi224308.Bsubs1_010100017431.

    Structurei

    Secondary structure

    1332
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 13Combined sources12
    Helixi17 – 28Combined sources12
    Beta strandi33 – 36Combined sources4
    Beta strandi38 – 42Combined sources5
    Helixi44 – 49Combined sources6
    Beta strandi53 – 55Combined sources3
    Beta strandi62 – 65Combined sources4
    Helixi66 – 77Combined sources12
    Beta strandi83 – 85Combined sources3
    Beta strandi90 – 95Combined sources6
    Beta strandi101 – 117Combined sources17
    Beta strandi122 – 126Combined sources5
    Helixi134 – 137Combined sources4
    Turni139 – 141Combined sources3
    Beta strandi142 – 145Combined sources4
    Helixi149 – 152Combined sources4
    Beta strandi156 – 160Combined sources5
    Helixi164 – 173Combined sources10
    Turni174 – 176Combined sources3
    Beta strandi177 – 183Combined sources7
    Beta strandi185 – 188Combined sources4
    Helixi193 – 201Combined sources9
    Beta strandi205 – 207Combined sources3
    Beta strandi210 – 216Combined sources7
    Beta strandi218 – 220Combined sources3
    Beta strandi223 – 228Combined sources6
    Beta strandi234 – 238Combined sources5
    Beta strandi240 – 244Combined sources5
    Beta strandi248 – 251Combined sources4
    Helixi253 – 257Combined sources5
    Beta strandi266 – 268Combined sources3
    Beta strandi280 – 282Combined sources3
    Helixi284 – 286Combined sources3
    Helixi296 – 314Combined sources19
    Helixi325 – 328Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3LZWX-ray1.80A1-332[»]
    3LZXX-ray1.90A/B1-332[»]
    ProteinModelPortaliO05268.
    SMRiO05268.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO05268.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CAY. Bacteria.
    COG0492. LUCA.
    HOGENOMiHOG000072909.
    InParanoidiO05268.
    KOiK00384.
    OMAiSETHYSK.
    PhylomeDBiO05268.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    HAMAPiMF_01685. FENR2. 1 hit.
    InterProiIPR023753. FAD/NAD-binding_dom.
    IPR022890. Fd--NADP_Rdtase_type_2.
    IPR000103. Pyridine_nuc-diS_OxRdtase_2.
    [Graphical view]
    PfamiPF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PRINTSiPR00469. PNDRDTASEII.
    SUPFAMiSSF51905. SSF51905. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O05268-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MREDTKVYDI TIIGGGPVGL FTAFYGGMRQ ASVKIIESLP QLGGQLSALY
    60 70 80 90 100
    PEKYIYDVAG FPKIRAQELI NNLKEQMAKF DQTICLEQAV ESVEKQADGV
    110 120 130 140 150
    FKLVTNEETH YSKTVIITAG NGAFKPRKLE LENAEQYEGK NLHYFVDDLQ
    160 170 180 190 200
    KFAGRRVAIL GGGDSAVDWA LMLEPIAKEV SIIHRRDKFR AHEHSVENLH
    210 220 230 240 250
    ASKVNVLTPF VPAELIGEDK IEQLVLEEVK GDRKEILEID DLIVNYGFVS
    260 270 280 290 300
    SLGPIKNWGL DIEKNSIVVK STMETNIEGF FAAGDICTYE GKVNLIASGF
    310 320 330
    GEAPTAVNNA KAYMDPKARV QPLHSTSLFE NK
    Length:332
    Mass (Da):36,841
    Last modified:July 28, 2009 - v2
    Checksum:iE84835A1861A1529
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti105 – 116TNEET…SKTVI → QMKKPTTLKRSC in CAB07954 (Ref. 1) CuratedAdd BLAST12

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z93939 Genomic DNA. Translation: CAB07954.1.
    AL009126 Genomic DNA. Translation: CAB15201.2.
    PIRiB70015.
    RefSeqiNP_391091.2. NC_000964.3.
    WP_003220618.1. NZ_JNCM01000033.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15201; CAB15201; BSU32110.
    GeneIDi11240812.
    936577.
    KEGGibsu:BSU32110.
    PATRICi18978394. VBIBacSub10457_3360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z93939 Genomic DNA. Translation: CAB07954.1.
    AL009126 Genomic DNA. Translation: CAB15201.2.
    PIRiB70015.
    RefSeqiNP_391091.2. NC_000964.3.
    WP_003220618.1. NZ_JNCM01000033.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3LZWX-ray1.80A1-332[»]
    3LZXX-ray1.90A/B1-332[»]
    ProteinModelPortaliO05268.
    SMRiO05268.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiO05268. 1 interactor.
    MINTiMINT-8366158.
    STRINGi224308.Bsubs1_010100017431.

    Proteomic databases

    PaxDbiO05268.
    PRIDEiO05268.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB15201; CAB15201; BSU32110.
    GeneIDi11240812.
    936577.
    KEGGibsu:BSU32110.
    PATRICi18978394. VBIBacSub10457_3360.

    Phylogenomic databases

    eggNOGiENOG4105CAY. Bacteria.
    COG0492. LUCA.
    HOGENOMiHOG000072909.
    InParanoidiO05268.
    KOiK00384.
    OMAiSETHYSK.
    PhylomeDBiO05268.

    Enzyme and pathway databases

    BioCyciBSUB:BSU32110-MONOMER.
    BRENDAi1.18.1.2. 658.
    SABIO-RKO05268.

    Miscellaneous databases

    EvolutionaryTraceiO05268.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    HAMAPiMF_01685. FENR2. 1 hit.
    InterProiIPR023753. FAD/NAD-binding_dom.
    IPR022890. Fd--NADP_Rdtase_type_2.
    IPR000103. Pyridine_nuc-diS_OxRdtase_2.
    [Graphical view]
    PfamiPF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PRINTSiPR00469. PNDRDTASEII.
    SUPFAMiSSF51905. SSF51905. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFENR2_BACSU
    AccessioniPrimary (citable) accession number: O05268
    Secondary accession number(s): Q795K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: July 28, 2009
    Last modified: November 2, 2016
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.